Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (4): 35-41.doi: 10.6040/j.issn.1671-7554.0.2020.1753

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Effects of curcumin on progesterone resistance in endometrial carcinoma

DING Fei, JIANG Jie   

  1. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2021-04-30

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Abstract: Objective To explore the effects of different concentrations of curcumin on progesterone resistant cells in endometrial carcinoma and to investigate the role of related signaling pathways influencing progesterone resistance. Methods The target genes of curcumin were analyzed in the ETCM and GEPIA databases and the interaction genes related to tumor-drug resistance were screened out. The resistance of an established progesterone-resistance model of Ishikawa cells in endometrial carcinoma(IshikawaPR cell line)was detected. Resistant cells were divided into curcumin(0, 5, 10, 20, 40, 60, 80 μmoL)groups, progesterone(0, 5, 10, 20, 40, 60, 90 μmoL)groups and curcumin+progesterone(the same concentration)groups, according to different treatments. The proliferation of cells in each group was determined with MTT. The effects of curcumin on the resistant cells were assessed with EDU, Transwell and mitochondrial membrane potential. The changes of intracellular protein levels after curcumin stimulation at different concentrations were determined with Western blotting. The potential signaling pathways were analyzed in the databases. Results Analyses of the databases indicated that curcumin target genes were closely related to tumor-drug resistance genes such as PGR and ESR. MTT results showed that curcumin inhibited the growth of drug-resistant cells, leading to a decrease in cell survival rate(F=907.8, P<0.001), and there was an interactive relationship between curcumin and progesterone(F=51.34, P=0.004). When the concentrations of progesterone were 10 μmoL(t=16.13, P=0.026), 20 μmoL(t=33.23, P=0.006), and 40μmoL(t=35.94, P=0.005), combination with curcumin inhibited drug-resistant cells more effectively than the use of progesterone alone. Curcumin treatment(0, 10, 20 μmoL)inhibited the proliferation(F=205.8, P<0.001), invasion(F=13.9, P=0.006)and promoted apoptosis(F=23.3, P=0.002)of drug-resistant cells. Western blotting showed that after curcumin treatment, the levels of CyclinD1, CASP3, MMP2 and p-NFκB decreased while the level of PR increased in resistant cells. Conclusion Curcumin inhibits progesterone resistance in endometrial carcinoma, possibly by mediating inactivation of transcription factor NFκB.

Key words: Curcumin, Endometrial carcinoma, Progesterone, Resistance, Nuclear transcription factor-κB

CLC Number: 

  • R711
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