Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (4): 84-89.doi: 10.6040/j.issn.1671-7554.0.2019.1388

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Ribosomal protein S6 kinase 2 silencing in ethanol-induced myocardial insulin resistance

XU Hongzhao1, HAO Zongshan2, SHEN Deqiang3, LI Zhaoping4, CHEN Liyong4   

  1. 1. Department of Toxicology and Nutrition, School of Public Health, Shandong University, Jinan 250012, Shandong, China;
    2. Shandong Provincial ENT Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    3. Department of Clinical Nutrition, Lianyungang Hospital Affiliated to Xuzhou Medical University, Lianyungang 222002, Jiangsu, China;
    4. Department of Nutrition, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the role of ribosomal protein S6 kinase 2(S6K2)in ethanol-induced myocardial insulin resistance. Methods The glucose uptake capacity of H9c2 cells after 100 mmol ethanol stimulation and lentivirus transfection was detected with fluorescence labeling 2-deoxy-2-［(7-nitro-2,1,3-benzoxadiazol-4-yl)amino］-D-glucose(2-NDBG)intake test and glucose oxidase. The expression of S6K2 and phosphorylation of insulin receptor substrate 1(p-IRS-1)were determined with Western blotting. The silencing of S6K2 after lentivirus transfection was detected with real time PCR and Western blotting. Results Ethanol exposure impaired glucose uptake in H9c2 cells. The mean fluorescence intensity of 2-NDBG decreased by 46.5%(P=0.016), the protein expression of p-IRS-1(Ser)increased by 14.4%(P=0.024), and the mRNA and protein expressions of S6K2 increased by 16.8% and 23.5% respectively(P=0.009, P=0.029). After S6K2 silencing, the difference of glucose uptake and p-IRS-1(Ser)expression in ethanol-stimulated H9c2 cells had no statistics significance(P=0.665, P=0.740). Conclusion Ethanol exposure triggers glucose intolerance and insulin resistance in H9c2 cells, and S6K2 silencing can alleviate impaired glucose uptake and insulin sensitivity induced by ethanol.

Key words: Ethanol, Myocardium, Insulin resistance, H9c2 cardiomyocyte, Ribosomal protein S6 kinase 2

CLC Number: 

  • R542.2
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