Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (11): 41-47.doi: 10.6040/j.issn.1671-7554.0.2021.0733

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Correlation between insulin resistance and expressions of local inflammatory factors and glucose transporter protein type-4 in the endometrium of patients with polycystic ovary syndrome

HA Lingxia1,2, YIN Ting1,2, WU Yangyang1,2, LI Weixia1,2, DU Yudong1,2   

  1. 1. Center for Reproductive Medicine, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan 750004, Ningxia, China
  • Published:2021-11-11

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Abstract: Objective To explore the expressions of local inflammatory factors and glucose transporter protein type-4(GLUT-4)in the endometrium of patients with polycystic ovary syndrome(PCOS), and to analyze the correlation between insulin resistance(IR)and the expressions of inflammatory factors and GLUT-4. Methods A total of 60 PCOS patients treated during June 2018 and July 2020 were enrolled. Based on the homeostasis model assessment for insulin resistance index(HOMA-IR), the patients were divided into IR PCOS group(n=30)and non-IR PCOS group(n=30). Meanwhile, 60 non-PCOS patients were collected and divided into IR group(n=30)and normal control group(n=30). Follicle stimulating hormone(FSH), luteining hormone(LH), estradiol(E2), testosterone(T), prolactin(PRL)and anti-Mullerian hormone(AMH)were detected. The expressions of tumor necrosis factor alpha(TNF-α), nuclear factor-kappa B(NF-κB)and GLUT-4 in the endometrium were detected with immunohistochemistry. Results (1) There were significant differences in body mass index(BMI), AMH, LH and T among the 4 groups(F=57.35, 29.54, 16.77, 21.20, P<0.001). Group A had higher BMI than group B; group C had higher BMI than group D. Groups A and B had higher levels of AMH and LH than groups C and D. Group A had higher T than group B. (2) There were significant differences in expressions of TNF-α, NF-κBp65 and GLUT-4 among the 4 groups(F=76.90, 45.13, 47.75, P<0.001). Group A had higher expressions of TNF-α and NF-κBp65 but lower GLUT-4 than group B. Group C had higher expressions of TNF-α and NF-κBp65 but lower expression of GLUT-4 than group D. (3)The results of multivariate Logistic regression analysis showed that HOMA-IR was positively correlated with the expression of NF-κBp65 but negatively correlated with the expression of GLUT-4 in PCOS and non-PCOS patients(P<0.05); HOMA-IR was positively correlated with BMI in non-PCOS patients(P<0.05). Conclusion Insulin resistance is associated with NF-κBp65 and GLUT-4 in PCOS patients. It may increase the expressions of local inflammatory factors and decrease the expression of GLUT-4 in the endometrium.

Key words: Polycystic ovary syndrome, Insulin resistance, Nuclear factor-κB, Tumor necrosis factor-alpha, Glucose transporter protein type-4

CLC Number: 

  • R711.71
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