Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (5): 116-120.doi: 10.6040/j.issn.1671-7554.0.2019.0148

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Expression and significance of microRNA-141 in granulosa cells of patients with polycystic ovary syndrome

HE Tingting1, LIU Hong1, SHU Kaiyun1, SONG Xiaocui2, SHI Yuhua1   

  1. 1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory for Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine;
    Shandong Provincial Clinical Medicine Research Center for Reproductive Health, Jinan 250001, Shandong, China;
    2. Department of Reproductive Medicine, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the expression of microRNA-141(miR-141)in ovarian granulosa cells of patients with polycystic ovary syndrome(PCOS)and its clinical significance. Methods A total of 70 PCOS patients were divided into normal-BMI group(BMI<25, n=42) and obesity group(BMI≥25, n=28)according to their(BMI), and 71 controls were divided into normal-BMI group(BMI<25, n=60)and obesity group(BMI≥25, n=11). The expression of miR-141 in ovarian granulosa cells of each group was detected with real-time quantitative reverse transcription PCR(qRT-PCR), and its clinical significance was analyzed. Results The expression of miR-141 was higher in the normal-BMI PCOS group than in the normal-BMI control group (P<0.001); and compared with obesity control group, higher expression of miR-141 was observed in obesity PCOS group (P=0.009). In addition, the incidence of 山 东 大 学 学 报 (医 学 版)57卷5期 -贺婷婷,等.多囊卵巢综合征患者颗粒细胞中microRNA-141的表达与意义 \=-pregnancy complications in PCOS patients tended to be higher, but there was no statistical significance(P>0.0125). Conclusion The expression of miR-141 is higher in the PCOS groups than in the control groups. and the risk of pregnancy complications tends to increase in PCOS patients, suggesting that miR-141 may play an important role in the pathogenesis of PCOS.

Key words: Polycystic ovary syndrome, Granulosa cell, MicroRNA-141, Pregnancy complications

CLC Number: 

  • R711.75
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