Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (4): 42-47.doi: 10.6040/j.issn.1671-7554.0.2021.0056

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Effect of testosterone on the expression of polycystic ovary syndrome susceptibility gene Tox3 in zebrafish

HAN Xiao, LI Lei, LIU Congcong   

  1. Center for Reproductive Medicine, Shandong University;
    Key Laboratory for Reproductive Endocrinology of Education Ministry;
    Shandong Provincial Key Laboratory of Reproductive Medicine;
    National Research Center for Assisted Reproduction and Reproductive Genetics, Jinan 250001, Shandong, China
  • Published:2021-04-30

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Abstract: Objective To determine the expression of polycystic ovary syndrome(PCOS)susceptibility gene Tox3 in wild-type zebrafish, and explore the effect of testosterone on Tox3 gene expression. Methods About 200 zebrafish embryos and 100 male and female adult zebrafish for control treatment and testosterone(10 ng/mL)treatment were required. Reverse transcription-polymerase chain reaction(RT-PCR)and real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)were used to test the expression of Tox3 gene in zebrafish. The distributions of Tox3 mRNA in zebrafish embryos were observed by whole mount in situ hybridization(WISH). Results In wild-type zebrafish, the level of Tox3 gene expression was high in the nervous system and reproductive system. After treated with 10 ng/mL testosterone, compared with the control group, the Tox3 gene expression of male zebrafish treated with 10 ng/mL testosterone was higher in brain ［(1.01±0.19) vs(1.43±0.35),P=0.043］ and in nest ［(1.05±0.32) vs(1.50±0.30),P=0.015］. Conclusion Tox3 in zebrafish may regulates the synthesis and release of androgen by acting on neuroendocrine systems.

Key words: Testosterone, Polycystic ovary syndrome, Tox3, Gene expression, Zebrafish

CLC Number: 

  • R711
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