Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (4): 48-55.doi: 10.6040/j.issn.1671-7554.0.2020.1752

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Value of MnFe2O4@CNS in the theragnostic of pancreatic cancer

ZHANG Gaorui1, ZHANG Yuting2, ZHAO Yuxuan3, WANG Fangqing3, YU Dexin3   

  1. 1. School of Medical Imaging, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Cardiology, Weifang Peoples Hospital, Weifang 261041, Shandong, China;
    3. Department of Radiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2021-04-30

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Abstract: Objective To investigate the value of MnFe2O4-loaded caramelized carbonaceous nanospheres(MnFe2O4@CNS)with inducing ferroptosis and synchronous MR monitoring in the theragnostic of pancreatic cancer. Methods After MnFe2O4@CNS was prepared, the characterization and relaxivity were detected. The cytotoxicity was determined with cell counting kit-8(CCK-8)assay. The Fenton reaction catalytic activity of different concentrations of MnFe2O4@CNS were evaluated. The uptake of MnFe2O4@CNS by murine pancreatic adenocarcinoma(Panc02)cells was detected. The imaging ability of MnFe2O4@CNS in Panc02 cells was evaluated. After incubation with MnFe2O4@CNS, the levels of reactive oxygen species(ROS)and cell death of Panc02 cells were evaluated and the morphological changes were observed with transmission electron microscopy(TEM). Results The average particle size of MnFe2O4@CNS was 183nm, and the relaxivity was 512.58 mmol/L·s-1. After incubation with 50, 100 and 200 μg/mL MnFe2O4@CNS, the cell viability of the human pancreatic ductal epithelial cells(hTERT-HPNE)and Panc02 were significantly different(P<0.05). The Fenton reaction of MnFe2O4@CNS was high and MnFe2O4@CNS could be ingested by cancer cells. With higher concentration of MnFe2O4@CNS, the levels of ROS and apoptosis of Panc02 cells increased gradually, and the mitochondrial morphology of Panc02 cells showed classical characteristics of ferroptosis. The MRI of Panc02 cells showed high contrast signal effect of T2 with as incubation time increased(P<0.05). Conclusion Highly biocompatible, MnFe2O4@CNS can achieve targeted MRI and induce ferroptosis of pancreatic neoplasms, which is of great value in the theragnostic of pancreatic cancer.

Key words: Pancreatic cancer, Fenton reaction, Ferroptosis, Magnetic resonance imaging, Theragnostic

CLC Number: 

  • R445.2
[1] Xu M, Jung X, Hines O J, et al. Obesity and pancreatic cancer: overview of epidemiology and potential prevention by weight loss [J]. Pancreas, 2018, 47(2): 158-162.
[2] Gajiwala S, Torgeson A, Garrido-Laguna I, et al. Combination immunotherapy and radiation therapy strategies for pancreatic cancer-targeting multiple steps in the cancer immunity cycle [J]. J Gastrointest Oncol, 2018, 9(6): 1014-1026.
[3] Saravanakumar K, Hu X, Ali D M, et al. Emerging strategies in stimuli-responsive nanocarriers as the drug delivery system for enhanced cancer therapy [J]. Curr Pharm Des, 2019, 25(24): 2609-2625.
[4] Xu T, Ding W, Ji X, et al. Molecular mechanisms of ferroptosis and its role in cancer therapy [J]. J Cell Mol Med, 2019, 23(8): 4900-4912.
[5] Dixon SJ, Lemberg KM, Lamprecht MR, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death [J]. Cell, 2012, 149(5): 1060-1072.
[6] Shen Z, Song J, Yung BC, et al. Emerging strategies of cancer therapy based on ferroptosis [J]. Adv Mater, 2018, 30(12): e1704007.doi:10.1002/adma.201704007
[7] Morris JT, Wang SC, Hebrok M. KRAS, Hedgehog, Wnt and the twisted developmental biology of pancreatic ductal adenocarcinoma [J]. Nat Rev Cancer, 2010, 10(10): 683-695.
[8] Ansari D, Carvajo M, Bauden M, et al. Pancreatic cancer stroma: controversies and current insights [J]. Scand J Gastroenterol, 2017, 52(6-7): 641-646.
[9] Stathis A, Moore MJ. Advanced pancreatic carcinoma: current treatment and future challenges [J]. Nat Rev Clin Oncol, 2010, 7(3): 163-172.
[10] Arlt A, Muerkoster SS, Schafer H. Targeting apoptosis pathways in pancreatic cancer [J]. Cancer Lett, 2013, 332(2): 346-358.
[11] Wolske KM, Ponnatapura J, Kolokythas O, et al. Chronic pancreatitis or pancreatic tumor? A problem-solving approach [J]. Radiographics, 2019, 39(7): 1965-1982.
[12] Feng W, Han X, Wang R, et al. Nanocatalysts-augmented and photothermal-enhanced tumor-specific sequential nanocatalytic therapy in both NIR-I and NIR-II biowindows [J]. Adv Mater, 2019, 31(5): e1805919. doi:10.1002/adma.201805919
[13] Gao M, Yi J, Zhu J, et al. Role of Mitochondria in Ferroptosis [J]. Mol Cell, 2019, 73(2): 354-363.
[14] Zheng DW, Lei Q, Zhu JY, et al. Switching apoptosis to ferroptosis: metal-organic network for high-efficiency anticancer therapy [J]. Nano Lett, 2017, 17(1): 284-291.
[15] Sabharwal SS, Schumacker PT. Mitochondrial ROS in cancer: initiators, amplifiers or an Achilles heel? [J]. Nat Rev Cancer, 2014, 14(11): 709-721.
[16] Baulies A, Montero J, Matias N, et al. The 2-oxoglutarate carrier promotes liver cancer by sustaining mitochondrial GSH despite cholesterol loading [J]. Redox Biol, 2018, 14(1): 164-177.
[17] Gong N, Ma X, Ye X, et al. Carbon-dot-supported atomically dispersed gold as a mitochondrial oxidative stress amplifier for cancer treatment [J]. Nat Nanotechnol, 2019, 14(4): 379-387.
[18] Yang B, Liu Q, Yao X, et al. FePt@MnO-Based Nanotheranostic platform with acidity-triggered dual-Ions release for enhanced MR imaging-guided ferroptosis chemodynamic terapy [J]. ACS Appl Mater Interfaces, 2019, 11(42): 38395-38404.
[19] Shen Z, Liu T, Li Y, et al. Fenton-reaction-acceleratable magnetic nanoparticles for ferroptosis therapy of prthotopic brain tumors [J]. ACS Nano, 2018, 12(11): 11355-11365.
[20] Bebber CM, Muller F, Prieto CL, et al. Ferroptosis in cancer cell biology [J]. Cancers(Basel), 2020, 12(1): 164.
[21] Xiang Y, Li N, Guo L, et al. Biocompatible and pH-sensitive MnO-loaded carbonaceous nanospheres(MnO@CNSs): a theranostic agent for magnetic resonance imaging-guided photothermal therapy [J]. Carbon, 2018, 136(1): 113-124.
[22] Qi Y, Li W, Fang J, et al. Application and mechanism of manganese-coated caramelization nanospheres for active targeting in hepatobiliary tumors [J]. Nanomedicine(Lond), 2019, 14(22): 2973-2985.
[23] Silambarasu A, Manikandan A, Balakrishnan K, et al. Comparative Study of Structural, Morphological, Magneto-Optical and Photo-Catalytic Properties of Magnetically Reusable Spinel MnFe2O4 Nano-Catalysts[J]. J Nanosci Nanotechnol, 2018, 18(5): 3523-3531.
[24] Huang G, Li H, Chen J, et al. Tunable T1 and T2 contrast abilities of manganese-engineered iron oxide nanoparticles through size control [J]. Nanoscale, 2014, 6(17): 10404-10412.
[25] 陶旭锋,Vay Liang W. Bill Go,肖桂山. 活性氧介导的肿瘤微环境在胰腺癌发生与发展中作用的研究进展[J]. 生理学报, 2021(11): 1-22. TAO Xufeng, Vay Liang W. Bill Go, XIAO Guishan. Rogress on the role of reactive oxygen species-mediated tumor microenvironment in pancreatic cancer [J]. Acta Physiologica Sinica, 2021(11): 1-22.
[26] Bao W, Liu X, Lv Y, et al. Nanolongan with multiple on-demand conversions for ferroptosis-apoptosis combined anticancer therapy [J]. ACS Nano, 2019, 13(1): 260-273.
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