Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (4): 48-55.doi: 10.6040/j.issn.1671-7554.0.2020.1752

Previous Articles     Next Articles

Value of MnFe2O4@CNS in the theragnostic of pancreatic cancer

ZHANG Gaorui1, ZHANG Yuting2, ZHAO Yuxuan3, WANG Fangqing3, YU Dexin3   

  1. 1. School of Medical Imaging, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Cardiology, Weifang Peoples Hospital, Weifang 261041, Shandong, China;
    3. Department of Radiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2021-04-30

PDF (PC)

123

Abstract: Objective To investigate the value of MnFe2O4-loaded caramelized carbonaceous nanospheres(MnFe2O4@CNS)with inducing ferroptosis and synchronous MR monitoring in the theragnostic of pancreatic cancer. Methods After MnFe2O4@CNS was prepared, the characterization and relaxivity were detected. The cytotoxicity was determined with cell counting kit-8(CCK-8)assay. The Fenton reaction catalytic activity of different concentrations of MnFe2O4@CNS were evaluated. The uptake of MnFe2O4@CNS by murine pancreatic adenocarcinoma(Panc02)cells was detected. The imaging ability of MnFe2O4@CNS in Panc02 cells was evaluated. After incubation with MnFe2O4@CNS, the levels of reactive oxygen species(ROS)and cell death of Panc02 cells were evaluated and the morphological changes were observed with transmission electron microscopy(TEM). Results The average particle size of MnFe2O4@CNS was 183nm, and the relaxivity was 512.58 mmol/L·s-1. After incubation with 50, 100 and 200 μg/mL MnFe2O4@CNS, the cell viability of the human pancreatic ductal epithelial cells(hTERT-HPNE)and Panc02 were significantly different(P<0.05). The Fenton reaction of MnFe2O4@CNS was high and MnFe2O4@CNS could be ingested by cancer cells. With higher concentration of MnFe2O4@CNS, the levels of ROS and apoptosis of Panc02 cells increased gradually, and the mitochondrial morphology of Panc02 cells showed classical characteristics of ferroptosis. The MRI of Panc02 cells showed high contrast signal effect of T2 with as incubation time increased(P<0.05). Conclusion Highly biocompatible, MnFe2O4@CNS can achieve targeted MRI and induce ferroptosis of pancreatic neoplasms, which is of great value in the theragnostic of pancreatic cancer.

Key words: Pancreatic cancer, Fenton reaction, Ferroptosis, Magnetic resonance imaging, Theragnostic

CLC Number: 

  • R445.2
[1] Xu M, Jung X, Hines O J, et al. Obesity and pancreatic cancer: overview of epidemiology and potential prevention by weight loss [J]. Pancreas, 2018, 47(2): 158-162.
[2] Gajiwala S, Torgeson A, Garrido-Laguna I, et al. Combination immunotherapy and radiation therapy strategies for pancreatic cancer-targeting multiple steps in the cancer immunity cycle [J]. J Gastrointest Oncol, 2018, 9(6): 1014-1026.
[3] Saravanakumar K, Hu X, Ali D M, et al. Emerging strategies in stimuli-responsive nanocarriers as the drug delivery system for enhanced cancer therapy [J]. Curr Pharm Des, 2019, 25(24): 2609-2625.
[4] Xu T, Ding W, Ji X, et al. Molecular mechanisms of ferroptosis and its role in cancer therapy [J]. J Cell Mol Med, 2019, 23(8): 4900-4912.
[5] Dixon SJ, Lemberg KM, Lamprecht MR, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death [J]. Cell, 2012, 149(5): 1060-1072.
[6] Shen Z, Song J, Yung BC, et al. Emerging strategies of cancer therapy based on ferroptosis [J]. Adv Mater, 2018, 30(12): e1704007.doi:10.1002/adma.201704007
[7] Morris JT, Wang SC, Hebrok M. KRAS, Hedgehog, Wnt and the twisted developmental biology of pancreatic ductal adenocarcinoma [J]. Nat Rev Cancer, 2010, 10(10): 683-695.
[8] Ansari D, Carvajo M, Bauden M, et al. Pancreatic cancer stroma: controversies and current insights [J]. Scand J Gastroenterol, 2017, 52(6-7): 641-646.
[9] Stathis A, Moore MJ. Advanced pancreatic carcinoma: current treatment and future challenges [J]. Nat Rev Clin Oncol, 2010, 7(3): 163-172.
[10] Arlt A, Muerkoster SS, Schafer H. Targeting apoptosis pathways in pancreatic cancer [J]. Cancer Lett, 2013, 332(2): 346-358.
[11] Wolske KM, Ponnatapura J, Kolokythas O, et al. Chronic pancreatitis or pancreatic tumor? A problem-solving approach [J]. Radiographics, 2019, 39(7): 1965-1982.
[12] Feng W, Han X, Wang R, et al. Nanocatalysts-augmented and photothermal-enhanced tumor-specific sequential nanocatalytic therapy in both NIR-I and NIR-II biowindows [J]. Adv Mater, 2019, 31(5): e1805919. doi:10.1002/adma.201805919
[13] Gao M, Yi J, Zhu J, et al. Role of Mitochondria in Ferroptosis [J]. Mol Cell, 2019, 73(2): 354-363.
[14] Zheng DW, Lei Q, Zhu JY, et al. Switching apoptosis to ferroptosis: metal-organic network for high-efficiency anticancer therapy [J]. Nano Lett, 2017, 17(1): 284-291.
[15] Sabharwal SS, Schumacker PT. Mitochondrial ROS in cancer: initiators, amplifiers or an Achilles heel? [J]. Nat Rev Cancer, 2014, 14(11): 709-721.
[16] Baulies A, Montero J, Matias N, et al. The 2-oxoglutarate carrier promotes liver cancer by sustaining mitochondrial GSH despite cholesterol loading [J]. Redox Biol, 2018, 14(1): 164-177.
[17] Gong N, Ma X, Ye X, et al. Carbon-dot-supported atomically dispersed gold as a mitochondrial oxidative stress amplifier for cancer treatment [J]. Nat Nanotechnol, 2019, 14(4): 379-387.
[18] Yang B, Liu Q, Yao X, et al. FePt@MnO-Based Nanotheranostic platform with acidity-triggered dual-Ions release for enhanced MR imaging-guided ferroptosis chemodynamic terapy [J]. ACS Appl Mater Interfaces, 2019, 11(42): 38395-38404.
[19] Shen Z, Liu T, Li Y, et al. Fenton-reaction-acceleratable magnetic nanoparticles for ferroptosis therapy of prthotopic brain tumors [J]. ACS Nano, 2018, 12(11): 11355-11365.
[20] Bebber CM, Muller F, Prieto CL, et al. Ferroptosis in cancer cell biology [J]. Cancers(Basel), 2020, 12(1): 164.
[21] Xiang Y, Li N, Guo L, et al. Biocompatible and pH-sensitive MnO-loaded carbonaceous nanospheres(MnO@CNSs): a theranostic agent for magnetic resonance imaging-guided photothermal therapy [J]. Carbon, 2018, 136(1): 113-124.
[22] Qi Y, Li W, Fang J, et al. Application and mechanism of manganese-coated caramelization nanospheres for active targeting in hepatobiliary tumors [J]. Nanomedicine(Lond), 2019, 14(22): 2973-2985.
[23] Silambarasu A, Manikandan A, Balakrishnan K, et al. Comparative Study of Structural, Morphological, Magneto-Optical and Photo-Catalytic Properties of Magnetically Reusable Spinel MnFe2O4 Nano-Catalysts[J]. J Nanosci Nanotechnol, 2018, 18(5): 3523-3531.
[24] Huang G, Li H, Chen J, et al. Tunable T1 and T2 contrast abilities of manganese-engineered iron oxide nanoparticles through size control [J]. Nanoscale, 2014, 6(17): 10404-10412.
[25] 陶旭锋,Vay Liang W. Bill Go,肖桂山. 活性氧介导的肿瘤微环境在胰腺癌发生与发展中作用的研究进展[J]. 生理学报, 2021(11): 1-22. TAO Xufeng, Vay Liang W. Bill Go, XIAO Guishan. Rogress on the role of reactive oxygen species-mediated tumor microenvironment in pancreatic cancer [J]. Acta Physiologica Sinica, 2021(11): 1-22.
[26] Bao W, Liu X, Lv Y, et al. Nanolongan with multiple on-demand conversions for ferroptosis-apoptosis combined anticancer therapy [J]. ACS Nano, 2019, 13(1): 260-273.
[1] JIANG Hui, WEI Tian, LI Jianping, WANG Cong. Protective effects and molecular mechanism of puerarin on sorafenib-induced cardiotoxicity [J]. Journal of Shandong University (Health Sciences), 2022, 60(8): 14-22.
[2] Guowei TAO,Fang WANG,Xiangyi DONG,Yaxuan XU,Linli ZHAO,Beibei HU. Diagnosis of adenomyosis using ultrasound and magnetic resonance imaging [J]. Journal of Shandong University (Health Sciences), 2022, 60(7): 56-65.
[3] SUN Haoyu, JIANG Xin, CHEN Shouzhen, QU Sifeng, SHI Benkang. Multiparametric magnetic resonance imaging combined with prostate health index in the diagnosis of prostate cancer in 142 patients within PSA grey zone [J]. Journal of Shandong University (Health Sciences), 2022, 60(6): 46-50.
[4] ZUO Liping, JIANG Fengyang, ZHOU Binbin, FAN Jinlei, LIANG Yongfeng, DENG Zhanhao, YU Dexin. Value of preoperative multiphase MRI for predicting microvascular invasion and early recurrence of 169 hepatocellular carcinoma [J]. Journal of Shandong University (Health Sciences), 2022, 60(3): 89-95.
[5] FENG Baomin, WANG Zhou, XU Han, LI Jiacun, YU Qiaowen, XIU Jianjun. Clinical and imaging features of 19 cases of anti-myelin oligodendrocyte glycoprotein-IgG antibody associated disorders [J]. Journal of Shandong University (Health Sciences), 2022, 60(3): 45-50.
[6] YUAN Baowen, WANG Pei, HUANG Wei. Regulatory role of histone deacetylase SIRT1 in pancreatic cancer metabolism [J]. Journal of Shandong University (Health Sciences), 2022, 60(3): 1-12.
[7] LI Yanru, LI Juan, LI Peilong, DU Lutao, WANG Chuanxin. Proteomic analysis of serum exosomes in pancreatic cancer with different stages of progress [J]. Journal of Shandong University (Health Sciences), 2022, 60(10): 33-41.
[8] LIU Xueye, LI Qiming, TANG Hongyi, XU Qiuping, CHEN Wenqian, GUO Jing. Relationship among the volume and surface area of the temporomandibular joint condyle and the sagittal position of disc in young adults [J]. Journal of Shandong University (Health Sciences), 2021, 59(6): 117-121.
[9] SUN Dingqi, FU Qiang, ZHANG Hui, LIU Shuai, DIAO Tongxiang, CAO Qingwei, ZHANG Keqin. Clinical value of targeted multiparameter MRI and transrectal ultrasonography fusion prostate biopsy for the detection of PI-RADS≥3 prostate cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(4): 108-113.
[10] SONG Zhenzhen, SUN Xiaoling, LI Haiou, WANG Fang, ZHANG Ran, YU Dexin. Value of MRI radiomics of glioma and peritumoral edema in evaluating tumor recurrence [J]. Journal of Shandong University (Health Sciences), 2021, 59(11): 53-60.
[11] CHEN Xiaoli, GUI Zhenchao, GAO Yang, XING Mengyao, XIU Jianjun. Imaging findings of 13 cases of salivary duct carcinoma [J]. Journal of Shandong University (Health Sciences), 2021, 59(1): 78-82.
[12] LU Jingjing, XIA Yu. Imaging diagnosis of endometriosis [J]. Journal of Shandong University (Health Sciences), 2019, 57(6): 40-45.
[13] ZHANG Xiaoqian, MENG Xiangshui, REN Qingguo, NAN Xiaomin, AN Panpan, SHUAI Xinyan, XIA Xiaona, WANG Xuan. Magnetic resonance spectroscopy in patients with vascular cognitive impairment no dementia [J]. Journal of Shandong University (Health Sciences), 2019, 57(4): 42-46.
[14] ZHANG Lihong, WANG Linsheng, CHEN Dongfeng, CHEN Yueqin, LI Xian, LIU Yanjie, LI Lei. CT and MRI appearances of mixed epithelial and stromal tumor of kidney [J]. Journal of Shandong University (Health Sciences), 2018, 56(7): 70-75.
[15] ZHANG Lihong, LI Xian, WANG Linsheng, LI Honglei, LI Lei. Fat-containing ganglioneuroma: imaging features and clinicopathological correlation [J]. Journal of Shandong University (Health Sciences), 2018, 56(12): 73-78.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] ZHENG Min,HAO Yue-wei,LIU Xue-ping,ZHAO Ting-ting. Relationship between the HPA-2 and Kozak sequence polymorphism of platelet membrane glycoprotein and cerebral infarction[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(3): 292 -295 .
[2] ZHANG Jie,LI Zhen-hua,SUN Jin-hao,BAO Li-hua,LIU Yue-peng. Protective effects of the invariable magnetic field on oxidative injured Schwann cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(3): 229 -232 .
[3] . [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(10): 995 .
[4] JIANG Hong-ju,LI Run-zhi, WANG Ying,XU Dong-mei,ZHANG Mei,ZHANG Yun,LI Ji-fu. Relationship between the plasma level of matrix metalloproteinase-9 and structural characteristics of plaques and percutaneous coronary intervention [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(10): 966 -970 .
[5] WANG Shu-qin,QI Feng,WU Jian-bo,SUN Bao-zhu. Effect of calcium in ropivacaine-induced smooth muscle contraction of the aorta in rats in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(8): 773 -776 .
[6] TENG Xue-ren,ZHAO Yong-sheng,HU Guang-liang,ZHOU Lun,LI Jian-min. Characteristics of microcosmic changes following tendon allograft transplantation preserved by two methods under light microscopy and transmission electron microscopy[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(10): 945 -950 .
[7] WU Min,ZHAO Zhi-lun,WANG Jie-zhen. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(11): 1146 -1149 .
[8] . [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(8): 828 -830 .
[9] . [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(9): 5 -6 .
[10] NIU Rui,LIU Bo,SHAO Ming-ju,WANG Wei. mRNA expression of lunx in regional lymph nodes of non-small cell lung cancer and its correlation with prognosis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(9): 884 -885 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd