JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (12): 8-13.doi: 10.6040/j.issn.1671-7554.0.2015.1231

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Effects of EGCG on the expressions of hippocampal amyloid beta protein and neprilysin enzyme in C57BL/6 young mice exposed to lead

YIN Yanyan1,2, ZHAO Linyuan1,3, ZHANG Xiaohuan1, HUANG Hui1, SUN Beibei1, LI Wenjie1   

  1. 1. College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China;
    2. College of Basic Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China;
    3.Department of Clinical Nutrition, The first Afficiated Hospital, Luohe Medical College, Luohe 462000 Henan, China
  • Received:2015-12-02 Online:2016-12-10 Published:2016-12-10

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Abstract: Objective To explore the effects of different doses of epigallocatechin 3 gallate, table gallic catechin gallic acid ester(EGCG)on the learning and memory ability, amyloid beta and neprilysin enzymes in lactation mice which were exposed to lead. Methods SPF pregnant C57BL/6 mice were divided into control group(drank distilled water, n=4)and lead exposure group(drank 0.5% Pb-contaminated acetic acid solution, n=14). Another 40 male lead-exposed mice were divided into 4 groups, low EGCG group(1.5 mg/kg), which are moderate EGCG group(3.0 mg/kg), 山 东 大 学 学 报 (医 学 版)54卷12期 -尹延彦,等.EGCG对铅暴露C57BL/6仔鼠海马Aβ蛋白及NEP酶表达的影响 \=-high EGCG group(4.5 mg/kg)and lead exposure group, with 10 mice in each group. Another 10 male mice served as the control group(normal saline). After 21 days of treatment, all rats’ learning and memory ability was tested with Morris water maze experiment; the lead content in blood and hippocampus was determined with graphite furnace atomic absorption spectrometer; the protein contents of Aβ1-40, Aβ1-42, AβPP mRNA, AβPP and the neprilysin enzyme were also detected. Results The lead exposure group, and low, moderate and high EGCG groups had significantly higher content of blood lead than the control group(P<0.05). There was no difference among EGCG groups and lead exposure group(P=0.174, 0.086, 0.071). Lead exposure group had longer escaping latency than the control group(P<0.001), while the EGCG group had shorter escaping latency than the control group(P<0.001). Compared with the control group, the lead exposure group had increased content of Aβ1-40 and Aβ1-42(P<0.001), and decreased Nep content(P<0.001); the moderate and high EGCG groups had decreased Aβ1-40 and Aβ1-42 content and increased Nep content(P<0.001), and the high EGCG group had better interventional effect. Conclusion EGCG intervention can significantly improve the learning and memory ability of mice. EGCG can reduce Aβ1-40 and Aβ1-42 contents in the hippocampus, inhibit the expression of related gene AβPP, and upregulate the Nep protein expression.

Key words: Lead exposure in early life, Beta-amyloid precursor protein, Aβ1-40, Neprilysin, Epigallocatechin-3-gallate, Aβ1-42

CLC Number: 

  • R135.1+1
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