枯草芽孢杆菌灌胃对小鼠肠道菌群的影响

doi:10.6040/j.issn.1671-7554.0.2017.076

Abstract

Abstract: Objective To investigate the effect of Bacillus subtilis B.subtilis gavage on the intestinal microbiota of mice. Methods Eighteen C57BL/6J female mice were randomly divided into three groups including control group(C group), two days intervention group(TW group)and ten days intervention group(TE group). C group was treated with normal saline each day from day 0 to day 9; TW group was treated with live B.subtilis each day from day 0 to day 1, then with normal saline at the same amount from day 2 to day 9; TE group was treated with live B.subtilis at the same amount each day from day 0 to day 9. All mice were given an unrestricted diet from day 0 to day 16.Feces were collected on day 0, day 8 and day 16 to extract DNA, then Illumina Miseq platform was used for high-throughput sequencing. Results After treated with live B.subtilis, the relative abundance of Bacillus genus microbes in TW group and TE group decreased, and in the TE group the difference was significantly different(P=0.039). The relative abundance of Firmicutes increased first then decreased in both TW group and TE group, while Bacteroidetes shows reverse trend. The relative abundance of Verrucomicrobia increased gradually, which in TW group showed significant difference on the sixteenth day and eighth day compared with that before gavage(P=0.001). The relative abundances of Verrucomicrobia in TW group, TE group and C group on the sixteenth day were significantly different(P=0.007). Meanwhile, it was found that the intestinal flora structure of mice on the eighth day was similar to that on the sixteenth day in TE group, while the intestinal flora structure of mice in TW group differed siginificantly. On the sixteenth day, the intestinal flora structure of TW group was similar to that of TE group. Conclusion B.subtilis could regulate intestinal flora structure possibly via interacting with intestinal microbia but not via colonizing. Long-term use of the probiotic 山东大学学报 (医学版)55卷10期 -赵力,等.枯草芽孢杆菌灌胃对小鼠肠道菌群的影响 \=-changes intestinal flora structure more quickly than short-term does.

Key words: High-throughput sequencing, Probiotics, Intestinal microbiota, Bacillus subtilis

CLC Number:

R574

ZHAO Li, LI Lixiang, CHEN Feixue, ZUO Xiuli, LI Yanqing. Effect of Bacillus subtilis gavage on the intestinal microbiota of mice[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 28-35.

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Effect of Bacillus subtilis gavage on the intestinal microbiota of mice

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