JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (12): 46-52.doi: 10.6040/j.issn.1671-7554.0.2016.1146

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Diagnostic value of pepsinogen in atrophic gastritis and gastric cancer evaluation

ZHANG Shoucai1, GAO Yu2, ZHENG Guixi1, YANG Yongmei1, LIU Yanhong1, GAO Yang1, WANG Chuanxin1   

  1. 1. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Maternal and Child Health Care of Family Planning Service Center of Shibei District, Qingdao 266032, Shandong, China
  • Received:2016-09-11 Online:2016-12-10 Published:2016-12-10

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Abstract: Objective To evaluate the significance of pepsinogen in atrophic gastritis and gastric cancer using chemiluminesent immunoassay assay(CLIA)and enzyme linked immunosorbent assay(ELISA), and to analyze the consistency of the two methods. Methods CLIA and ELISA were used to determine the expressions of serum PG Ⅰ, PGⅡ and PG Ⅰ/Ⅱ(PGR)in healthy controls(n=95), non-atrophic gastritis cases(n=173), atrophic gastritis cases(n=407)and gastric cancer cases(n=135). The most suitable cut-off values of PG of the two methods were determined. Their diagnostic efficiencies were evaluated, and the consistency of CLIA and ELISA in detecting PG was analyzed. Results The expression of PG Ⅱ in gastric cancer group was higher than in atrophic gastritis group, non-atrophic gastritis group, and healthy controls(CLIA: P=0.027, 0.002, <0.001; ELISA: P=0.008, <0.001, <0.001). PGR in gastric cancer group was lower than in atrophic gastritis group, non-atrophic gastritis group, and healthy controls(CLIA: P<0.001, <0.001, <0.001; ELISA: P<0.001, <0.001, 0.001). PG Ⅱ>13.20 and PGR≤6.67 were considered the most suitable cut-off value for diagnosing gastric cancer using CLIA, and the sensitivity 山 东 大 学 学 报 (医 学 版)54卷12期 -张守彩,等.两种方法评估胃蛋白酶原在萎缩性胃炎和胃癌中的诊断价值 \=-and specificity were 80.88% and 71.64%, respectively. PG Ⅱ>18.10 and PGR≤6.18 were considered the most suitable cut-off value for diagnosing gastric cancer using ELISA, and the sensitivity and specificity were 80.14% and 62.72%, respectively. Further analysis showed that Kappa index of CLIA and ELISA in diagnosing gastric cancer and atrophic gastritis were 0.706 and 0.569, respectively. Conclusion PG Ⅱ and PGR can be used as useful biomarkers for the diagnosis of gastric cancer. The combination of PG Ⅱ and PGR has considerable diagnostic value of atrophic gastritis and gastric cancer with higher sensitivity and specificity. CLIA and ELISA have a good consistency in detecting PG.

Key words: Gastric cancer, Atrophic gastritis, Pepsinogen Ⅱ, Chemiluminesent immunoassay assay, Pepsinogen Ⅰ, Enzyme linked immunosorbent assay

CLC Number: 

  • R735.2
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