JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Establishment of cisplatin-resistant gastric cancer cell line and the biological characteristics

YAN Bing1, LIU Guoqin2, JIANG Yongsheng2, SHEN Haiyu2, SUN Shaochuan2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong， China;
    2. Department of Gastrointestinal Surgery, Jinan Central Hospital Affiliated to Shandong University,
    Jinan 250013, Shandong， China
  • Received:2013-09-12 Online:2014-04-10 Published:2014-04-10

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Abstract: Objective  To explore the drug-resistant gastric cancer cell line induced by high-dose  cDDP and its biological characteristics.  Methods  A drug-resistant gastric cancer cell line BGC823/cDDP was established by high-dose cDDP. The changes of biological characteristics of the cell lines were observed using light microscopy. The resistance index was determined by CCK-8 assay. The cell growth curve was drawn by CCK-8 assay. The cell cycle of drug-resistant cells was tested by flow cytometry. The cell invasion ability was observed by Transwell assay. The densities of CEA and CA-199 in the cell culture medium were evaluated by immune luminescence. Results  Compared with BGC823 cells, the BGC823/cDDP cells turned to be slightly bigger and irregular in shape, and some giant cells could be seen. The proliferation rate of BGC823/cDDP cell prolonged, with the resistance index being 4.54. The population doubling-time prolonged. The cell numbers of G1 phase increased while decreased in S and G2 phase in BGC823/cDDP cell. The invasion ability was stronger in BGC823/cDDP cell (P<0.05). The densities of CEA and CA-199 had no significant differences between BGC823 and BGC823/cDDP. Conclusion  The cDDP-resistant gastric cancer cell line BGC823/cDDP was induced by high-dose cDDP. The BGC-823 and BGC-823/cDDP cells were different in biological characteristics.

Key words: Biological characteristics, Gastric cancer, Drug-resistant cells, cDDP

CLC Number: 

  • R735.2
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