JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (11): 24-26.doi: 10.6040/j.issn.1671-7554.0.2015.542

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Effects of curcumin on gene expressions of reverse cholesterol transport proteins ABCA1versus ABCG1 in the cell model of inflammation

WU Yu1*, XIA Yanqing2*, WU Yue2, SUN Lin1, WU Chengye2, LOU Guiyu2, LI Yaxin2, DING Songze2, SUN Kai2   

  1. 1. Department of Kangxin Cardiology;
    2.Center for Translational Medicine, Henan Provincial Peoples Hospital, Zhengzhou 450003, Henan, China
  • Received:2015-06-04 Online:2016-11-10 Published:2016-11-10

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Abstract: Objective To explore the effects of curcumin on reverse cholesterol transport protein gene expression in the cell model of inflammation. Methods RAW264.7 cells were divided into 6 groups: control group, 10 μmol/L curcumin group, 20 μmol/L curcumin group, lipopolysaccharide(LPS)group, 10 μmol/L curcumin+LPS group, and 20 μmol/L curcumin+LPS group. The levels of mRNA expressions of ATP-binding cassette transporter A1(ABCA1), ATP-binding cassette transporter G1(ABCG1)and tumor necrosis factor-α(TNF-α)in each group were determined by Real-Time PCR. Results LPS induction significantly increased ABCA1 and TNF-α mRNA expressions, and decreased ABCG1 mRNA expression(all P<0.05). Pre-treating the cell with curcumin and stimulating with LPS did not change the expressions of TNF-α and ABCG1(both P>0.05), but significantly increased the ABCA1 mRNA expression(P<0.05). Conclusion Curcumin significantly amplifies the expression of reverse cholesterol transport key genes ABCA1 under the condition of the inflammatory reaction, which may be one of the molecular mechanisms of anti-arteriosclerosis.

Key words: Curcumin, Lipopolysaccharide, ATP-binding cassette transporter A1, Arteriosclerosis

CLC Number: 

  • R34
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