JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (11): 16-20.doi: 10.6040/j.issn.1671-7554.0.2014.880

• Preclinical Medicine • Previous Articles     Next Articles

Effects of cholecystokinin-octopeptide on endoplasmic reticulum stress induced by LPS in mouse monocyte RAW264.7 cells

DUAN Lin1, ZHANG Xiaojing2, YU Panpan2, YU Feng2, LIU Wei3, SONG Ning1   

  1. 1. Department of Respiratory Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China;
    2. Department of Forensic Medicine, Basic Medical College, Hebei Medical University, Shijiazhuang 050017, Hebei, China;
    3. Department of Orthopaedics, Hebei Chest Hospital, Shijiazhuang 050041, Hebei, China
  • Received:2014-11-28 Online:2015-11-10 Published:2015-11-10

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Abstract: Objective To investigate the effects of cholecystokinin-octopeptide (CCK-8) on ER stress (ERS) induced by lipopolysaccharide (LPS) in mouse monocyte RAW264.7 cells. Methods RAW264.7 cells were divided into control group, LPS group, CCK-8 grpup, devazepide group(DEV group), CCK-8+LPS group, and DEV+CCK-8+LPS group. The active form of X-box binding protein 1(XBP1s) mRNA expression was assayed by RT-PCR, glucose regulating protein 78 (GRP78) and C/EBP homologous protein (CHOP) protein expressions were determined by Western blotting, and IL-1β expression levels were measured by ELISA. Results Compared with control group, the expressions of XBP1s mRNA and GRP78 and CHOP protein in LPS group increased (P<0.05); cmpared with LPS group, the expressions of XBP1s mRNA and GRP78 and CHOP protein in CCK-8+LPS group decreased (P<0.05); compared with CCK-8+LPS group, the expressions of XBP1s mRNA and GRP78 and CHOP protein in DEV+CCK-8+LPS group increased (P<0.05). The changes of IL-1β contents showed the same trend as that of CHOP expression (P<0.05). Conclusion CCK-8 exerts an anti-inflammatory effect by inhibiting LPS-induced ERS and decreasing the expression of IL-1β in RAW264.7 cells.

Key words: Cholecystokinin-octopeptide, Lipopolysaccharides, X-box binding protein 1, C/EBP homologous protein, Glucose regulating protein 78

CLC Number: 

  • R541.4
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