JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 36-40.doi: 10.6040/j.issn.1671-7554.0.2014.909

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Expression of Hsp27 in lipopolysaccharide-induced injury of human gingival fibroblasts

ZHU Zhenkun, ZHOU Qin, SUN Ruinan, XU Xin   

  1. School of Stomatology of Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, Shandong, China
  • Received:2014-12-08 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To observe the expression of heat shock protein 27 (Hsp27) in injury of human gingival fibroblasts (HGFs) induced by lipopolysaccharide (LPS) with different concentrations. Methods The 3rd-8th generations of cultured HGFs were treated with serially diluted LPS. Would healing assay was used to determine the migration of HGFs and Western blotting was used to determine the expression of Hsp27 in them. Results The migration capability of HGFs was weakened with the increase of LPS concentration. Expression of Hsp27 was negative in normal HGFs, but was positive in the LPS-intervened HGFs with a concentration-dependent manner. Conclusion Hsp27 concentration increases in LPS-induced injury of HGFs, which suggests that Hsp27 plays an important role during the occurrence of LPS-induced injury of HGFs.

Key words: Human gingival fibroblasts, Heat shock protein 27, Epithelial injury, Lipopolysaccharide

CLC Number: 

  • R782.3
[1] Zhang P, Leu JI, Murphy ME, et al. Crystal structure of the stress-inducible human heat shock protein 70 substrate-binding domain in complex with peptide substrate[J]. PLoS One, 2014, 24, 9(7):e103518.
[2] Laplante AF, Moulin V, Auger FA, et al. Expression of heat shock proteins in mouse skin during wound healing[J]. J Histochem Cytochem, 1998, 46(11):1291-1301.
[3] Jain S, De la Cruz J, Kang E, et al. Expression of phosphorylated heat shock protein 27 during corneal epithelial wound healing[J]. Cornea, 2012, 31(7):820-827.
[4] Hirano S, Shelden EA, Gilmont RR. HSP27 regulates fibroblast adhesion, motility, and matrix contraction[J]. Cell Stress Chaperones, 2004, 9(1):29-37.
[5] 兰卫东,解保生.热休克蛋白27在大鼠牙髓炎中的免疫组化研究[J].昆明医学院学报, 2005, 26(3):28-32. LAN Weidong, XIE Baosheng. An experimental study on the dynamic expression of HSP27 in rats pulpitis[J]. Academic Journal of Kunming Medical College, 2005, 26(3):28-32.
[6] Jiang ZL, Cui YQ, Gao R, et al. Study of TNF-α, IL-1β and LPS levels in the gingival crevicular fluid of a rat model of diabetes mellitus and periodontitis[J]. Dis Markers, 2013, 34(5):295-304.
[7] Belibasakis GN. Microbiological and immuno-pathological aspects of peri-implant diseases[J]. Arch Oral Biol, 2014, 59(1):66-72.
[8] Heitz-Mayfield LJA, Lang NP. Comparative biology of chronic and aggressive periodontitis vs. peri-implantitis[J]. Periodontol 2000, 2010, 53:167-181.
[9] Padial-Molina M, Suarez F, Rios HF. Guidelines for the diagnosis and treatment of peri-implant diseases[J]. Int J Periodontics Restorative Dent, 2014, 34(6):e102-e111.
[10] Heitz-Mayfield LJ. Peri-implant diseases:diagnosis and risk indicators[J]. J Clin Periodontol, 2008, 35(8 Suppl):292-304.
[11] Malhotra R, Bird MI. L-selectin—a signalling receptor for lipopolysaccharide[J]. Chem Biol, 1997, 4(8):543-547.
[12] DeCarlo AA, Grenett HE, Harber GJ, et al. Induction of matrix metalloproteinases and a collagen‐degrading phenotype in fibroblasts and epithelial cells by secreted Porphyromonas gingivalis proteinase[J]. J Periodontal Res, 1998, 33(7):408-420.
[13] Ciocca DR, Oesterreich S, Chamness GC, et al. Biological and clinical implications of heat shock protein 27000 (Hsp27):a review[J]. J Natl Cancer Inst, 1993, 85(19):1558-1570.
[14] Liu J, Hong S, Feng Z, et al. Regulation of lipopolysaccharide-induced inflammatory response by heat shock protein 27 in THP-1 cells[J]. Cell Immunol, 2010, 264(2):127-134.
[15] 武文妍,王翔宇,孙克勤,等.脂多糖介导人牙周膜成纤维细胞损伤过程中热休克蛋白27表达的研究[J].中国药物与临床, 2011, 11(12):1381-1382. WU Wenyan, WANG Xiangyu, SUN Keqin, et al. Expression of HSP27 in lipopolysaccharide-induced injury of human periodontal ligament fibroblasts[J]. Chinese Remedies & Clinics, 2011, 11(12):1381-1382.
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