JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 32-35.doi: 10.6040/j.issn.1671-7554.0.2014.590

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Effects of PTH on gene ONC, OPN, C-FOS, COL1, VEGF, RUNX2 and ALP during the distraction osteogenesis in rat mandible

DU Tengfei1, LIU Guoming2, GUO Xiaomei3, Ching-Chang Ko3, SHANG Hongguo2   

  1. 1. Department of Stomtology, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Stomtology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    3. Department of Orthodontics, University of North Carolina, Chapel Hill, 27599-7454 North Carolina, USA
  • Received:2014-09-08 Revised:2015-01-22 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To explore the effects of parathyroid hormone (PTH) on gene ONC, OPN, C-FOS, COL1, VEGF, RUNX2 and ALP during the distraction osteogenesis (DO) in rat mandible. Methods A total of 30 DO models were established with male rats, which were then randomly divided into 5 groups, with 6 animals in each group. Group 1(DO, no PTH) was harvested 8 weeks after surgery, and the tissues were tested using HE and micro-CT. Group 2 (DO, no PTH), Group 3(DO, PTH), Group 4 (no DO, PTH), Group 5 (control Group, no DO, no PTH) were harvested 1 week after surgery, and Real-time PCR was used to quantify the genes:ONC, OPN, C-FOS, COL1, VEGF, RUNX2 and ALP. Results In Group 1, histological test and micro-CT showed new bone formation in the gap. In Groups 2, 3 and 4, especially in Group 3, RT-PCR test showed that compared with the control group, OPN, COL1, RUNX2 and ALP genes were significantly increased (P<0.05), while ONC , C-FOS, and VEGF genes were not significantly different (P>0.05). Conclusion Intermittent administration of PTH increases bone formation only in the distraction period. PTH has synergetic effect on gene expressions of OPN, COL1, RUNX2 and ALP.

Key words: Mandible, Distraction osteogenesis, Rats, Parathyroid hormone

CLC Number: 

  • R782.2+3
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