JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 21-26.doi: 10.6040/j.issn.1671-7554.0.2014.1002

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Expression profiles of long noncoding RNAs in rat myocardial hypertrophy induced by pressure overload

JIANG Lei, ZHANG Lei, LIANG Jiangjiu   

  1. Department of Cardiovascular Health Care No.1, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2014-12-29 Revised:2015-04-01 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To analyze the expressions of long noncoding RNA (lncRNA) in rat myocardial hypertrophy induced by abdominal aortic banding and in healthy rat hearts. Methods Rat models with myocardial hypertrophy induced by abdominal aortic banding were established. After 4 weeks, the echocardiographic data, left ventricular mass index and the myocyte cross-section were tested. Expressions of ANF and β-MHC mRNA were determined with quantitative real-time polymerase chain reaction (qRT-PCR). Differences of lncRNA expression profiles were inspected with lncRNA microarray and validated with qRT-PCR. Gene co-expression network was built with normalized signal intensity of specifically expressed lncRNAs and mRNAs. Results We identified 6 969 lncRNAs, among which 80 were significantly up-regulated and 172 down-regulated. The expressions of lncRNA MRAK134201 and X89963 verified by qRT-PCR and microarray data were consistent. The network constructed from lncRNA XR_008680 was co-expressed with 111 coding mRNAs. Conclusion Long noncoding RNAs are differentially expressed in rat cardiac hypertrophy models, indicating that lncRNAs might play a role in the pathogenesis of myocardial hypertrophy.

Key words: Pressure overload, LncRNA array, Rats, Myocardial hypertrophy, Long noncoding RNA

CLC Number: 

  • R541
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