JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 15-20.doi: 10.6040/j.issn.1671-7554.0.2014.852

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Acute hyperglycemia exacerbates myocardial ischemia/reperfusion injury by inhibiting aldehyde dehydrogenase 2 activity in rats

LI Minghua1,2, WANG Jiali1, XU Feng1, YUAN Qiuhuan1, LIU Baoshan1,2, PANG Jiaojiao1,2, ZHANG Yun2, CHEN Yuguo1   

  1. 1. Department of Emergency & Chest Pain Center, Qilu Hospital of Shandong University, Key Laboratory of Emergency and Critical Care Medicine of Shandong Province, Jinan 250012, Shandong, China;
    2. Key Laboratory of Cardiovascular Remodeling & Function Research, Chinese Ministry of Education & Chinese Ministry of Public Health, Jinan 250012, Shandong, China
  • Received:2014-11-21 Revised:2015-03-18 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To investigate the activity changes and actions of aldehyde dehydrogenase 2 (ALDH2) in myocardial ischemia/reperfusion injury exacerbated by acute hyperglycemia. Methods A total of 48 male Wistar rats were randomly divided into 4 groups: sham operation (SHAM) group, normal saline control (CON) group, highblood glucose (HG) group, and HG with Alda-1 administration (HG+Alda-1) group, with 12 animals in each group. The left anterior descending artery (LAD) was occluded for 30 minutes followed by 1 hour reperfusion to establish myocardial ischemia-reperfusion rat models. Acute hyperglycemia rat models were established via jugular vein injection of 50% glucose (3 g/kg) during the ischemia period. Blood glucose levels were maintained at 20-28 mmol/L throughout the experiment by administration of glucose with trace pumping[4 mL/(kg·h)] during ischemia and reperfusion period. The rats in CON group and HG+Alda-1 group were given normal saline (6 mL/kg). The rats in HG+Alda-1 group were given Alda-1 (8.5 mg/kg) with trace pumping during ischemia and reperfusion. After reperfusion, ALDH2 activity of heart was detected with colorimetric method, changes of myocardial tissue morphology were observed with HE staining, myocardial infarction size was determined with TTC staining, and myocardial cell apoptosis was tested with TUNEL method. Results Blood glucose level was significantly increased in HG group compared with that of CON group [(23.4±0.21) vs (5.8±0.21)mmol/L, P<0.01]. Compared with CON group, the activity of ALDH2 in HG group was markedly decreased [(69.1±5.16)% vs (87.0±4.30)%, P<0.05]. Myocardial infarct size of HG group was remarkably increased compared with the CON group [(38.2±3.30)% vs (26.8±2.53)%, P<0.05]. Compared with HG group, myocardial infarct size of HG+Alda-1 group was notedly decreased [(27.8±2.50)% vs (38.2±3.30)%, P<0.05]. Myocardial apoptosis index of HG group was significantly higher than that of CON group [(16.1±0.83)% vs (13.1±0.39)%, P<0.05]. Compared with HG group, myocardial apoptosis of HG+Alda-1 group was significantly reduced [(13.6±0.51)% vs (16.1±0.83)%, P<0.05]. Conclusion Acute hyperglycemia significantly increases myocardial infarct size and myocardial apoptosis induced by myocardial ischemia-reperfusion injury and reduced ALDH2 activity, while enhanced ALDH2 activity can markedly decrease myocardial infarct size and myocardial apoptosis during ischemia-reperfusion injury in acute hyperglycemia rats.

Key words: Acute hyperglycemia, Myocardial ischemia, Myocardial reperfusion injury, Aldehyde dehydrogenase 2, Rats

CLC Number: 

  • R541.4
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