JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 27-30.doi: 10.6040/j.issn.1671-7554.0.2014.998

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Effect of blood glucose on expressions of GLUT1 and GLUT4 mRNA in myocardial tissues of diabetic rats

HAN Yunfeng1, XU Hua2, LIU Qian3, SUN Hanchen1, XU Dandan1, HOU Weikai1, HUANG Qingxian4   

  1. 1. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Endocrinology, The Fifth People's Hospital of Jinan, Jinan 250022, Shandong, China;
    3. Department of Endocrinology, First People's Hospital of Taian, Taian 271000, Shandong, China;
    4. Department of Endocrinology, Qingdao Branch of Qilu Hospital, Shandong University, Qingdao 260012, Shandong, China
  • Received:2014-12-25 Revised:2015-04-02 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To evaluate the effect of blood glucose on expressions of GLUT1 and GLUT4 mRNA in diabetic rats. Methods A total of 50 healthy SD rats were randomly divided into normal control group (n=10, NC group), and diabetic model group (n=40, DM group). Of the 40 DM models, 36 were divided into 4 subgroups according to different levels of blood glucose: >16.7 mmol/L (A group), 14-16.7 mmol/L (B group), 10-14 mmol/L (C group), < 10 mmol/L (D group), with 9 animals in each group. Insulin glargine was injected into abdomen. Twelve weeks later, blood samples were collected to detect FBG and HbA1c. Myocardial tissues were collected to measure GLUT1 and GLUT4 mRNA with PT-PCR. Results Compared with the NC group, GLUT1 and GLUT4 mRNA expressions significantly reduced in all DM groups (P<0.05). After blood glucose level was controlled with insulinglargine, GLUT1 and GLUT4 mRNA expressions slightly escalated in all DM groups in the following order: D > C > B > A (P<0.05). The GLUT1 and GLUT4 mRNA expressions were negatively correlated with the level of glucose and HbA1c (P<0.05). Conclusion Chronic hyperglycemia down-regulates GLUT1 and GLUT4 mRNA expressions. This may be a protection against chronic high blood glucose mediated by GLUT1 and GLUT4.

Key words: Glucose transporter 4, Myocardial tissues, Hemoglobin A1c, Diabetes, Glucose transporter 1

CLC Number: 

  • R589.1
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