JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 36-40.doi: 10.6040/j.issn.1671-7554.0.2014.909

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Expression of Hsp27 in lipopolysaccharide-induced injury of human gingival fibroblasts

ZHU Zhenkun, ZHOU Qin, SUN Ruinan, XU Xin   

  1. School of Stomatology of Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, Shandong, China
  • Received:2014-12-08 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To observe the expression of heat shock protein 27 (Hsp27) in injury of human gingival fibroblasts (HGFs) induced by lipopolysaccharide (LPS) with different concentrations. Methods The 3rd-8th generations of cultured HGFs were treated with serially diluted LPS. Would healing assay was used to determine the migration of HGFs and Western blotting was used to determine the expression of Hsp27 in them. Results The migration capability of HGFs was weakened with the increase of LPS concentration. Expression of Hsp27 was negative in normal HGFs, but was positive in the LPS-intervened HGFs with a concentration-dependent manner. Conclusion Hsp27 concentration increases in LPS-induced injury of HGFs, which suggests that Hsp27 plays an important role during the occurrence of LPS-induced injury of HGFs.

Key words: Human gingival fibroblasts, Heat shock protein 27, Epithelial injury, Lipopolysaccharide

CLC Number: 

  • R782.3
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