JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 1-5.doi: 10.6040/j.issn.1671-7554.0.2014.943

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Expressions of MiR210 and stat3 following global ischemia in rats and the potential relationship among MiR210, stat3 and HIF-1α

FAN Shaohua1, ZHANG Yichao2, YUAN Xiaodong2, BI Jingwen1, ZHANG Yichen2, LI Yun2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Critical Care Medicine, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2014-12-11 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To study the expression of signal transducer and activator of transcription 3 (stat3) in hippocampus and the expression of MiR210 in cerebral cortex following global ischemia in rats, and to explore the potential relationship among MiR210, stat3 and hypoxia inducible factor-1α (HIF-1α). Methods Sixty-six SD rats were randomly divided into three groups:sham-operated group, global ischemia group (GI group), and stattic+global ischemia group (stattic+GI group). The global ischemia model was induced by modified four-vessel occlusion method. Stat3, p-stat3 and HIF-1α protein expressions in hippocampus were detected by Western blotting. MiR210 and HIF-1α mRNA expressions in cerebral cortex were detected by Real-time PCR. Results Compared with sham-operated group, stat3, p-stat3 and HIF-1α protein expressions in hippocampus and MiR210 and HIF-1α mRNA expressions in cerebral cortex of GI group significantly increased (all P<0.05). Compared with GI group, p-stat3 and HIF-1α protein expressions in hippocampus of stattic+ GI group significantly decreased, stat3 protein expression showed no statistical difference (P>0.05), and HIF-1α mRNA expression in cerebral cortex signifcantly decreased (P<0.05). Conclusion Expressions of MiR210 and stat3 have a marketable change after global ischemia reperfusion, and regulatory relationships may exist among stat3, MiR210 and HIF-1α.

Key words: Rat, MiR210, Signal transducer and activator of transcripation 3, Global ischemia, Hypoxia inducible factor-1α

CLC Number: 

  • R743.31
[1] Pulsinelli WA, Brierley JB, Plum F. Temporal profile of neuronal damage in a model of transient forebrain ischemia[J]. Ann Neurol, 1982, 11(5):491-498.
[2] Kirino T, Tamura A, Sano K. Delayed Neuronal Death in the rat hippocampus following transient forebrain ischemia[J]. Acta Neuropathol, 1984, 64(2):139-147.
[3] Dziennis S, Alkayed NJ. Role of signal transducer and activator of transcription 3 in neuronal survival and regeneration[J]. Rev Neurosci, 2008, 19(4-5):341-361.
[4] Kisseleva T, Bhattacharya S, Braunstein J, et al. Signaling through the JAK/STAT pathway, recent advances and future challenges[J]. Gene, 2002, 285(1-2):1-24.
[5] Chan SY, Loscalzo J. MicroRNA-210:a unique and pleiotropic hypoxamir[J]. Cell Cycle, 2010, 9(6):1072-1083.
[6] Zeng L, Liu J, Wang Y, et al. MicroRNA-210 as a novel blood biomarker in acute cerebral ischemia[J]. Front Biosci, 2011, 3(4):1265-1272.
[7] Satriotomo I, Bowen KK, Vemuganti R. JAK2 and STAT3 activation contributes to neuronal damage following transient focal cerebral ischemia[J]. J Neurochem, 2006, 98(5):1353-1368.
[8] Pulsinelli WA, Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat[J]. Stroke, 1979, 10(3):267-272.
[9] Schust J, Sperl B, Hollis A, et al. Stattic:a small-molecule inhibitor of STAT3 activation and dimerization[J]. Chem Biol, 2006, 13(11):1235-1242.
[10] Yadav A, Kalita A, Dhillon S, et al. JAK/STAT3 pathway is involved in survival of neurons in response to insulin-like growth factor and negatively regulated by suppressor of cytokine signaling-3[J]. J Biol Chem, 2005, 280(36):31830-31840.
[11] Suzuki S, Tanaka K, Nogawa S, et al. Phosphorylation of signal transducer and activator of transcription-3 (Stat3) after focal cerebral ischemia in rats[J]. Exp Neurol, 2001, 170(1):63-71.
[12] Wen TC, Peng H, Hata R, et al. Induction of phosphorylated-stat3 following focal cerebral ischemia in mice[J]. Neurosci Letters, 2001, 303(3):153-156.
[13] 谢慧芳,徐如祥,魏继鹏. JAK2/STAT3信号转导通路在缺血性脑损伤中的作用机制的研究[J].中风与神经疾病杂志, 2008, 25(2):135-138. XIE Huifang, XU Ruxiang, WEI Jipeng, et al. The mechanism of JAK2/STAT3 signal pathway in ischemic brain injury in rats[J]. Apoplexy and Nervous Disease, 2008, 25(2):135-138.
[14] Bergeron M, Yu YA, Solway KE, et al. Induction of hypoxia-induced factor-1(HIF-1) and its target genes following focal ischemia in rat brain[J]. Eur J Neurosci, 1999, 11(12):4159-4170.
[15] Lee JW, Bae SH, Jeong JW, et al. Hypoxia-inducible factor (HIF-1)α:its protein stability and biological functions[J]. Exp Mol Med, 2004, 36(1):1-12.
[16] Jung JE, Lee HG, Cho IH, et al. STAT3 is a potential modulator of HIF-1-mediated VEGF expression in human renal carcinoma cells[J]. FASEB, 2005, 19(10):1296-1298.
[17] Nechemia AY, Khamaisi M, Rosenberger C, et al. In vivo evidence suggesting reciprocal renal hypoxia-inducible factor-1 upregulation and signal transducer and activator of transcription3 activation in response to hypoxic and non-hypoxic stimuli[J]. Clin Exp Pharmacol Physiol, 2013, 40(4):262-272.
[18] Kulshreshtha R, Ferracin M, Wojcik SE, et al. A microRNA signature of hypoxia[J]. Mol Cell Biol, 2007, 27(5):1859-1867.
[19] Huang X, Le QT, Giaccia AJ. MiR-210--micromanager of the hypoxia pathway[J]. Trends Mol Med, 2010, 16(5):230-237.
[20] Chang KP, Lee HC, Huang SH, et al. MicroRNA signatures in ischemia-reperfusion injury[J]. Ann Plast Surg, 2012, 69(6):668-671.
[21] Jeyaseelan K, Lim KY, Armugam A. MicroRNA expression in the blood and brain of rats subjected to transient focal ischemia by middle cerebral artery occlusion[J]. Stroke, 2008, 39(3):959-966.
[22] Liu DZ, Tian Y, Ander BP, et al. Brain and blood microRNA expression profiling of ischemic stroke, intracerebral hemorrhage, and kainate seizures[J]. J Cereb Blood Flow metab, 2010, 30(1):92-101.
[23] Zeng L, He X, Wang Y, et al. MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain[J]. Gene Ther, 2014, 21(1):37-43.
[24] 高法梁,娄远蕾,阮琼芳, 等.缺血性脑损伤大鼠MicroRNA-210的动态变化[J].实验与检验医学,2010, 28(3):230-232. GAO Faliang, LOU Yuanlei, RUAN Qiongfang, et al. Dynamic changes of miR-210 after brain stroke in rats[J]. Experimental and Laboratory Medicine, 2010, 28(3):230-232.
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