JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (5): 43-48.doi: 10.6040/j.issn.1671-7554.0.2016.492

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Uniaxial-channel collagen scaffolds loaded with CBD-BDNF improves the recovery of rat spinal cord injury

CHEN Lei1,2, LIU Dongxiao1, LI Kaiming1, SONG Xinqiang1,2, ZENG Xiansi1,2, JIANG Lijie1   

  1. 1. College of Life Science, Xinyang Normal University, Xinyang 464000, Henan, China;
    2. Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, Henan, China
  • Received:2016-05-03 Online:2017-05-10 Published:2017-05-10

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Abstract: Objective To explore the efficacy of uniaxial guidance collagen scaffolds loaded with collagen binding domain-brain derived neurotrophic factor(CBD-BDNF)in the repair of rat spinal cord injury(SCI). Methods Rat hemisection SCI models were established and divided into 3 groups: control group, collagen group, and CBD-BDNF/collagen(CBC)group. Nerve fiber immunofluorescence staining, BBB score, and inclined plate test were adopted to evaluate the repair efficacy. Results Axons grew through collagen scaffolds in linear fashion in vitro. In animal experiments, CBC group displayed more regenerated neurofilament with linear direction than the other two groups(P<0.05). Nine weeks after implantation, the BBB score of CBC group reached 8(7, 13), significantly higher than the control group［1(1, 1)］ and collagen group［2(1, 2)］,(P<0.05). Inclined plane results also showed significant improvement of functional recovery after SCI in CBC group than the other two groups(P<0.05). Conclusion Collagen scaffolds loaded with CBD-BDNF significantly improves the SCI recovery evaluated by the BBB scale, inclined plane test and immunohistochemical staining with antineurofilament antibody, which suggests that uniaxial guidance 山 东 大 学 学 报 (医 学 版)55卷5期 -陈磊,等.有序胶原材料联合CBD-BDNF对大鼠脊髓损伤的修复作用 \=-collagen scaffolds loaded with CBD-BDNF may serve as an effective strategy for SCI repair.

Key words: Spinal cord injury, Collagen binding domain-brain derived neurotrophic factor, BBB score, Inclined plane score, Uniaxial guidance collagen scaffolds

CLC Number: 

  • R651.2
[1] Riblett BW, Francis NL, Wheatley MA, et al. Ice-templated scaffolds with microridged pores direct DRG neurite growth[J]. Adv Funct Mater, 2012, 22(23): 4920-4923.
[2] Saglam A, Perets A, Canver AC, et al. Angioneural crosstalk in scaffolds with oriented microchannels for regenerative spinal cord injury repair[J]. J Mol Neurosci, 2013, 49(2): 334-346.
[3] Ukegawa M, Bhatt K, Hirai T, et al. Bone marrow stromal cells combined with a honeycomb collagen sponge facilitate neurite elongation in vitro and neural restoration in the hemisected rat spinal cord[J]. Cell Transplant, 2014, 24(7): 1283-1297.
[4] Cholas RH, Hsu HP, Spector M. The reparative response to cross-linked collagen-based scaffolds in a rat spinal cord gap model[J]. Biomaterials, 2012, 33(7): 2050-2059.
[5] Weishaupt N, Blesch A, Fouad K. BDNF: the career of a multifaceted neurotrophin in spinal cord injury[J]. Exp Neurol, 2012, 238(2): 254-264.
[6] Smith PA. BDNF: no gain without pain?[J]. Neuroscience, 2014, 283: 107-123.
[7] Han Q, Sun W, Lin H, et al. Linear ordered collagen scaffolds loaded with collagen-binding brain-derived neurotrophic factor improve the recovery of spinal cord injury in rats[J]. Tissue Eng Part A, 2009, 15(10): 2927-2935.
[8] Li X, Xiao Z, Han J, et al. Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair[J]. Biomaterials, 2013, 34(21): 5107-5116.
[9] Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats[J]. J Neurotrauma, 1995, 12(1): 1-21.
[10] Kim M, Park SR, Choi BH. Biomaterial scaffolds used for the regeneration of spinal cord injury(SCI)[J]. Histol Histopathol, 2014, 29(11): 1395-1408.
[11] Haggerty AE, Oudega M. Biomaterials for spinal cord repair[J]. Neurosci Bull, 2013, 29(4): 445-459.
[12] Wang N, Zhang S, Zhang AF, et al. Sodium hyaluronate-CNTF gelatinous particles promote axonal growth, neurogenesis and functional recovery after spinal cord injury[J]. Spinal Cord, 2014, 52(7): 517-523.
[13] Davidenko N, Gibb T, Schuster C, et al. Biomimetic collagen scaffolds with anisotropic pore architecture[J]. Acta Biomater, 2012, 8(2): 667-676.
[14] Sridharan R, Reilly RB, Buckley CT. Decellularized grafts with axially aligned channels for peripheral nerve regeneration[J]. J Mech Behav Biomed Mater, 2015, 41: 124-135.
[15] Francis NL, Hunger PM, Donius AE, et al. An ice-templated, linearly aligned chitosan-alginate scaffold for neural tissue engineering[J]. J Biomed Mater Res A, 2013, 101(12): 3493-3503.
[16] Francis NL, Hunger PM, Donius AE, et al. Strategies for neurotrophin-3 and chondroitinase ABC release from freeze-cast chitosan-alginate nerve-guidance scaffolds[J]. J Tissue Eng Regen Med,2017,11(1)285-294.
[17] Stokols S, Tuszynski MH. Freeze-dried agarose scaffolds with uniaxial channels stimulate and guide linear axonal growth following spinal cord injury[J]. Biomaterials, 2006, 27(3): 443-451.
[18] Shi Q, Gao W, Han X, et al. Collagen scaffolds modified with collagen-binding bFGF promotes the neural regeneration in a rat hemisected spinal cord injury model[J]. Sci China Life Sci, 2014, 57(2): 232-240.
[19] Altinova H, Mollers S, Fuhrmann T, et al. Functional improvement following implantation of a microstructured, type-I collagen scaffold into experimental injuries of the adult rat spinal cord[J]. Brain Res, 2014, 1585: 37-50.
[20] 徐旭东. 多孔性壳聚糖-胶原材料促进神经纤维生长的实验研究[J]. 山东大学学报(医学版), 2011, 49(8): 45-47. XU Xudong. Chitosan-collagen biomaterial promotes the growth of neural fibers of the sciatic nerve[J]. Journal of Shandong University(Health Sciences), 2011, 49(8): 45-47.
[21] Autry AE,Monteggia LM. Brain-derived neurotrophic factor and neuropsychiatric disorders[J]. Pharmacol Rev, 2012, 64(2): 238-258.
[22] Han S, Wang B, Jin W, et al. The linear-ordered collagen scaffold-BDNF complex significantly promotes functional recovery after completely transected spinal cord injury in canine[J]. Biomaterials, 2015, 41: 89-96.
[23] Han Q, Jin W, Xiao Z, et al. The promotion of neural regeneration in an extreme rat spinal cord injury model using a collagen scaffold containing a collagen binding neuroprotective protein and an EGFR neutralizing antibody[J]. Biomaterials, 2010, 31(35): 9212-9220.
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