JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (5): 43-48.doi: 10.6040/j.issn.1671-7554.0.2016.492

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Uniaxial-channel collagen scaffolds loaded with CBD-BDNF improves the recovery of rat spinal cord injury

CHEN Lei1,2, LIU Dongxiao1, LI Kaiming1, SONG Xinqiang1,2, ZENG Xiansi1,2, JIANG Lijie1   

  1. 1. College of Life Science, Xinyang Normal University, Xinyang 464000, Henan, China;
    2. Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, Henan, China
  • Received:2016-05-03 Online:2017-05-10 Published:2017-05-10

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Abstract: Objective To explore the efficacy of uniaxial guidance collagen scaffolds loaded with collagen binding domain-brain derived neurotrophic factor(CBD-BDNF)in the repair of rat spinal cord injury(SCI). Methods Rat hemisection SCI models were established and divided into 3 groups: control group, collagen group, and CBD-BDNF/collagen(CBC)group. Nerve fiber immunofluorescence staining, BBB score, and inclined plate test were adopted to evaluate the repair efficacy. Results Axons grew through collagen scaffolds in linear fashion in vitro. In animal experiments, CBC group displayed more regenerated neurofilament with linear direction than the other two groups(P<0.05). Nine weeks after implantation, the BBB score of CBC group reached 8(7, 13), significantly higher than the control group［1(1, 1)］ and collagen group［2(1, 2)］,(P<0.05). Inclined plane results also showed significant improvement of functional recovery after SCI in CBC group than the other two groups(P<0.05). Conclusion Collagen scaffolds loaded with CBD-BDNF significantly improves the SCI recovery evaluated by the BBB scale, inclined plane test and immunohistochemical staining with antineurofilament antibody, which suggests that uniaxial guidance 山 东 大 学 学 报 (医 学 版)55卷5期 -陈磊,等.有序胶原材料联合CBD-BDNF对大鼠脊髓损伤的修复作用 \=-collagen scaffolds loaded with CBD-BDNF may serve as an effective strategy for SCI repair.

Key words: Spinal cord injury, Collagen binding domain-brain derived neurotrophic factor, BBB score, Inclined plane score, Uniaxial guidance collagen scaffolds

CLC Number: 

  • R651.2
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