Abstract: Objective  To explore the roles of CXCL16 and oxygenized low density lipoprotein (ox-LDL) level in mice model with adriamycin-induced nephropathy. Methods  Fifty male Balb/c mice were separated into the model group and the control group. Mice in the model group were intravenously injected with a single dose of adriamycin (10.5mg/kg), and those in the control group were injuected with the same dosage of physiological saline. Determined 24h urinary protein were observed at the end of the 0, 1st, 3rd, 5th and 7th weeks; serum and cholesterol were determined by automatic biochemical analyzer; serum CXCL16,  ox-LDL and urinary CXCL16 were determined by ELISA; the expressions of kidney CXCL16 and ox-LDL were detected by semiquantitative immunohistochemistry, and pathology were observed by light and electron microscopy. Results  Decreased serum albumin(P＜0.05) and increased proteinuria(P＜0.01) and serum cholesterol(P＜0.05) levels were observed in the model group compared with those in the control group. CXCL16 levels in serum, urine and renal tissue and ox-LDL levels in serum and renal tissue increased significantly in the model group, compared with those in the control group(P＜0.01). And it kept insistent increase for the model mice as time went on. CXCL16 and ox-LDL protein expressions in kidneys in the model group were strengthed remarkably, compared with those in the control group(P＜0.01). Conclusion  CXCL 16  and ox-LDL might take part in the progress of adriamycin-induced nephropathy and correlate with the severity of disease. Serum and urinary CXCL16 levels might be a non-invasive predictor to evaluate the severity of renal injury.

Key words: Nephropathy, Balb/c mice, CXCL16, Oxygenized low density lipoprotein, Adriamycin