JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 28-32.doi: 10.6040/j.issn.1671-7554.0.2014.853

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Impacts of Simvastatin on DNA methyltransferase of cell line NB4 in acute myeloid leukemia

YANG Juan1, QIU Zongjian2, SONG Qiang1   

  1. 1. Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Hematology, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350009, Fujian, China
  • Received:2014-11-21 Revised:2015-02-28 Online:2015-06-10 Published:2015-06-10

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Abstract: Objective To explore the impacts of Simvastatin (SIM) on DNA methyltransferase (DNMT) of cell line NB4 in acute myeloid leukemia (AML) and to investigate the possible mechanism. Methods NB4 cells were treated with different concentrations of SIM (final concentration 0, 5, 10 and 15 μmol/L). DNMT activities were detected by DNMT activity/inhibition assay. The expressions of DNMT1, DNMT3A and P53 mRNA were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The expression of DNMT1, DNMT3A and DNMT3B protein levels were analyzed by Western blotting. The apoptotic rate was measured by flow cytometry. Results The DNMT activity/inhibition assay showed a dose-dependent downregulation of DNMT activities after SIM treatment (P<0.05). The expression levels of DNMT1, DNMT3A mRNA and protein were downregulated while P53 mRNA were upregulated with the increase of SIM concentration (P<0.05). DNMT3B protein level did not change significantly (P>0.05). SIM induced NB4 cell apoptosis. Conclusion Simvastatin can downregulate the DNMT activities and expression levels in AML cell line NB4 and promote cell apopotosis, which may act as a potential DNA-hypomethylating agent for AML.

Key words: Demethylation, NB4 cell line, DNA methyltransferase, Simvastatin, Acute myeloid leukemia

CLC Number: 

  • R551.3
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