JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 24-29.doi: 10.6040/j.issn.1671-7554.0.2014.908

Previous Articles     Next Articles

Role of CXCL16 in mice with adriamycin induced nephropathy and the protective effects of simvastatin

WANG Cong1, SUN Shuzhen1, ZHEN Junhui2, LI Qian1, XU Yihuai1   

  1. 1. Department of Pediatric Nephrology and Rheumatism and Immunology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-12-07 Online:2015-09-10 Published:2015-09-10
  • Contact: 孙书珍。E-mail:ssztml@163.com E-mail:ssztml@163.com

PDF (PC)

329

Abstract: Objective To investigate the roles of chemokine ligand 16 (CXCL16) and oxidized low density lipoprotein (ox-LDL) in mice model with adriamycin induced nephropathy and their relationship with the renal tissue morphology, in order to explore the renal protective effects of simvastatin on adriamycin nephropathy. Methods We divided the mice into the following three groups: control group (n=5), adriamycin nephropathy group (n=5) and simvastatin treated adriamycin nephropathy group (n=5). Each group was treated with corresponding treatment respectively. 5 weeks after injection of adriamycin, the blood and 24 h urine were collected for the detection of general biochemical parameters and serum ox-LDL. After blood and 24 h urine collected, the mice were weighed and sacrificed with kidneys harvested. The renal morphology was observed by light microscopy and transmission electron microscopy, the expressions of CXCL16 and ox-LDL in podocytes were detected by double immunofluorescence techniques. Results Compared with the control group, the levels of TC and ox-LDL in the adriamycin nephropathy and simvastatin treated adriamycin nephropathy were significantly higher (P<0.05), the expressions of CXCL16 and ox-LDL in renal tissue were significantly increased (P<0.05). Compared with the adriamycin nephropathy group, the expressions of renal CXCL16 and ox-LDL in simvastatin treated adriamycin nephropathy group were markedly reduced (P<0.05), but there were no significant differences found in serum TC and ox-LDL between adriamycin nephropathy and simvastatin treated adriamycin nephropathy group. Conclusion Simvastatin has a protective effect on kidney in mice with adriamycin nephropathy. The mechanism might be related to the decreasing expression of CXCL16 in glomerular podocytes, followed by the decreasing endocytosis of ox-LDL in podocytes.

Key words: Nephropathy, Oxidized low density lipoprotein, Adriamycin, Simvastatin, Chemokine ligand 16

CLC Number: 

  • R726.9
[1] Mazzei LJ, García IM, Altamirano L, et al. Rosuvastatin preserves renal structure following unilateral ureteric obstruction in the neonatal rat[J]. Am J Nephrol, 2012, 35(2): 103-113.
[2] de Barros EP, Garcia-Pinto AB, Machado PY, et al. Rosuvastatin beneficially alters the glomerular structure of kidneys from spontaneously hypertensive rats (SHRs)[J]. J Mol Histol, 2011, 42(4): 323-331.
[3] Liu YZ, Liu M, Zhang YM, Kang L, et al. Protective effects of rosuvastatin in experimental renal failure rats via improved endothelial function[J]. Biol Res Nurs, 2013, 15(3): 356-364.
[4] Jasinska M, Owczarek J, Orszulak-Michalak D. Statins: a new insight into their mechanisms of action and consequent pleiotropic effects[J]. Pharmacol Rep, 2007, 59(5): 483-499.
[5] Wantanasiri P, Satirapoj B, Charoenpitakchai M, et al. Periostin: a novel tissue biomarker correlates with chronicity index and renal function in lupus nephritis patients[J]. Lupus. 2015 Jan 14. pii: 0961203314566634.[Epub ahead of print]
[6] Lydia A, Asanuma K, Nonaka K, et al. Effects of 22-oxa-calcitriol on podocyte injury in adrimycin-induced nephrosis[J]. Am J Nephrol, 2012, 35(1): 58-68.
[7] Saland JM, Pierce CB, Mitsnefes MM, et al. Dyslipidemia in children with chronic kidney disease[J]. Kidney Int, 2010, 78(11): 1154-1163.
[8] Lee HS, Song CY. Oxidized low-density lipoprotein and oxidative stress in the development of glomerulosclerosis[J]. Am J Nephrol, 2009, 29(3): 62-70.
[9] Zhen J, Li Q, Zhu Y, et al. Increased serum CXCL16 is highly correlated with blood lipids, urine protein and immune reaction in children with active nephrotic syndrome[J]. Diagn Pathol, 2014, 9:23. doi: 10.1186/1746-1596-9-23.
[10] 许艺怀, 孙书珍, 甄军晖, 等. CXCL16和oxLDL在阿霉素肾病小鼠中的变化及意义[J]. 山东大学学报: 医学版, 2014, 52(5): 68-72. XU Yihuai, SUN shuzhen, ZHEN Junhui, et al.Roles of CXCL16 and ox-LDL in mice with adriamycin induced nephropathy[J]. Journal of Shandong University:Health Sciences, 2014, 52(5): 68-72.
[11] Chen G, Lin SC, Chen J, et al. CXCL16 recruits bone marrow-derived fibroblast precursors in renal fibrosis[J]. J Am Soc Nephrol, 2011, 22(10):1876-1886.
[12] Xia Y, Entman ML, Wang Y. Critical role of CXCL16 in hypertensive kidney injury and fibrosis[J]. Hypertension, 2013, 62(6): 1129-1137.
[13] Gutwein P, Abdel-Bakky MS, Schramme A, et al. CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein[J]. Am J Pathol, 2009, 174(6): 2061-2072.
[14] Chen G, Lin SC, Chen J, et al. CXCL16 recruits bone marrow-derived fibroblast precursors in renal fibrosis[J]. J Am Soc Nephrol, 2011, 22(10): 1876-1886.
[15] Wang L, Sun S, Zhou A, et al. oxLDL-induced lipid accumulation in glomerular podocytes: role of IFN-gamma, CXCL16, and ADAM10[J]. Cell Biochem Biophys, 2014, 70(1): 529-538.
[1] GAO Xiu-Rui, ZANG Heng-Chang. In vitro release of compound niacin extended-release and simvastatin immediaterelease double-layer tablets [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 109-113.
[2] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7-14.
[3] SONG Yu, MAO Yang, LIU Qingmei, QIAO Lei, TIE Yuanyuan, LU Lu, ZHANG Lei. Effect of Tongxinluo on three-dimensional strain of atherosclerotic vulnerable plaque in rabbits [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 1-7.
[4] LIAN Kaili, LI Yan, ZHANG Na, JIN Guiyuan, LIU Suxia. TIPE3 decreases breast cancer cell sensitivity to adriamycin by upregulating P-gp protein expression [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 36-42.
[5] WANG Xue, LI Qian, WANG Li, SUN Shuzhen, MA Aihua. CXCL16 gene silencing alleviates injury of mouse podocytes treated with the oxidized low-density lipoprotein [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 16-21.
[6] CHEN Zhixin, WANG Ying, CAO Xinran, HEI Naihao, LI Junlong, DONG Bo, GUAN Guangju. Evaluation of the protective effect of AVE0991 on streptozotocin-induced diabetic nephropathy rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 29-33.
[7] YANG Juan, QIU Zongjian, SONG Qiang. Impacts of Simvastatin on DNA methyltransferase of cell line NB4 in acute myeloid leukemia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(6): 28-32.
[8] LIN Jinxiu, SUN Dongsheng, ZHENG Xiao, YIN Dechao, LI Ye, ZHANG Lei. Enhancement of tendon-bone healing by topical use of simvastatin after ACL reconstruction [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 27-31.
[9] HUANG Jing, XU Ying, LIU Yi, XIN Wei, ZHAO Xu, LIU Lei, WAN Qiang. Effects of down-regulating of acetyl CoA carboxylase 2 on intracellular lipid accumulation and epithelial mesenchymal transition in high glucose cultured human renal proximal tubular epithelial cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(7): 16-21.
[10] XU Yihuai1, SUN Shuzhen1, ZHEN Junhui2, LI Qian1, WANG Cong1. Roles of CXCL16 and ox-LDL in mice with adriamycin-induced nephropathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 68-72.
[11] ZHANG Jin-hang1, ZHANG Lu-wei1, LIU Xin-nong2, TIAN Jun1. Expression of Tribble3 in db/db mice  renal tissues and the possible role in renal fibrosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(2): 38-43.
[12] YANG Zhiying, LIU Xiangchun, GUAN Guangju. Expression changes of methionine sulfoxide reductase B1 in the kidneys of instreptozocin-induced diabetic mice and its relationship with oxidative stress [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 29-34.
[13] LIU Yan, LIU Cong-cong, SHI Dan-dan, SUN Xiao, LI Jie. Value of virtual touch tissue quantification in the diagnosis of chronic allograft nephropathy after renal transplantation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(8): 78-81.
[14] GONG Li-li1， HOU Sen2， REN Yu-bo3， LI Lian-qing1， WANG Jing1， ZHANG Ting-guo4. Clinical and pathological significances of the expressions of IgA and CD35 in chronic tonsillitis and IgAN patients’ tonsillae [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(7): 92-95.
[15] LIU Jun1, ZHU Tian-rui2, LI Xiao-hong2. Effects of simvastatin on RhoA/ROCK pathway and secretion of Aβ42 in β-secretase transferred HEK293 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 1-4.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd