Alport综合征iPSCs差异性表达新小核糖核苷酸的分析

doi:10.6040/j.issn.1671-7554.0.2015.307

Abstract

Abstract: Objective To investigate the expression profile of iPSCs of novel microRNAs between patients with Alport syndrome (AS) and normal controls (NC), and to analyze the target genes. Methods The expression profile of novel microRNAs was acquired from previously induced iPSCs, using high-throughput sequencing platform. After that, TargetScan software was adopted to predict target genes, which were then compared with reference genes, and the significantly encriched GO items and KEGG pathways were selected. Results A total of 49 differently expressed novel microRNAs were selected, 33 of which were up-regulated and 16 were down-regulated. GO enrichment analysis indicated that the target genes mainly enriched in biological regulation and metabolism; cell component and signal transduction; enzymic catalytic reaction; transporter activity. KEGG pathway analysis showed that the target genes mainly participated in metabolic pathways, purine metabolism, and cancer transcriptional regulation. Conclusion Novel microRNAs of iPSCs from AS and from NC are differently expressed, and the target genes mainly take part in molecular function, cellular component, and biological activities. Those differently expressed novel microRNAs and target genes may play an important role in the pathogenesis of AS.

Key words: Induced pluripotent stem cells, Gene ontology enrichment, microRNA, Alport syndrome, Kyoto encyclopedia of genes and genomes pathway

CLC Number:

R394-33

CHEN Wenbiao, YU Xiangqi, DAI Yong. Different expressions of novel microRNAs of iPSCs from Alport syndrome[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 80-85.

References

[1] 董程, 陈斯琦, 张焱琴, 等. X-连锁Alport综合征的听力表现与皮肤α5(Ⅳ)胶原表达的关系[J]. 中华耳科学杂志, 2014, 12(2): 275-279. DONG Cheng, CHEN Siqi, ZHANG Yanqin, et al. Relationship between hearing phenotype and collagen type IV α5 Chain expression in skin in X-linked alport syndrome[J]. Chinese Journal of Otology, 2014, 12(2): 275-279.
[2] 李玉峰, 卫敏江, 吴伟岚, 等. 儿童肾活检977例病理及临床分析[J]. 上海交通大学学报: 医学版, 2014, 36(3): 333-337. LI Yufeng, WEI Minjiang, WU Weilan, et al. Clinical and pathological analysis of 977 children with renal biopsy[J]. Journal of Shanghai Jiaotong University: Medical Science, 2014, 36(3): 333-337.
[3] 陈楠. 重视遗传性肾脏疾病的筛查与诊治[J]. 中国实用内科杂志, 2014, 34(3): 217-219. CHEN Nan. Emphasis on screening and treatment of hereditary kidney diseases[J]. Chin J Pract Intern Med, 2014, 34(3): 217-219.
[4] Shen J, Hung MC. Signaling-Mediated Regulation of MicroRNA Processing[J]. Cancer Res, 2015, 75(5): 783-791.
[5] Chen Y, Luo R, Xu Y, et al. Generation of systemic lupus erythematosus-specific induced pluripotent stem cells from urine[J]. Rheumatol Int, 2013, 33(8): 2127-2134.
[6] Li Y, Zhang Z, Liu F, et al. Performance comparison and evaluation of software tools for microRNA deep-sequencing data analysis[J]. Nucleic Acids Res, 2012, 40(10): 4298-4305.
[7] Femminella GD, Ferrara N, Rengo G, et al. The emerging role of microRNAs in Alzheimer's disease[J]. Front Physiol, 2015, 6: 40. doi: 10.3389/fphys.2015.00040. eCollection 2015.
[8] Nishiguchi T, Imanishi T, Akasaka T, et al. MicroRNAs and Cardiovascular Diseases[J]. Biomed Res Int, 2015, 2015: 682857. doi: 10.1155/2015/682857. Epub 2015 Feb 1.
[9] Zhang J, Yan YG, Wang C, et al. MicroRNAs in osteosarcoma[J]. Clin Chim Acta, 2015, 444: 9-17. doi: 10.1016/j.cca.2015.01.025. Epub 2015 Feb 4.
[10] 王勇, 厉泉, 陈善良, 等. microRNA-133对终末期扩张型心肌病心肌纤维化调控作用的研究[J]. 山东大学学报: 医学版, 2015, 53(5): 60-65. WANG Yong, LI Quan, CHEN Sanliang, et al. Effects of microRNA-133 on end-stage myocardial fibrosis of dilated cardiomyopathy[J]. Journal of Shandong University: Health Sciences, 2015, 53(5): 60-65.
[11] Lahmy R, Soleimani M, Sanati MH, et al. MiRNA-375 promotes beta pancreatic differentiation in human induced pluripotent stem (hiPS) cells[J]. Mol Biol Rep, 2014, 41(4): 2055-2066.
[12] Roese-Koerner B, Stappert L, Koch P, et al. Pluripotent stem cell-derived somatic stem cells as tool to study the role of microRNAs in early human neural development[J]. Curr Mol Med, 2013, 13(5): 707-722.
[13] Chen WB, Huang JR, Yu XQ, et al. Identification of microRNAs and their target genes in Alport syndrome using deep sequencing of iPSCs samples[J]. J Zhejiang Univ Sci B, 2015, 16(3): 235-250.
[14] Tan Kuibi, Chen Jing, Li Wuxian, et al. Genome-wide analysis of microRNAs expression profiling in patients with primary IgA nephropathy[J]. Genome, 2013, 56(3): 161-169.
[15] Chen WB, Lin XC, Tan KB, et al. Integrated profiling of microRNA expression in membranous nephropathy using high-throughput sequencing technology[J]. Int J Mol Med, 2014, 33(1): 25-34.
[16] Sui WG, Lin H, Peng WJ, et al. Molecular dysfunctions in acute rejection after renal transplantation revealed by integrated analysis of transcription factor, microRNA and long noncoding RNA[J]. Genomics, 2013, 102(4): 310-322.
[17] Yin XL, Zhou YM, Zou MS, et al. The clinical and pathological features of Alport syndrome in children[J]. J Clin Pediatr, 2013, 31(12): 1125-1128.
[18] Steenhard BM, Vanacore R, Friedman D, et al. Upregulated expression of integrin alpha1 in mesangial cells and integrin alpha3 and vimentin in podocytes of Col4a3-null (Alport) mice[J]. PLoS One, 2012, 7(12): e50745. doi: 10.1371/journal~pone.0050745.Epub 2012 Dec7.
[19] Abrahamson DR. Role of the podocyte (and glomerular endothelium) in building the GBM[J]. Semin Nephrol, 2012, 32(4): 342-349.
[20] Murea M. Advanced kidney failure and hyperuricemia[J]. Adv Chronic Kidney Dis, 2012, 19(6): 419-424.
[21] O'Connell S, Tuite N, Ryan MP, et al. Cyclosporine A-induced oxidative stress in human renal mesangial cells: a role for ERK 1/2 MAPK signaling[J]. Toxicol Sci, 2012, 126(1): 101-113.

Related Articles 14

[1]	JIA Yueyue, LIU Hongbin, LI Jingbo, LI Jing, ZHANG Jiangtao, SUN Mei, SHI Yuhua. Expression of microRNA-200b in granulosa cells of PCOS patients and its significance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 63-68.
[2]	LI Jingbo, LIU Hongbin, JIA Yueyue, WANG Ze, SUN Mei, SHI Yuhua. Expression and clinical significance of microRNA-183 in polycystic ovary syndrome with insulin resistance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 69-74.
[3]	JIANG Yunshan, TAN Hong, LI Xiaoyan, SU Li, ZHANG Guoming, ZHANG Hongming, MENG Nan. Effects of perindopril on the expression of plasma miR-423-5p and cardiac function in patients with heart failure [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 55-59.
[4]	XI Fuli, ZHANG Mei. Regulation of microRNA-34a on SH2B3 expression during cardiac fibrosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(2): 6-10.
[5]	ZHANG Xiaohui, YAN Lei, QI Shasha, LU Zhenzhen, LI Mingjiang, ZHAO Xingbo. Occurrence of epithelia-mesenchymal transition in endometrial adenocarci-noma and the roles of miR200a/ZEB1 signaling pathway in this process [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(7): 48-52.
[6]	WANG Yong, LI Quan, CHEN Shanliang, WANG Dong, YU Jianmin, LI Min, LIU Tianqi. Effects of microRNA-133 on end-stage myocardial fibrosis of dilated cardiomyopathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 60-65.
[7]	CHENG Li, ZHANG Chuanbin, LI Meng, ZHANG Yuzhen, SUN Jinlong. Anti-fibrosis role of miR-29c in rat leptomeninge after experimental subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 22-25.
[8]	LIU Yimin, DU Lutao, WANG Lili, JIANG Xiumei, LI Juan, QU Ailin, WANG Haiyan, ZHENG Guixi, ZHANG Xin,YANG Yongmei, WANG Chuanxin. Identification and validation of suitable reference genes for the investigation of serum microRNA in bladder cancer patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 86-91.
[9]	LIU Lu, LIU Chuan-fang, WANG Min, TIAN Tian, YU Shuang, HE Na, SUN Yuan-xin, MA Dao-xin. The aberrant expression of microRNA-181a, microRNA-146a, microRNA-326 and microRNA-142-3p in peripheral blood of patients with immune thrombocytopenia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(12): 55-60.
[10]	FAN Zhi-bin1， YU Wei-fang2, ZHANG Li-jing1， LIU Bo1， WU Chen-peng3，ZHANG Xue-ming4， ZHAO Zeng-ren1. Expression of microRNA-150 in colorectal carcinoma and its clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(11): 85-89.
[11]	YU Yang1， ZHAO Jian2， ZHONG Kai-ze2， LIU Chun-yan1， LI Yi3， SHI Jing3， CHEN Wei-wen1， JIANG An-li1. Construction of eukaryotic expression vector for human miR-147a and its effects on the proliferation and invasion of lung cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(12): 25-30.
[12]	WANG Shu-jun， GENG Qin， ZHANG Xiu-ying, ZHANG Hong-ju, WEI Qiao-feng, CUI Yan-hui. Regulatory role of the let-7/miR-98 family in the IL-6 expression [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(1): 62-65.
[13]	LIANG Jing1, ZHANG Guiyu1, MA Benhong1, QIN Xuying1, LI Yue2, ZHAO Meng1. Differential expressions of microRNAs in endometrioid endometrial adenocarcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(8): 90-95.
[14]	KONG Lei， HAN Wen-xia， JIANG Xiu-yun， XU Jin， ZHANG Xu， LI Qiu， GUAN Qing-bo， GAO Ling， ZHAO Jia-jun. Expression and clinical significance of peripheral miR-34a during the onset of type 2 diabetes [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(10): 1-3.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Different expressions of novel microRNAs of iPSCs from Alport syndrome

PDF (PC)