JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (2): 6-10.doi: 10.6040/j.issn.1671-7554.0.2015.411

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Regulation of microRNA-34a on SH2B3 expression during cardiac fibrosis

XI Fuli, ZHANG Mei   

  1. Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-04-23 Online:2016-02-10 Published:2016-02-10

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Abstract: Objective To explore the pathological mechanism of abnormal SH2B3 expression during cardic fibrosis. Methods After H2C9 cells were treated with transformin growth factor-β1(TGF-β1)at different concentrations(10, 50, and 100 ng/mL)and hypoxia stimulation of various duration(3, 6, 12, 24 and 48 h), the expression of SN2B3 protein was detected with Western blotting, and the SN2B3 mRNA and miR-34a expressions were determined with qRT-PCR. After H2C9 cells were transfected with miR-34a mimic/inhibitor, the SH2B3 expression was examined with Western blotting. The effect of miR-34a on the activity of SH2B3 3'UTR was detected with luciferase activity assay. Results After treatment of TGF-β1 and hypoxia, SH2B3 expression decreased at both mRNA and protein levels(P<0.05), but miR-34a expression increased(P<0.05). The miR-34a could regulate SH2B3 expression by directly binding to 3'UTR(P<0.05). Conclusion SH2B3 participates in the fibrosis process of fibroblasts under the regulation of miR-34a.

Key words: Hypoxia, Cardiac fibrosis, Transformin growth factor-β1, MicroRNA

CLC Number: 

  • R542
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