JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 22-25.doi: 10.6040/j.issn.1671-7554.0.2014.771

Previous Articles     Next Articles

Anti-fibrosis role of miR-29c in rat leptomeninge after experimental subarachnoid hemorrhage

CHENG Li1, ZHANG Chuanbin1, LI Meng2, ZHANG Yuzhen2, SUN Jinlong2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Neurosurgery, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2014-10-31 Revised:2015-03-09 Online:2015-04-10 Published:2015-04-10

PDF (PC)

165

Abstract: Objective To explore the role of miR-29c in rat leptomeningeal fibrosis after subarachnoid hemorrhage (SAH). Methods A total of 26 healthy Sprague-Dawley (SD) rats were randomly divided into sham group (n=6), empty lentiviral group (n=8), and LV-rno-miRNA-29c group (n=12). 5 μL saline, empty lentivirus and LV-rno-miRNA-29c were respectively injected into the lateral cerebral ventricle of rats in each group. The SAH models were induced by injecting autologous blood twice into the cisterna magna, and 21 days later the rats were killed. Realtime PCR was used to detect the expressions of TGF-β1 and miR-29c. Masson staining was performed to observe collagen fiber. ELISA was adopted to measure the level of PICP in CSF. Results Increased TGF-β1 and decreased miR-29c were detected. The upregulation of miR-29c could significantly reduce the expressions of PICP in CSF and collagen in leptomeninge. Conclusion High expression of miR-29c may have potential therapeutic effects for leptomeningeal fibrosis by inhibiting collagen synthesis after SAH.

Key words: Allergic diseases, Allergens, Subarachnoid hemorrhage, Hydrocephalus, Leptomeningeal fibrosis, Transforming growth factor β1, microRNAs, Skin prick test

CLC Number: 

  • R651.1
[1] Sajanti J, Heikkinen E, Majamaa K. Transient increase in procollagen propeptides in the CSF after subarachnoid hemorrhage[J]. Neurology, 2000, 55(3): 359-363.
[2] Zhang Y, Huang XR, Wei LH, et al. MiR-29b as a therapeutic agent for angiotensin II-induced cardiac fibrosis by targeting TGF-beta/Smad3 signaling[J]. Mol Ther, 2014, 22(5): 974-985.
[3] Tiao MM, Wang FS, Huang LT, et al. MicroRNA-29a protects against acute liver injury in a mouse model of obstructive jaundice via inhibition of the extrinsic apoptosis pathway[J]. Apoptosis, 2014, 19(1): 30-41.
[4] Yang T, Liang Y, Lin Q, et al. MiR-29 mediates TGFbeta1-induced extracellular matrix synthesis through activation of PI3K-AKT pathway in human lung fibroblasts[J]. J Cell Biochem, 2013, 114(6): 1336-1342.
[5] Ramdas V, Mcbride M, Denby L, et al. Canonical transforming growth factor-beta signaling regulates disintegrin metalloprotease expression in experimental renal fibrosis via miR-29[J]. Am J Pathol, 2013, 183(6): 1885-1896.
[6] 张天一,刘飞,廖达光,等. 实验性蛛网膜下腔出血后柔脑膜纤维化的研究[J]. 中风与神经疾病杂志, 2008, 25(3): 277-280. ZHANG Tianyi, LIU Fei, LIAO Daguang, et al. Index of leptomeningeal fibrosis after experimental subarachnoid hemorrhage[J]. Chinese Journal of Critical Care Medicine, 2008, 25(3): 277-280.
[7] Flood C, Akinwunmi J, Lagord C, et al. Transforming growth factor-beta1 in the cerebrospinal fluid of patients with subarachnoid hemorrhage: titers derived from exogenous and endogenous sources[J]. J Cereb Blood Flow Metab, 2001, 21(2): 157-162.
[8] Moinuddin SM, Tada T. Study of cerebrospinal fluid flow dynamics in TGF-beta1 induced chronic hydrocephalic mice[J]. Neurol Res, 2000, 22(2): 215-222.
[9] Botfield H, Gonzalez AM, Abdullah O, et al. Decorin prevents the development of juvenile communicating hydrocephalus[J]. Brain, 2013, 136(Pt 9): 2842-2858.
[10] Lee P, Monaco ER, Friedlander RM. Blocking TGF-beta activity and associated inflammation may halt hydrocephalus[J]. Neurosurgery, 2013, 73(6): N13-N14.
[11] Makwana M, Jones LL, Cuthill D, et al. Endogenous transforming growth factor beta 1 suppresses inflammation and promotes survival in adult CNS[J]. J Neurosci, 2007, 27(42): 11201-11213.
[12] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116(2): 281-297.
[13] van Rooij E, Sutherland LB, Thatcher JE, et al. Dysregulation of microRNAs after myocardial infarction reveals a role of miR-29 in cardiac fibrosis[J]. Proc Natl Acad Sci U S A, 2008, 105(35): 13027-13032.
[14] Pandit KV, Milosevic J, Kaminski N. MicroRNAs in idiopathic pulmonary fibrosis[J]. Transl Res, 2011, 157(4): 191-199.
[15] Maurer B, Stanczyk J, Jungel A, et al. MicroRNA-29, a key regulator of collagen expression in systemic sclerosis[J]. Arthritis Rheum, 2010, 62(6): 1733-1743.
[16] Bian EB, Li J, Zhao B. MiR-29, a potential therapeutic target for liver fibrosis[J]. Gene, 2014, 544(2): 259-260.
[17] Qin W, Chung AC, Huang XR, et al. TGF-beta/Smad3 signaling promotes renal fibrosis by inhibiting miR-29[J]. J Am Soc Nephrol, 2011, 22(8): 1462-1474.
[18] Wang B, Komers R, Carew R, et al. Suppression of microRNA-29 expression by TGF-beta1 promotes collagen expression and renal fibrosis[J]. J Am Soc Nephrol, 2012, 23(2): 252-265.
[1] WANG Moulong, CHEN Yongkun, XING Deguang, QU Liangsuo, WANG Chengwei. A clinical analysis of transcranial Doppler in predicting delayed cerebral ischemia after ruptured aneurysm surgery [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 38-41.
[2] REN Baoxin, MA Yunfeng, LIU Dianwei, LI Zhuo, JIANG Yong. Effects of Wnt3a on neuronal autophagy and apoptosis in early brain injury after subarachnoid hemorrhage in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 11-15.
[3] ZHAO Hui-ling, DU Xi-xia, ZHANG Hai-rong, LIU Jian-hua, YANG Hui-rong. Analysis of characteristics of allergen skin prick test in children with allergic disease in Shijiazhuang region [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(Z1): 150-151.
[4] LI Zhuo1, JIANG Huili2, LIU Dianwei2, XIE Jie1, JIANG Yong2. Effects of Wnt3a on the neurogenesis in rats during early brain  injury after subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 15-19.
[5] MENG Fan-liang1， MENG Lei2, PENG Hong-hai2, SHI Jian-guo2, LIU Dian-wei2, JIANG Yong2. Effects of interferon-β on cardiopulmonary function after
subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 32-35.
[6] ZHAO Yong-hui1, Gao Jun2, LI Zhuo1, JIANG Yong2. Protective effects of progesterone on early brain injury in
rats after subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 55-.
[7] ZHENG Nan1,2, JI Yong3, SUN Ruo-peng1. Expressions of ICAM-1, NF-κB and p38MAPK
in the hippocampus of rabbits after SAH [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(10): 28-32.
[8] WANG Wen-ting, SHAN Fei, WANG Xiao-chuan, LIU Zhen-zhong, ZHANG Ji-xun, JIANG Du-yin. Effects of TGF-β1 on wound healing in diabetic rats  with seconddegree scald [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 19-23.
[9] GUO Jin-hui1, JIANG Yong2. Effect of Xingnaojing on apoptosis of hippocampal neurons in  rats with  subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 57-60.
[10] JI Yong, WANG Zhi-gang, WANG Cheng-wei, ZHANG Qing-lin . Expressions of p38MAPK, NF-κB, ICAM-1 after SAH and  their relations with the cause of CVS  [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 65-.
[11] LIU Dianwei1, JIANG Yong1, HAN Xiangyang2. Expression and significance of caspase-3, bax and bcl-2 in the hypothalamus of rats after experimental subarachnoid hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(2): 44-47.
[12] WANG Lijiang, DUAN Chuanzhi, HE Xuying, WEN Jun, LI Xifeng, LI Tielin,WANG Jianqi. Prognosis of subarachnoid hemorrhage due to small ruptured intracranial aneurysms treated by Guglielmi detachable coils endovascular embolization [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(2): 94-.
[13] JI Yong, WANG Zhi-gang, WANG Cheng-wei, ZHANG Qing-lin . Expression of p38MAPK, NF-κB and ICAM-1 in the basilar artery of rabbits after SAH [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(10): 44-47.
[14] WANG Ning, ZHANG Kejun, WANG Binquan, CHENG Nasha, LIU Taiqing. A survey of the relationship between the type and content of autumn airborne pollen and floors in Taiyuan [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(1): 67-69.
[15] SUN Jin-long,ZHANG Qing-lin,XI Guang-ming,SHEN Shui-jun,LI Wei-guo,SONG Qian. Aparoscope or／and ventriculoscope in the treatment of hydrocephalus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(6): 561-564.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd