JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (5): 13-18.doi: 10.6040/j.issn.1671-7554.0.2016.1378

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Pioglitazone ameliorates cardiac fibrosis induced by hypertension via regulating Sirt3

YAN Fangying, SHAN Xiaolan, LI Jingyuan, ZHANG Jie, YAN Xuefang, YANG Yi, BU Peili   

  1. Department of Cardiology, Qilu Hospital of Shangdong University, Jinan 250012, Shandong, China
  • Received:2016-10-25 Online:2017-05-10 Published:2017-05-10

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Abstract: Objective To investigate the effect of pioglitazone on cardiac fibrosis induced by hypertension and the possible mechanism. Methods Cardiac fibroblasts were extracted from newly-born SD rats and divided into 6 groups: negative control group, angiotension II(ANGII 1 μmol/L)group, pioglitazone(Piog 10 μmol/L)group, Piog+ANGII group, Sirt3.siRNA+ANGII group, and Sirt3.siRNA+Piog+ANGII group. The protein expressions of a-SMA, Sirt3, COL I and β-catenin were detected with Western blotting. The mRNA expressions of a-SMA, Sirt3, COL I and β-catenin were detected with quantitative real time PCR. The expression of COL I was evaluated with immuofluorescence staining. Results Compared with the negative control group, the ANGII group showed increased mRNA and protein expressions of a-SMA, COL I and β-catenin, decreased expression of Sirt3, enhanced green fluorescence of COL I, and severer cardiac fibrosis(all P<0.05). Compared with the ANGII group, the Piog+ANGII group 山 东 大 学 学 报 (医 学 版)55卷5期 -严芳英,等.吡格列酮通过调控Sirt3改善高血压引起的心肌纤维化机制 \=-showed increased mRNA and protein expressions of Sirt3, decreased expressions of a-SMA, COL I and β-catenin, lower green fluorescence intensity of COL I, and improved cardiac fibrosis(all P<0.05). Compared with the Piog+ANGII group, the Sirt3.siRNA+Piog+ANGII group showed increased mRNA and protein levels of a-SMA, COL I and β-catenin, decreased levels of Sirt3, and higher green fluorescence intensity of COL I(all P<0.05). However, there were no significant differences in the mRNA and protein expressions between Piog and Piog+ANGII groups(P>0.05), and between Sirt3.siRNA+ANGII and Sirt3. siRNA+Piog+ANGII groups(P>0.05). Conclusion Pioglitazone can improve cardiac fibrosis induced by hypertension via activation of Sirt3 and inhibition of β-catenin.

Key words: Pioglitazone, Hypertension, Cardiac fibrosis, β-catenin, Sirtuin3

CLC Number: 

  • R543.1
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