JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (8): 1-4.doi: 10.6040/j.issn.1671-7554.0.2014.905

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Effect of Lobelia alkaloids on endothelin-1 signal pathway in a rat model of pulmonary arterial hypertension

LIU Huimin1, LIU Deng2, LI Xiaoyu2, ZOU Shufeng2, JIANG Limin2, LI Yuhuan2   

  1. 1. Department of Physiology, School of Basic Medicine;
    2. School of Acupuncture and Massage, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China
  • Received:2014-12-05 Online:2015-08-10 Published:2015-08-10

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Abstract: Objective To observe the effect of Lobelia alkaloids on pulmonary vascular remodeling of pulmonary arterial hypertension (PAH) rat model and to explore its mechanism. Methods Twenty-four male Wistar rats were randomly divided into normal control group, model group and Lobelia alkaloids group. PAH rat models were established by giving monocrotaline (MCT) intraperitoneally, and the rats were given Lobelia alkaloids by intragastric administration for 4 weeks. HE staining, ELISA and immunohistochemical methods were used to study pulmonary morphological changes, endothelin-1 (ET-1) level in plasma and endothelin receptor A (ETA) expression in lung tissue. Results Lobelia alkaloids could significantly alleviate pulmonary artery remodeling and decrease the ET-1 level in plasma and ETA protein expression in lung tissue of PAH rat model. Conclusion Lobelia alkaloids can inhibit significantly pulmonary artery remodeling of PAH rat model induced by MCT which may be related to the decreased ET-1 level in plasma and ETA protein expression in lung tissue.

Key words: Pulmonary vascular remodeling, Endothelin-1, Lobelia alkaloids, Pulmonary arterial hypertension, Endothelin receptor A

CLC Number: 

  • R543.2
[1] Seferian A, Simonneau G. Therapies for pulmonary arterial hypertension: where are we today, where do we go tomorrow?[J]. Eur Respir Rev, 2013, 22(129): 217-226.
[2] 田丹,周达新,吕迁洲. 肺动脉高压的药物治疗[J]. 中国新药与临床杂志, 2013, 32(3): 181-185. TIAN Dan, ZHOU Daxin, LV Qianzhou. Drug therapy for pulmonary arterial hypertension[J]. Chin J New Drugs Clin Rem, 2013, 32(3): 181-185.
[3] Buckley MS, Staib RL, Wicks LM. Combination therapy in the management of pulmonary arterial hypertension[J]. Int J Clin Pract Suppl, 2013, 67(179): 13-23.
[4] 王婧婧,范秀珍,刘尚明,等.半边莲生物碱抑制内皮素-1诱导的人脐动脉平滑肌细胞增殖[J].中国病理生理杂志, 2006, 22(1): 26-30. WANG Jingjing, FAN Xiuzhen, LIU Shangming, et al. Inhibitory effects of Lobelia Chinensis Lour alkaloid on the proliferation of human umbilical artery vascular smooth muscle induced by endothelin-1[J]. Chinese Journal of Pathophysiology, 2006, 22(1): 26-30.
[5] 林雪群,万丽丹,唐迅,等.半边莲生物碱对高血压鼠脑基底动脉血管平滑肌细胞增殖的影响[J].解剖学杂志, 2011, 34(2): 188-190. LIN Xuequn, WAN Lidan, TANG Xun, et al. Effect of lobelia chinensis lour alkaloid on smooth muscle cell proliferation of cerebral basilar artery in hypertensive rat[J]. Chinese Journal of Anatomy, 2011, 34(2): 188-190.
[6] Guignabert C, Tu L, Girerd B, et al. New molecular targets of pulmonary vascular remodeling in pulmonary arterial hypertension: importance of endothelial communication[J]. Chest, 2015, 147(2): 529-537.
[7] 林梅,王一彪,苏宏,等. Smad信号通路及CTGF在依那普利抑制高肺血流性肺动脉高压形成中的作用机制[J]. 山东大学学报:医学版, 2011, 49(9): 16-20. LIN Mei, WANG Yibiao, SU Hong, et al. Mechanism of the Smad signaling pathway and connective tissue growth factor in the inhibition of pulmonary hypertension in rats by enalapril[J]. Journal of Shandong University: Health Sciences, 2011, 49(9): 16-20.
[8] 李彦川,李欣欣.几种常用的肺动脉高压动物模型简述[J].医学综述, 2012, 18(1): 121-123. LI Yanchuan, LI Xinxin. Brief introduction of common animal models for pulmonary hypertensioin[J]. Medical Recapitulate, 2012, 18(1): 121-123.
[9] Maarman G, Lecour S, Butrous G, et al. A comprehensive review: the evolution of animal models in pulmonary hypertension research; are we there yet?[J]. Pulm Circ, 2013, 3(4): 739-756.
[10] Lai YC, Potoka KC, Champion HC, et al. Pulmonary arterial hypertension: the clinical syndrome[J]. Circ Res, 2014, 115(1): 115-130.
[11] Shimoda LA, Laurie SS. Vascular remodeling in pulmonary hypertension[J]. J Mol Med (Berl), 2013, 91(3): 297-309.
[12] Chester AH, Yacoub MH. The role of endothelin-1 in pulmonary arterial hypertension[J]. Glob Cardiol Sci Pract, 2014, 29(2): 62-78.
[13] Li H, Chen SJ, Chen YF, et al. Enhanced endothelin-1 and endothelin receptor gene expression in chronic hypoxia[J]. J Appl Physiol, 1994, 77(3): 1451-1459.
[14] Lim KA, Kim KC, Cho MS, et al. Gene expression of endothelin-1 and endothelin receptor a on monocrotaline-induced pulmonary hypertension in rats after bosentan treatment[J]. Korean Circ J, 2010, 40(9): 459-464.
[15] 王婧婧,范秀珍,刘尚明,等.半边莲生物碱抑制内皮素诱导的大鼠主动脉平滑肌细胞增殖[J].中国动脉硬化杂志, 2006, 14(2): 107-110. WANG Jingjing, FAN Xiuzhen, LIU Shangming, et al. Lobelia chinensis lour alkaloids inhibiting rat aortic vascular smooth muscle cell proliferation induced by endothelin[J]. Chinese Journal of Arteriosclerosis, 2006, 14(2): 107-110.
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