JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (8): 5-10.doi: 10.6040/j.issn.1671-7554.0.2015.266

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Effects of 1,25(OH)2D3 on the induction of immune tolerance in Lewis rats with EAMG

LI Xiuhua1, LI Xiaoli1, DUAN Ruisheng1, ZHU Meijia1, CAO Lili2, LI Yanbin1, WANG Si1, YUE Longtao2, MA Qinghai3, LIU Fei1   

  1. 1. Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    2. Central Laboratory, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    3. Department of Laboratory, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-03-11 Online:2015-08-10 Published:2015-08-10

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Abstract: Objective To investigate the effects of 1,25(OH)2D3 on the induction of immune tolerance in Lewis rats with experimental autoimmune myasthenia gravis (EAMG). Methods EAMG models were established by subcutaneous injection of synthetic peptide R97-116 into both hind footpads of Lewis rats. The models were then divided into 1,25(OH)2D3 treatment group (experimental group) and control group. The clinical symptoms were evaluated. The expressions of CD80 and CD86 on MNC and Treg cells of lymph node mononuclear cells (MNC) were determined with flow cytometry. Cell proliferation was analyzed with CCK-8 test. Cell cycle was detected with flow cytometry. Cyto-kines of MNC supernatants were determined with ELISA. Serum anti-R97-116 IgG antibody was detected with ELISA. Results Compared with the control group, the experimental group exhibited lower clinical scores 28 d after first immunization (P <0.01) and less loss of body weight 35 d after first immunization (P <0.01); lower levels of CD80 and CD86 on MNC (P <0.05); higher percentages of CD4+CD25+ T cells and CD4+Foxp3+ T cells among lymph node MNC (P <0.01, P< 0.05); decreased lymphocyte proliferation in the presence of R97-116 antigen (P< 0.01); lower percentage of cells of S phase and of S phase plus G2/M phase (both P <0.05); higher level of IL-4 and lower levels of IFN-γ as well as IL-17 (all P <0.05); lower level of anti-rat R97-116 IgG antibody (P <0.05). Conclusion 1,25(OH)2D3 could ameliorate EAMG by down-regulating the expressions of CD80 and CD86 on MNC, up-regulating the number and function of Treg cells, inhibiting lymphocyte proliferation, changing the lymphocyte cell cycle, shifting cytokine profile from Th1/Th17 to Th2 type cytokines, and decreasing level of anti-R97-116 IgG antibody in serum.

Key words: Experimental autoimmune myasthenia gravis, Treg cells, Cytokines, 1,25(OH)2D3, Anti-R97-116 IgG, Mononuclear cells

CLC Number: 

  • R746.103
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