JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 25-28.doi: 10.6040/j.issn.1671-7554.0.2014.144

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Effects of azithromycin and dexamethasone on the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia

WU Fengjuan1, LI Tao2, QI Qian3, LI Yu2   

  1. 1. Department of Pulmonary Disease, Heze Municipal Hospital, Heze 274007, Shandong, China;
    2. Department of Pulmonary Disease, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-03-17 Revised:2014-06-10 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To evaluate the effect of azithromycin and dexamethasone on the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia. Methods The concentrations of ICAM-1 and TNF-α were detected by ELISA when A549 cells were cultured alone and were cultured with Aspergillus fumigatus conidia for 4 and 24 hours. The normal cultured A549 cells were taken as control group. After being cultured with conidia for 24 hours, A549 cells were treatd with azithromycin (0.5, 2.5 mg/L) or dexamethasone (0.5, 2.5 mg/L) and then the ICAM-1 and TNF-α concentrations were detected by ELISA. Results After being cultured with Aspergillus fumigatus conidia for 4 and 24 hours, both ICAM-1 and TNF-α secreted in A549 cells significantly increased(P<0.05), compared with control group. Compared with the stimulation of Aspergillus fumigatus conidia alone, 2.5 mg/L azithromycin significantly inhibited the secretion of TNF-α in A549 cells incubated with Aspergillus fumigatus conidia for 24 hours(P<0.05). While, 2.5 mg/L dexamethasone significantly inhibited the secretion of both TNF-α (P<0.05) and ICAM-1 in A549 cells incubated with Aspergillus fumigatus conidia for 24 hours (P<0.05). Conclusion Azithromycin and dexamethasone can inhibit the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia and thus relieve the inflammatory response of lung.

Key words: Cytokines, Azithromycin, Dexamethasone, Aspergillus fumigatus conidia, A549 cells

CLC Number: 

  • R379.6
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