JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 44-47.doi: 10.6040/j.issn.1671-7554.0.2014.217

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Significance and effect of emodin on activation of nuclear factor-κB in corneas of keratitis rats

CHEN Guoling1, REN Ran2, ZHANG Yinghui3, SONG Wei1, KAO Xin1, ZHANG Han1   

  1. 1. Department of Ophthalmology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Radiology, the 5th People's Hospital of Jinan, Jinan 250022, Shandong, China;
    3. Department of Medical Records, the Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2014-04-12 Revised:2014-06-06 Online:2014-09-10 Published:2014-09-10
  • Contact: 张晗。E-mail:zhanghan9157@gmail.com E-mail:zhanghan9157@gmail.com

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Abstract: Objective To investigate the significance and effect of emodin on the activation of nuclear factor-κB (NF-κB) in corneas of keratitis rats. Methods Pseudomonas aeruginosa lipopolysaccharide (LPS)-induced keratitis models were established in Wistar rats. Before LPS exposure (30 minutes), emodin and its vehicle were injected into subconjunctival tissues of rats in treatment group and inflammation group. At different time after LPS challenge, rats were examined with slit lamp microscope to observe the severity of ocular inflammation. The expression of inhibitor NF-κBα (IκBα) was determined with Western blotting, and intercellular adhesion molecule-1 (ICAM-1) was detected with immunohistochemical staining. Results The rat models of keratitis were successfully established. LPS could induce a severe corneal inflammatory response, which could be improved by emodin. IκBα level was significantly decreased after LPS exposure, and the degeneration of IκBα induced by LPS was markedly inhibited by pretreatment with emodin (P<0.01). Furthermore, the positive cells of ICAM-1 staining were significantly decreased in treatment group as compared with inflammation group. Conclusion LPS takes part in the activation of NF-κB in keratitis corneas, and emodin can partly inhibit this activation and result in therapeutic effect.

Key words: Emodin, Cytokines, Nuclear factor-κB, Keratitis

CLC Number: 

  • R772.2
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