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Inhibitory effect and its mechanism of emodin on the proliferation of human osteosarcoma cell line

XU Liangzhi1, CHEN Kunlun2   

  1. 1. Department of Orthopaedics, Nuclear Industry 417 Hospital, Lintong 710600, Shaanxi, China;
    2. Department of General Surgery, the Second Affiliated Hospital, School of Medicine,
    Xian Jiaotong University, Xi'an 710004, Shaanxi, China
  • Received:2013-12-02 Online:2014-06-10 Published:2014-06-10

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Abstract: Objective  To explore the inhibitory effect of emodin on the proliferation of human osteosarcoma cells SOSP9607 and the related mechanisms. Methods  Human osteosarcoma cells SOSP9607 were divided randomly into control group and treatment groups (emodin: 25, 50 and 100μmol/L). The growth inhibition ratio (IR) of emodin on osteosarcoma cells was detetced with MTT assay. The apoptosis was analyzed with flow cytometry. The changes of expressions of caspase-3, caspase-9, ERK1/2 and p-ERK1/2 were tested with Western blotting. Results  All of the cells in the treatment groups were obviously inhibited compared to those in control group, which was dose-dependent. Compared with control group, the apoptosis ratio of treatment group increased. Western blotting showed that the expressions of caspase-3 and caspase-9 protein increased in treatment group compared to control group, while the expression of p-ERK1/2 protein decreased. Conclusion  Emodin could inhibit the proliferation of human osteosarcoma cell by suppressing ERK signaling pathway and activating caspase-3 and caspase-9.

Key words: Emodin, ERK1/2, Apoptosis, Osteosarcoma

CLC Number: 

  • R738.1
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