JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (4): 11-16.doi: 10.6040/j.issn.1671-7554.0.2015.796

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Therapeutic effects of AG490 on experimental autoimmune myasthenia gravis in Lewis rats

CHEN Hui1, LI Heng1, YUE Longtao2, ZHANG Min1, WANG Congcong1, WANG Shan1, ZHANG Peng1, DUAN Ruisheng1   

  1. 1. Department of Neurology;
    2.Central Laboratory, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, Shandong, China
  • Received:2015-08-21 Online:2016-04-10 Published:2016-04-10

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Abstract: Objective To investigate the effect of AG490 on Lewis rats with experimental autoimmune myasthenia gravis(EAMG). Methods The EAMG model was established by immunization of Lewis rats with R97-116 peptide. Twelve rats were randomly divided into two groups(n=6 in each group), i.e, the control group and the AG490 treatment group. The levels of intracellular IL-6 and IL-21 were analyzed by flow cytometry. The lymphocyte proliferation was determined by CCK-8 and CFSE. Anti-R97-116 IgG and subtypes in the serum were detected by ELISA. Results From day 13 after immunization, the clinical symptoms of the AG490 group were obviously ameliorated, especially on day 27, 29, 31, 33, 37, 39 and 41(P<0.05), compared with the control group. Besides this, the AG490 treatment group also showed decreased secretion of IL-6 and IL-21(P<0.01), reduced anti-R97-116 peptide IgG and IgG2b(P<0.01), and suppressed lymphocyte(in particular CD4+ T cells)proliferation, compared with the control group. Conclusion AG490 can ameliorate EAMG by down-regulating the secretion of IL-6 and IL-21, which further reduces anti-R97-116 peptide IgG and IgG2b.

Key words: Anti-R97-116 antibody, AG490, IL-21, Experimental autoimmune myasthenia gravis, IL-6

CLC Number: 

  • R746.1
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