JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (4): 6-10.doi: 10.6040/j.issn.1671-7554.0.2015.1000

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Effect of X-ray on the activity of MMP-2 and Cho/Cr ratio of U87 glioma multicellular spheroid

XU Yanjie1, CUI Yi1, LI Hongxia1, SHI Wenqi1, LI Fuyan1, WANG Jianzhen2, ZENG Qingshi1   

  1. 1. Department of Radiology;
    2. Department of Radiotherapy, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-10-21 Online:2016-04-10 Published:2016-04-10

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Abstract: Objective To establish the U87 glioma multicellular spheroid(MCS)model and to explore the effect of various dosages of X-ray on the activity of MMP-2 and water-soluble metabolites of U87 glioma multicellular spheroids. Methods The U87 glioma MCS model was established and irradiated by X-ray of 0, 1, 5 and 10 Gy. The 0 Gg group served as the control group. Perchloric acid extracting(PCA)method was used to extract water-soluble metabolites, including choline(Cho), creatine(Cr), and N-acetylaspartate(NAA), which were observed using in vitro 14.7T high resolution 1H-MRS. The MMP-2 expression in U87 MCS model was detected with gelatin zymography. Results U87 glioma MCS model was established successfully. With the increase of X-ray doses, the Cho/Cr ratio rose from 2.74±0.02(1 Gy), 4.32±0.17(5 Gy)to 4.92±0.07(10 Gy); compared with the control group(2.10±0.29), the Cho/Cr ratio increased in the other groups［P=0.033(1 Gy), P=0.000(5 Gy), P=0.000(10 Gy)］. Compared with the control group, the activity of MMP-2 also increased(P=0.000). Conclusion Sublethal irradiation promotes the activity of MMP-2 of U87 MCS and elevates the Cho/Cr ratio.

Key words: Glioma, Multicellular spheroid, Proton magnetic resonance spectroscopy, Matrix metalloproteinase 2, Cho/Cr

CLC Number: 

  • R445.2
[1] Chinot OL, Wick W, Mason W, et al. Bevacizumab plus radiotherapy-temozolomide for newly diagnosed glioblastoma[J]. N Engl J Med, 2014, 370(8): 709-722.
[2] Badiga AV, Chetty C, Kesanakurti D, et al. Mmp-2 sirna inhibits radiation-enhanced invasiveness in glioma cells[J]. PloS One, 2011, 6(6): e20614. doi: 10.1371/journal.pone.0020614.
[3] Pei J, Park IH, Ryu HH, et al. Sublethal dose of irradiation enhances invasion of malignant glioma cells through p53-mmp 2 pathway in U87MG mouse brain tumor model[J]. Radiat Oncol, 2015, 10: 11. doi: 10.1186/s13014-015-0475-8.
[4] 高峰. 制备和培养多细胞肿瘤球体的简易方法[J]. 黑龙江医学, 2001, 25(7): 481-482. GAO Feng. Simple method for preparation and culture of multi -cellular tumor spheroid[J]. Hei Long Jiang Medical Journal, 2001, 25(7): 481-482.
[5] Xu ZF, Pan AZ, Yong F, et al. Human umbilical mesenchymal stem cell and its adipogenic differentiation: Profiling by nuclear magnetic resonance spectroscopy[J]. World J Stem Cells, 2012, 4(4): 21-27.
[6] Cao Z, Wu LP, Li YX, et al. Change of choline compounds in sodium selenite-induced apoptosis of rats used as quantitative analysis by in vitro 9.4t mr spectroscopy[J]. World J Gastroenterol, 2008, 14(24): 3891-3896.
[7] Jagtap JC, Parveen D, Shah RD, et al. Secretory prostate apoptosis response(par)-4 sensitizes multicellular spheroids(mcs)of glioblastoma multiforme cells to tamoxifen-induced cell death[J]. FEBS Open Bio, 2015, 5: 8-19. doi: 10.1016/j.fob.2014.11.005.
[8] Carlsson J. Proliferation gradient in three dimensional colonies of cultured human glioma cells[J]. Int J Cancer, 1977, 20(1): 129-136.
[9] Freyer JP, Sutheriand RM. Proliferative and clonogeneity of cells from EMT6/RO multicellular spheroids induced by the glucose and oxygen supply[J]. Cancer Res, 1986, 46(7): 3513-3520.
[10] Sainan C, Qiang L, Yubo L, et al. Correlation of fractional anisotropy and metabolite concentrations measured using 1h-mrs of cerebral white matter in healthy adults[J]. Biomed Mater Eng, 2014, 24: 3017-3024.
[11] Zhang Z, Zeng Q, Liu Y, et al. Assessment of the intrinsic radiosensitivity of glioma cells and monitoring of metabolite ratio changes after irradiation by 14.7-T high-resolution 1H MRS[J]. NMR Biomed, 2014, 27(5): 547-552.
[12] Doblas S, He T, Saunders D, et al. In vivo characterization of several rodent glioma models by 1H MRS[J]. NMR Biomed, 2012, 25(4): 685-694.
[13] Guan L, Li ZY, Zhang YL, et al. Early biomarkers in 1h nuclear magnetic resonance spectroscopy of striatal pathological mechanisms after acute carbon monoxide poisoning in rats[J]. Biomed Environ Sci, 2015, 28(10): 728-737.
[14] Kinoshita Y, Yokota A. Absolute concentrations of metabolites in human brain tumors using in vitro proton magnetic resonance spectroscopy[J]. NMR Biomed, 1997, 10(1): 2-12.
[15] Jarskog LF, Dong Z, Kangarlu A, et al. Effects of davunetide on N-acetylaspartate and choline in dorsolateral prefrontal cortex in patients with schizophrenia[J]. Neuropsychopharmacology, 2013, 38(7): 1245-1252.
[16] Tarnawski R, Sokol M, Pieniazek P, et al. H-1-mrs in vivo predicts the early treatment outcome of postoperative radiotherapy for malignant gliomas[J]. Int J Radiat Oncol Biol Phys, 2002, 52(5): 1271-1276.
[17] 刘伟, 王长坤, 只达石. MMP-2、MMP-9在人脑胶质瘤及其浸润组织中的表达和临床意义[J].中风与神经疾病杂志, 2009, 26(6): 689-692. LIU Wei, WANG Changkun, ZHI Dashi. The expression and clinical significance of the MMP-2 and MMP-9 in human glioma and gliomas invasive tissue[J]. J Apoplexy and Nervous Diseases, 2009, 26(6): 689-692.
[18] Wild-Bode C, Weller M, Rimner A, et al. Sublethal irradiation promotes migration and invasiveness of glioma cells: implications for radiotherapy of human glioblastoma[J]. Cancer Res, 2001, 61(6): 2744-2750.
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