JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 6-11.doi: 10.6040/j.issn.1671-7554.0.2014.826

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SN-38-loaded electrospun composite nanofiber scaffolds for the treatment of human glioma cells in vitro

ZHU Xiaodong, XIA Tongliang, YAO Qingyu, LI Haoyuan, WANG Benlin, NI Shilei, WANG Jiangang, LI Xingang, SU Wandong   

  1. Department of Neurosurgery, Qilu Hospital of Shandong University; Institute for Brain Science, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-17 Revised:2015-03-09 Online:2015-04-10 Published:2015-04-10

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Abstract: Objective To establisht an effectively controlled release system which was based on 7-ethyl-10-hydroxyl camptothecin (SN-38)-loaded electrospun composite nanofiber with poly (ε-caprolactone) (PCL)/poly (D, L-lactide) (PDLLA) and to evaluate its antitumor activity against U-251 glioma cells in vitro. Methods SN-38-loaded PCL/PDLLA fibers were manufactured by electrospinning. The physical status of SN-38 within the polymeric fibers was investigated by different scanning calorimetry (DSC) and fourier transform infrared (FT-IR). The static wettability of the membrane was measured by contact angle analyzer. The release speeds of two samples were determined, and their cytotoxicity against U-251 glioma cells was assessed in vitro. Results Uniform and smooth SN-38-loaded PCL/ PDLLA fibers were obtained. Cell toxicity test results showed that the SN-38-loaded PCL/PDLLA fibers could continuously inhibit the U-251 glioma cells. Due to different drug-loading rates, different membranes showed various release time. The antitumor activity of the 2 wt.% SN-38-loaded PCL/PDLLA nanofiber meshes were controlled and sustained. Conclusions Using SN-38-loaded composite nanofiber of PCL/PDLLA as a sustained delivery system is feasible. While the drug-loading rate is augmented, the release time of drug within the fibers will be increased accordingly.

Key words: Poly (ε-caprolactone), Electrospinning, Glioma, Poly (D,L-lactide), 7-ethyl-10-hydroxyl camptothecin

CLC Number: 

  • R739.41
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