JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 12-15.doi: 10.6040/j.issn.1671-7554.0.2015.036

Previous Articles     Next Articles

Experimental research on biological activities of glioma cells inhibited by TRAIL in combination with paclitaxel

XING Deguang1, MA Ermeng2, WANG Moulong3, SUN Zhongzheng1, FAN Mingde1, ZANG Yizheng1, WANG Chengwei1   

  1. 1. Department of Neurosurgery, The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Neurosurgery, The First Hospital of China Medical University, Shenyang 110001, Liaoning, China;
    3. Department of Neurosurgery, Central Hospital of Shandong Energy Zibo Mining Group Co., Ltd, Zibo 255120, Shandong, China
  • Received:2015-01-12 Revised:2015-04-02 Online:2015-04-10 Published:2015-04-10

PDF (PC)

221

Abstract: Objective To explore the effects of paclitaxel (PX) in combination with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on glioma U251 cell apoptosis and its possible mechanism. Methods The proliferation and apoptosis of U251 cells treated by TRAIL/PX in combination or alone at different concentrations were detected with modified MTT and flow cytometry. Proteins of U251 cell TRAIL death receptor DR4 and DR5 were measured with Western blot assay. Results Both TRAIL and PX had inhibitory effect on U251 cell proliferation in a concentration-dependent manner, combined TRAIL/PX treatment had remarkably higher inhibitory effect on cell growth and apoptosis of U251 cells than either drug used lone (P <0.05). Furthermore, combined TRAIL/PX treatment showed a synergistic effect on U251 to upregulate the expression of DR4 (P <0.05), but had no effect on the expression of DR5. Conclusions PX can improve the sensitivity of glioma U251 cells to TRAIL by upregulation of DR4, and then inhibit glioma cell proliferation and induce apoptosis.

Key words: Glioma, U251 cells, Hematodialysis, Parathyroid hormone, Paclitaxel, TNF-related apoptosis-inducing ligand, Apoptosis, Renal osteopathy

CLC Number: 

  • R739.41
[1] Talibi SS, Aweid B, Aweid O. Prospective therapies for high-grade glial tumours: A literature review[J]. Ann Med Surg(Lond), 2014, 3(3): 55-59.
[2] Khan M, Bi Y, Qazi JI, et al. Evodiamine sensitizes U87 glioblastoma cells to TRAIL via the death receptor pathway[J]. Mol Med Rep, 2015, 11(1): 257-262.
[3] Ismail B, Fagnere C, Limami Y, et al. 2'-Hydroxy-4-methylsulfonylchalcone enhances TRAIL-induced apoptosis in prostate cancer cells[J]. Anticancer Drugs, 2015, 26(1): 74-84.
[4] Xu F, Wang F, Yang T, et al. Differential drug resistance acquisition to doxorubicin and paclitaxel in breast cancer cells[J]. Cancer Cell Int, 2014, 14(1): 538.
[5] Hunter TB, Manimala NJ, Luddy KA, et al. Paclitaxel and TRAIL synergize to kill paclitaxel-resistant small cell lung cancer cells through a caspase-independent mechanism mediated through AIF[J]. Anticancer Res, 2011, 31(10): 3193-3204.
[6] Qiu B, Sun X, Zhang D, et al. TRAIL and Paclitaxel synergize to kill U87 cells and U87-derived stem-like cells in vitro[J]. Int J Mol Sci, 2012, 13(7): 9142-9156.
[7] Wang F, Lin J, Xu R. The molecular mechanisms of TRAIL resistance in cancer cells: help in designing new drugs[J]. Curr Pharm Des, 2014, 20(42): 6714-6722.
[8] Gretney E, Takeda K, Smyth MJ. Cancer: novel therapeutic strategies that exploit the TNF-related apoptosis-inducing ligand (TRAIL)/TRAIL receptor pathway[J]. Int J Biochem Cell Biol, 2007, 39(2): 280-286.
[9] van Roosmalen IA, Quax WJ, Kruyt FA. Two death-inducing human TRAIL receptors to target in cancer: similar or distinct regulation and function?[J]. Biochem Pharmacol, 2014, 91(4): 447-456.
[10] Holland PM. Death receptor agonist therapies for cancer, which is the right TRAIL?[J]. Cytokine Growth Factor Rev, 2014, 25(2): 185-193.
[11] Zhan C, Wei X, Qian J, et al. Co-delivery of TRAIL gene enhances the anti-glioblastoma effect of paclitaxel in vitro and in vivo[J]. J Control Release, 2012, 160(3): 630-636.
[12] Yoshida T, Zhang Y, Rivera RL, et al. Repeated treatment with subtoxic doses of TRAIL induces resistance to apoptosis through its death receptors in MDA-MB-231 breast cancer cells[J]. Mol Cancer Res, 2009, 7(11): 1835-1844.
[13] Tu LX, Wu YO, Zhang LJ. Experimental study on apoptosis of SGC-7901 cell induced by TRAIL combined with paclitaxel[J]. Chinese Journal of Immunology, 2012, 28(4): 308-311.
[14] Huang T, Li XH, Li XC, et al. Study on the apoptosis of osteosarcoma cell induced by combination of TRAIL and Paclitaxel[J]. China Journal of Modern Medicine, 2013, 23(2): 42-45.
[15] Tan QP, Liu Y, Qiu YZ, et al. Synergistic interactions of TRAIL and paclitaxel on the nasopharyngeal carcinoma cell lines in vitro[J]. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi, 2012, 26(7): 319-322.
[16] Sun X, Pang Z, Ye H, et al. Co-delivery of pEGFP-hTRAIL and paclitaxel to brain glioma mediated by an angiopep-conjugated liposome[J]. Biomaterials, 2012, 33(3): 916-924.
[1] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[2] WANG Lin-Lin, SUN Mei-Li, SUN Yu-Ping, ZHANG Nan, LIU Chuan-Yong. Expressions of α-Tubulin and γ-Tubulin in human Glioma and their relation with Survivin [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 103-.
[3] Gang LI,Hao XUE,Wei QIU,Rongrong ZHAO. Research advances in the formation of glioma immunosuppressive microenvironment [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 67-73.
[4] Qiang WU,Zekun HE,Ju LIU,Xiaomeng CUI,Shuang SUN,Wei SHI. A research on multi-modal MRI analysis based on machine learning for brain glioma [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 81-87.
[5] Anjing CHEN,Xun ZHANG. The new strategies of targeting SUMOylation in the treatment of glioma [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 88-94.
[6] Tao JIANG. The application of brain-like intelligence in the frontiers of brain science [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 10-13.
[7] XU Jixi, CHEN Weijian. Diffuse midline glioma with H3 K27M mutation in the spinal cord: a case report [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 96-101.
[8] ZHANG Liang, XU Min, ZHUANG Xianghua, LOU Fuchen, LOU Nengjun, LÜ Li, GUO Wenjuan, ZHENG Fengjie, CHEN Shihong. An investigation of endoplasmic reticulum stress and apoptosis in diabetic peripheral neuropathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 13-17.
[9] LI Rui, MA Weihong, REN Manyi, ZHAO Mengmeng, JIANG Shan, JU Yuanyuan, GUO Ying, SUN Zhaohui, SUI Shujian. Correlation between TWEAK and cardiac remodeling in essential hypertensive patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 49-55.
[10] GUO Hehe, SUN Zhiqiang, LIU Yanjuan, LIU Yichen, LI Guang, ZHENG Fang. Norcantharidin influences the expression of Notch signal pathway in multiple myeloma U226 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 32-37.
[11] TU Yunhua, CHEN Liyuan, WANG Xi, ZHOU Ying, KANG Yingqian, XUE Yuecui, RONG Dongyun, YE Zhenyuan, CAO Yu. Effect of ar-turmerone derivatives on proliferation and apoptosis in human melanoma WM35 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(2): 68-73.
[12] WANG Chaochao, TIAN Hailong, JIANG Huifeng, GUO Wenqiang, WANG Zhigang. Clinical significance of sodium fluorescein-guided resection of high-grade glioma under “yellow fluorescence” mode [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 32-37.
[13] GONG Luwei, ZHOU Lizhen, SU Guohai. Perindopril ameliorates cardiac dysfunction via Akt-FoxO1 pathway in diabetic cardiomyopathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 65-70.
[14] ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia. Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 49-53.
[15] ZHANG Kun, ZHAGN Xiaoguo, CUI Xingxing, CHEN Shijun. Expression of apoptosis-related genes in peripheral blood mononuclear cells of patients with HBV infection and the relationship with chronicity of hepatitis B [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 38-42.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd