JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (5): 8-12.doi: 10.6040/j.issn.1671-7554.0.2016.1546

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Effects of GCH1 on atrial autonomic nerve remodeling by regulating tetrahydrobiopterin in A-TP canines

WEI Jinqiu, ZHANG Yujiao, LI Zhan, WANG Ximin, CHEN Linlin, DU Juanjuan, ZHANG Yong, XIE Xinxing, HOU Yinglong   

  1. Department of Cardiology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2016-11-22 Online:2017-05-10 Published:2017-05-10

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Abstract: Objective To investigate the expression of guanosine triphosphate cyclohydrolase I(GCH1)on atrial autonomic nerve remodeling(ANR)in atrial-tachypacing(A-TP)canines. Methods A total of 24 mongrel dogs were randomly divided into 4 groups: atrial tachypacing group(A-TP), control group, GCH1 over-expression group and lenti-anti-GCH1 group. Each group had 6 dogs. Canines in the A-TP group had continuous right A-TP(400 beats/min)for 4 weeks, and then atrial tissue samples were harvested from right superior fat pad(RSFP). Canines in the GCH1 over-expression group were injected with lenti-GCH1 into RSFPs. Canines in the lenti-anti-GCH1 group were injected with lenti-anti-GCH1 into RSFPs. After 2 weeks, canines were anesthetized and atrial samples were collected. The mRNA expression was detected with qRT-PCR, the protein expression was detected with Western blotting, nerve density was evaluated with immunohistochemical staining, and Tetrahydrobiopterin(BH4)content was determined with Elisa analysis. Results GCH1 expression in the A-TP group was obviously lower than that in the control group(P<0.05). Compared with the control group, GCH1 over-expression group had longer atrial effective refractory period, decreased PGP9.5 expression, but increased BH4 content. Compared with the control group, the lenti-anti-GCH1 group had increased PGP9.5 expression and decreased BH4 content. Conclusion Rapid pacing of right atrium can lead to downregulation of GCHI. GCH1 may inhibit ANR during atrial fibrillation by regulating BH4 content.

Key words: Atrial fibrillation, Cardiac autonomic nerve remodeling, GTP cyclohydrolase 1, Tetrahydrobiopterin

CLC Number: 

  • R541.7
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