JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 41-45.doi: 10.6040/j.issn.1671-7554.0.2015.152

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Role of Sirt3 on the senescence of human umbilical vein endothelial cells

LIU Hui, CHEN Tongshuai, LI Na, WANG Shujian, LI Jingyuan, BU Peili   

  1. Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-02-05 Revised:2015-03-19 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To investigate the role of Sirtuin3(Sirt3) on the senescence of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro. Sirt3-siRNA was synthesized by reagent company. Sirt3-siRNA with the best inhibition efficiency was screened out with Western blotting assay. HUVECs were divided into negative control group and Sirt3-siRNA group. Western blotting assay was used to evaluate the protein expressions of Sirt3, P16 and P21. Senescence β-gal staining was used to identify the aging status of HUVECs. The intracellular ROS was detected with 2',7'-dichlorodi-hydro-fluorescein diacetate (DCFH-DA). Results Compared with the negative control group, the Sirt3-siRNA group showed decreased protein expression of Sirt3 (P<0.05), increased positive rate of β-gal staining, elevated P16 and P21 expressions (P<0.05), and higher level of intracellular ROS. Conclusion Sirt3 can delay the senescence of HUVECs by regulating ROS generation.

Key words: P21 protein, Sirtuin3, Cell senescence, Human umbilical vein endothelial cells, P16 protein, Reactive oxygen species

CLC Number: 

  • R543.1+2
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