JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 21-26.doi: 10.6040/j.issn.1671-7554.0.2014.581

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Effects of TMPyP4-PDT on human immortal cervical epithelial H8 cells

ZONG Liju1, ZHANG Youzhong1, WANG Jie1, WU Shuxia1, LIU Hongli1, ZHANG Lu2   

  1. 1. Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu, China
  • Received:2014-09-05 Revised:2014-10-29 Published:2015-01-10

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Abstract: Objective To investigate the effects of photosensitizer 5, 10, 15, 20-Tetrakis (1-methylpyridinium-4-yl) porphyrin based-photodynamic therapy (TMPyP4-PDT) on the proliferation and apoptosis of human immortal cervical epithelial H8 cells. Methods The morphological changes were observed by inverted microscope. Proliferative viability was evaluated with Cell Counting Kit-8. Apoptosis was assessed by Annexin V-FITC/PI Apoptosis Detection Kit. The p16INK4a expression was evaluated by immunocytochemistry Strept Avidin-Biotin-enzyme Complex (SABC) method. Human telomerase reverse transcriptase (hTERT) and p21 expression were analyzed by Western blotting. Results TMPyP4-PDT could inhibit H8 cellular proliferative viability and induce apoptosis in a TMPyP4 dose-dependent manner. Immunocytochemistry analysis revealed that TMPyP4-PDT could reduce the positive rate of p16INK4a protein in H8 cells. Western blotting results indicated that TMPyP4-PDT could downregulate hTERT protein expression while upregulate p21 protein in H8 cells. Conclusion TMPyP4-PDT can inhibit H8 cell proliferation and induce apoptosis. The possible mechanism is related to down-regulation of p16INK4a and hTERT, as well as up-regulation of p21 protein.

Key words: 5, 10, 15, 20-Tetrakis (1-methylpyridinium-4-yl) porphyrin- photodynamic therapy, p21 protein, Cervix neoplasms, H8 cells, Human telomerase reverse transcriptase, p16INK4a protein

CLC Number: 

  • R454.2
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