JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 61-64.doi: 10.6040/j.issn.1671-7554.0.2014.641

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Beta-synuclein promotes the expression of vesicular monoamine transporter-2

YANG Lu1, ZHOU Yingze2, NI Ming1, FAN Xiushuang1, MAN Jianmei1, GUO Juntang1   

  1. 1. Department of Pathophysiology, School of Basic Medicine, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Pathology, Anyue County People's Hospital, Anyue 642350, Sichuan, China
  • Received:2014-09-24 Revised:2014-12-24 Online:2015-04-10 Published:2015-04-10

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Abstract: Objective To investigate the effect of β-synuclein on the expression of vesicular monoamine transporter-2(VMAT2). Methods The effects of β-synuclein stable expression on the intracellular ROS level were detected by dichlorofluorescein diacetata(DCFH-DA). The expression of VMAT2 was detected by double immunofluorescence, immunohistochemistry and Western blotting in SH-SY5Y cells which were transfected stably with β-synuclein and normal SH-SY5Y cells. Results The intracellular ROS level in the cells stably expressing β-synuclein was significantly lower than that of normal SH-SY5Y cells.The expression of β-synuclein in the SH-SY5Y cells which were stably transfected with β-synuclein was higher than that of normal SH-SY5Y cells. Conclusion β-synuclein can reduce the content of DA in cytosolic by promoting the expression of VMAT2, which can inhibit the generation of ROS. It plays an important protective role in the process of dopamine neurons' degeneration in Parkinson's disease.

Key words: Beta-synuclein, Reactive oxygen species, Parkinson's, disease, Vesicular monoamine transporter-2

CLC Number: 

  • R741
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