JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (8): 18-23.doi: 10.6040/j.issn.1671-7554.0.2016.1225

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Protection of thioctic acid in mitochondria in the rat model of Parkinsons disease

GOU Yun1, ZHOU Bo2, WEI Cao2, CHEN Yunhua1, XU Li3, LIU Fen4, ZHANG Chunlin2, WEN Min1   

  1. 1. Department of Anatomy;
    2. Department of Biology;
    3. Department of Neurology, Affiliated Hospital;
    4.Department of Pediatrics, Affiliated Hospital, Guizhou Medical University, Guiyang 550004, Guizhou, China
  • Received:2016-09-29 Online:2017-08-10 Published:2017-08-10

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Abstract: Objective To investigate the change of mitochondria in midbrain of rat model with Parkinsons disease(PD)and the protective effect of lipoic acid on mitochondria. Methods Eighty male SD rats were randomly divided into sham group(20 rats)and model group(60 rats).In model group, 6-Hydroxydopamine(6-OHDA)was injected into the right striatum to establish the unilateral mesencephalic lesions model.The rats in sham group were injected with the same dose of saline by the same method. Four weeks after 6-OHDA lesion, 40 of the successful model rats(screened by Apomorphine-induced rotation test)were selected and divided into PD group(20 rats)and treatment group(20 rats). The rats in treatment group were intraperitoneally injected with lipoic acid(50 mg/kg)daily for 14 days. The rats in PD group were injected with the same dose saline at the same time. The motor coordination was observed by cylinder test. The ATP level and the mitochondrial membrane potential in right midbrain were detected by chemiluminescence and probe JC-1 methods respectively. The expression of TH in midbrain was detected by Western blotting and immunohistochemical staining. The expression of HSP60 in midbrain was detected by Western blotting. Results Compared with sham group, the number of touching the cylinder wall with left front leg, ATP level, the mitochondrial membrane potential and the expression of TH in PD group decreased at the same time(P<0.05), but the expression of HSP60 did not change significantly(P>0.05). Impairment of behavior,ATP level,mitochondrial membrane potential and the expression of TH recovered simultaneously after the treatment of thioctic acid(P<0.05), while 山 东 大 学 学 报 (医 学 版)55卷8期 -勾云,等.硫辛酸对帕金森病大鼠黑质线粒体的保护作用 \=-the expression of HSP60 decreased too slightly(P>0.05). Conclusion The function of mitochondria in substantia nigra of PD rats decreases, but the number of mitochondria does not change obviously. Thioctic acid has beneficial therapeutic effect on PD by ameliorating mitochondrial function.

Key words: 6-Hydroxydopamine, Parkinsons disease, Thioctic acid, Mitochondria

CLC Number: 

  • R749.1
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