JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (8): 24-29.doi: 10.6040/j.issn.1671-7554.0.2017.059

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IL-17F promotes the proliferation, mineralization and expressions of Runx2 and Osterix in rat osteoblasts

JI Yuanyuan1, BAO Jingbo2, ZHAO Xu1, CAO Luning1, CUI Min1, ZHANG Na1, YU Lingzhi1   

  1. 1. Department of Pain Management, Jinan Central Hospital Affliliatedto Shandong University, Jinan 250013, Shandong, China;
    2. Hospital of Yantai Vocational College, Yantai 264025, Shandong, China
  • Received:2017-01-16 Online:2017-08-10 Published:2017-08-10

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Abstract: Objective To investigate the effects of interleukin-17F(IL-17F)on the proliferation, mineralization and expressions of Runx2 and Osterix in rat osteoblasts. Methods Primarycalvarial osteoblasts were isolated from neonatal Wistarrats<24 h, cultured with low glucose DMEM medium containing 10% fetal bovine serum, and the medium was replaced every 3 days. The cells were passaged when the cell fusion area reached about 80%. The fourth generation osteoblasts were divided into 6 groups according to the concentration of IL-17F in the culture medium: 0 ng/mL group(control group), 1 ng/mL group, 10 ng/mL group, 20 ng/mL group, 50 ng/mL group, and 100 ng/mL group. After 3 days, the proliferation rate of osteoblasts was detected with CCK-8 kit. The mRNA transcription and protein expressions of Runx2 and Osterix were detected with real time fluorescent quantitative PCR and Western blotting, respectively. After 10days, the mineralized nodules were stained with Alizarin red in the control group and 100 ng/mL group. Results The cell proliferation rate was higher in 20 ng/mL, 50 ng/mL and 100 ng/mL groups than in the control group(P<0.05), in a concentration-dependent manner. The levels of mRNA transcription and protein expressions of Runx2 and Osterix in 50 ng/mL group and 100 ng/mL group were higher than those in the control group(P<0.05). The positive 山 东 大 学 学 报 (医 学 版)55卷8期 -姬原原,等.白介素17F促进大鼠成骨细胞增殖、矿化和Runx2、Osterix的表达 \=-staining area of mineralized nodules in 100 ng/mL group was larger than that in the control group. Conclusion Interleukin-17F can promote the osteogenesis of rat osteoblasts in vitro.

Key words: Runx2, Osterix, Mineralization, Interleukin-17F, Osteoblast

CLC Number: 

  • R589
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