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Table of Content

      
    10 August 2017
    Volume 55 Issue 8
    CYLD improves bladder cancer gemcitabine chemosensitivity via suppressing autophagy
    YIN Lei, YIN Rui, LI Wenjia, LIU Shuai, L(¨overU)Jiaju
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  1-6.  doi:10.6040/j.issn.1671-7554.0.2016.378
    Abstract ( 979 )   PDF (4718KB) ( 184 )   Save
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    Objective To explore the tumor suppressor CYLD in improving bladder cancer gemcitabine chemosensitivity. Methods The CYLD over-expressing plasmid was constructed and transfected into human bladder cancer cell lines T24 and 253J, then the cells were divided into control group treated with liposome, empty plasmid transfection group and CYLD group. The effect of CYLD on the migration/invasion ability of bladder cancer cells was measured by Transwell assays. The chemosensitivity to gemcitabine was tested by CCK-8 assay. Autophagy-related proteins were detected by Western blotting, acridine orange staining, and transmission electron microscopy. Results Compared with the control group and empty plasmid transfection group, CYLD group had significantly decreased cell numbers in Transwell chambers(P<0.05); significantly reduced 50% cell growth inhibition in 24 h(IC50)(P<0.05); decreased expressions of LC3 and Beclin 1, but elevated expression of p62; decreased autophagy lysosomes and autophagic vacuoles. Conclusion CYLD can decrease the ability of migration/invasion and improve gemcitabine chemosensitivity by sup- 山 东 大 学 学 报 (医 学 版)55卷8期 -殷雷,等.CYLD抑制自噬提高膀胱癌细胞吉西他滨化疗敏感性 \=-pressing autophagy in bladder cancer cells.
    Rescue of 3D recombinant enterovirus 71
    BAI Yongjuan, ZHUANG Zhichao, HAO Shubin, WANG Lihong, LI Chun, LU Yan, ZHAO Li, WANG Zhiyu, WEN Hongling
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  7-12.  doi:10.6040/j.issn.1671-7554.0.2016.1383
    Abstract ( 831 )   PDF (3654KB) ( 178 )   Save
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    Objective To replace 3D region of enterovirus 71(EV71)high virulent strain SDLY107 with low virulent strain SDLY1 by reverse genetics technology, and to rescue recombinant virus, providing a platform for investigation on EV71 3D protein. Methods The recombine fragment of 3D protein was obtained by overlap PCR, and then inserted into pMD19-T vector. 3D region of pMD19-T-107 containing the full-length gene of SDLY107 was replaced with recombine fragment through double digestion and ligase T4 to construct pMD19-T-107(1-3D). The infectious RNA was obtained by in vitro transcription, then transfected into Vero cells and gained recombinant virus particles after three passages. PCR and qRT-PCR were performed for recombinant virus identification. Results ApaI-3D anterior region(600 bp), 3D region(1 400 bp)and 3'UTR-XbaI region(200 bp)were obtained by PCR, and 1-3D(2 100 bp)was obtained by overlap PCR. The recombinant plasmid was identified by double enzyme digestion(7 400 and 2 700 bp), and sequencing results further confirmed the successful replacement of 3D region. Cell shrinkage, roundness and refractive index enhancement were observed after transfecting the infectious clone into Vero cells and cultivating to the third generation. Identification of recombinant virus by PCR gained fragment of 226 bp; qRT-PCR detected the amount of 山 东 大 学 学 报 (医 学 版)55卷8期 -白永娟,等.肠道病毒71型3D重组病毒的拯救 \=-virus increased slowly from 24 h to 48 h and increased rapidly from 48 h to 72 h, and the amount of virus no longer growed after 72 h. Conclusion The recombinant virus SDLY107(1-3D)is rescued successfully. The study provids the basis for further research on the virulence of 3D protein and its role in the pathogenesis of EV71.
    An investigation of endoplasmic reticulum stress and apoptosis in diabetic peripheral neuropathy
    ZHANG Liang, XU Min, ZHUANG Xianghua, LOU Fuchen, LOU Nengjun, LÜ Li, GUO Wenjuan, ZHENG Fengjie, CHEN Shihong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  13-17.  doi:10.6040/j.issn.1671-7554.0.2017.490
    Abstract ( 624 )   PDF (1915KB) ( 181 )   Save
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    Objective To observe endoplasmic reticulum stress(ERS)and apoptosis-related protein in diabetic peripheral neuropathy(DPN)in animal models and to investigate the possible mechanism. Methods A total of 36 Wistar rats were divided into 3 groups: control group(n=12, CON group), diabetic mellitus group (n=12, DM group), and diabetic peripheral neuropathy group(n=12, DPN group). The blood glucose(BG)and motor nerve conduction velocity(MNCV)were measured. The proteins, including CHOP, PERK, Bax, Bcl-2 and Caspase-12 were determined with Western blotting. Results Compared with CON group, the DM group had greatly increased BG and significantly decreased MNCV(both P<0.001); higher expression of CHOP, PERK, Bax, and Caspase-12(P=0.003, P<0.001, P<0.001, P<0.001), but lower expression of Bcl-2(P<0.001). Compared with the DM group, the DPN group had elevated expression of CHOP, PERK, Bax, and Caspase-12(P<0.001, P=0.001, P<0.001, P=0.002), but lower expression of Bcl-2(P<0.001). Conclusion In animal models of DPN, the ERS in sciatic nerve is increases, and the level of apoptosis also increases, indicating that ERS is involved in the occurrence of DPN.
    Protection of thioctic acid in mitochondria in the rat model of Parkinsons disease
    GOU Yun, ZHOU Bo, WEI Cao, CHEN Yunhua, XU Li, LIU Fen, ZHANG Chunlin, WEN Min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  18-23.  doi:10.6040/j.issn.1671-7554.0.2016.1225
    Abstract ( 892 )   PDF (1737KB) ( 125 )   Save
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    Objective To investigate the change of mitochondria in midbrain of rat model with Parkinsons disease(PD)and the protective effect of lipoic acid on mitochondria. Methods Eighty male SD rats were randomly divided into sham group(20 rats)and model group(60 rats).In model group, 6-Hydroxydopamine(6-OHDA)was injected into the right striatum to establish the unilateral mesencephalic lesions model.The rats in sham group were injected with the same dose of saline by the same method. Four weeks after 6-OHDA lesion, 40 of the successful model rats(screened by Apomorphine-induced rotation test)were selected and divided into PD group(20 rats)and treatment group(20 rats). The rats in treatment group were intraperitoneally injected with lipoic acid(50 mg/kg)daily for 14 days. The rats in PD group were injected with the same dose saline at the same time. The motor coordination was observed by cylinder test. The ATP level and the mitochondrial membrane potential in right midbrain were detected by chemiluminescence and probe JC-1 methods respectively. The expression of TH in midbrain was detected by Western blotting and immunohistochemical staining. The expression of HSP60 in midbrain was detected by Western blotting. Results Compared with sham group, the number of touching the cylinder wall with left front leg, ATP level, the mitochondrial membrane potential and the expression of TH in PD group decreased at the same time(P<0.05), but the expression of HSP60 did not change significantly(P>0.05). Impairment of behavior,ATP level,mitochondrial membrane potential and the expression of TH recovered simultaneously after the treatment of thioctic acid(P<0.05), while 山 东 大 学 学 报 (医 学 版)55卷8期 -勾云,等.硫辛酸对帕金森病大鼠黑质线粒体的保护作用 \=-the expression of HSP60 decreased too slightly(P>0.05). Conclusion The function of mitochondria in substantia nigra of PD rats decreases, but the number of mitochondria does not change obviously. Thioctic acid has beneficial therapeutic effect on PD by ameliorating mitochondrial function.
    IL-17F promotes the proliferation, mineralization and expressions of Runx2 and Osterix in rat osteoblasts
    JI Yuanyuan, BAO Jingbo, ZHAO Xu, CAO Luning, CUI Min, ZHANG Na, YU Lingzhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  24-29.  doi:10.6040/j.issn.1671-7554.0.2017.059
    Abstract ( 838 )   PDF (3453KB) ( 181 )   Save
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    Objective To investigate the effects of interleukin-17F(IL-17F)on the proliferation, mineralization and expressions of Runx2 and Osterix in rat osteoblasts. Methods Primarycalvarial osteoblasts were isolated from neonatal Wistarrats<24 h, cultured with low glucose DMEM medium containing 10% fetal bovine serum, and the medium was replaced every 3 days. The cells were passaged when the cell fusion area reached about 80%. The fourth generation osteoblasts were divided into 6 groups according to the concentration of IL-17F in the culture medium: 0 ng/mL group(control group), 1 ng/mL group, 10 ng/mL group, 20 ng/mL group, 50 ng/mL group, and 100 ng/mL group. After 3 days, the proliferation rate of osteoblasts was detected with CCK-8 kit. The mRNA transcription and protein expressions of Runx2 and Osterix were detected with real time fluorescent quantitative PCR and Western blotting, respectively. After 10days, the mineralized nodules were stained with Alizarin red in the control group and 100 ng/mL group. Results The cell proliferation rate was higher in 20 ng/mL, 50 ng/mL and 100 ng/mL groups than in the control group(P<0.05), in a concentration-dependent manner. The levels of mRNA transcription and protein expressions of Runx2 and Osterix in 50 ng/mL group and 100 ng/mL group were higher than those in the control group(P<0.05). The positive 山 东 大 学 学 报 (医 学 版)55卷8期 -姬原原,等.白介素17F促进大鼠成骨细胞增殖、矿化和Runx2、Osterix的表达 \=-staining area of mineralized nodules in 100 ng/mL group was larger than that in the control group. Conclusion Interleukin-17F can promote the osteogenesis of rat osteoblasts in vitro.
    Correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α after radiotherapy
    HU Yujing, WU Dayong, ZHANG Wenyan, BIAN Yanzhu, WEI Qiang, TIAN Congna, CHANG Shengli
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  30-34.  doi:10.6040/j.issn.1671-7554.0.2016.413
    Abstract ( 881 )   PDF (5900KB) ( 378 )   Save
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    Objective To explore the correlation between 99Tcm-MNLS hypoxic scintigraphy and hypoxia-inducible factor-1α(HIF-1α)expression in the hypoxic changes of tumors after radiotherapy. Methods The tumor-bearing mice were divided into radiotherapy groups(R12h, R24h, R36h)and control groups(C12h, C24h, C36h)according to the time of developing. The radiotherapy groups received an exposure dose of 15 Gy each time, while the control groups received no radiotherapy. After 99Tcm-MNLS hypoxic scintigraphy, the radioactivity ratio of tumor to non-tumor(T/NT)was calculated. The level of HIF-1α in tumors was evaluated. After that, the correlation between T/NT and HIF-1α level was analyzed. Results The T/NT and HIF-1α level in R12h group and R36h group were higher than those in the C12h group and C36h group(all P=0.000). There was no difference between the R24h and C24h groups(P=0.612, 0.731). The T/NT was positively correlated with HIF-1α level in the radiotherapy groups(r=0.793, P=0.003), as well as in the control groups(r=0.756, P=0.004). Conclusion Uptake of 99Tcm-MNLS and expression of HIF-1α are strongly 山 东 大 学 学 报 (医 学 版)55卷8期 -胡玉敬,等.放疗后肿瘤99Tcm-MNLS乏氧显像变化与乏氧诱导因子-1α表达的相关性 \=-correlated to the changes of hypoxia in tumors after radiotherapy, and 99Tcm-MNLS hypoxic scintigraphy can provide the basis for the best time of reirradiation.
    Isolation and identification of phenolic constituents from Viscum coloratum and the antiproliferative effects on A549 cells
    HUANG Zhuqing, WU Xuewei, REN Dongmei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  35-41.  doi:10.6040/j.issn.1671-7554.0.2017.070
    Abstract ( 712 )   PDF (3697KB) ( 230 )   Save
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    Objective To isolate and purify the chemical constituents of the active fraction from Viscum coloratum, and to evaluate the apoptotic effects on A549 cells. Methods Various chromatographic methods including silica gel, sephadex LH-20 column chromatography were used for the purification of chemical constituents from the extracts of Viscum coloratum. Spectroscopic methods including NMR and MS were used for the structural elucidation of the compounds. The antiproliferative effects were evaluated by MTT assay. Flow cytometry was used for the determination of cell cycle, apoptosis and intracellular reactive oxygen species(ROS)level. Western blotting analysis was carried out for the detection of protein expression. Results Ten phenolic compounds were obtained from Viscum coloratum. Compound 1 was identified as 1,7-di-(4-hydroxyphenyl)-4E,6E-heptadiene-3-ketone. This compound exerted cytotoxicity on A549 cells dose-dependently(F=196.768, P=0.001). It increased the cell number in G2/M phase(P<0.05)and decreased the cell number in S phase(P<0.05). The intracellular ROS level induced by compound 1 increased significantly(P<0.05). The compound also induced apoptosis of A549 cells. The expressions of Caspase-9, Caspase-3 and PARP were changed under the treatment(F: 19.340, 4.388, 194.571; P: 0.001, 0.042, 0.001). Conclusion Diphenylheptanes and flavonoids are the main phenolic components of Viscum coloratum, and diphenylheptanes are the main active ingredients of Viscum coloratum against lung cancer cell proliferation.
    Preparation and characterization of PEG coating levofloxacin-liposomes
    ZHANG Xinke, CHEN Ying, LI Peng, CHEN Hongyuan, HUANG Guihua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  42-47.  doi:10.6040/j.issn.1671-7554.0.2017.339
    Abstract ( 945 )   PDF (1402KB) ( 300 )   Save
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    Objective PEG2000 was used to coat levofloxacin-liposomes(Lvf-lips)in order to improve the stability. The physico-chemical property, preliminary stability and pharmacokinetics of PEG 2000 coating levofloxacin-liposome(PEG Lvf-lips)were systematically investigated. Methods Lvf-lips were prepared by ammonium sulfate gradients method, and the optimal preparation technique of PEG Lvf-lips was screened based on the entrapment efficiency and preliminary stability. The properties of PEG Lvf-lips, such as morphology, diameter and size distribution, were observed with transmission electric microscope, laser dispersive analyzing and micro-electrophoresis apparatus. In vitro drug release of PEG Lvf-lips was performed with the dialysis bag diffusion technique. The pharmacokinetics property was also studied. Results PEG Lvf-lips had spherical shapes and uniform particle size. The entrapment efficiency was(77.97±1.09)%. The stability and sustained release effects in vitro and in vivo PEG Lvf-lips were superior to non-PEG coating liposomes. Conclusion PEG 2000 coating can significantly improve the stability of Lvf-lips, which provides a new pathway for liposome stability.
    Diagnosis and surgical treatment of pulmonary artery sling complicated with tracheal stenosis
    FENG Zhiyu, YANG Yanliang, GUO Rui, XU Huihui, ZHANG Jian, GUO Kai, WANG Guangzhen, WANG Hui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  48-51.  doi:10.6040/j.issn.1671-7554.0.2016.969
    Abstract ( 1179 )   PDF (946KB) ( 162 )   Save
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    Objective To explore the diagnosis and surgical treatment of pulmonary artery(PA)sling complicated with tracheal stenosis. Methods Clinical data of 23 cases of PA sling complicated with tracheal stenosis treated in our hospital during Jan. 2012 and Jan. 2016 were retrospectively analyzed. The patients were divided into mild stenosis group(n=13, MS group)and severe stenosis group(n=13, SS group)according to the extent of tracheal stenosis. The clinical features, surgical techniques and the treatment outcomes were reviewed. Results Altogether 9 patients(69%)in MS group had no obvious respiratory symptoms while all patients in SS group had obvious symptoms. Patients in MS group received left pulmonary artery(LPA)re-implantation alone. In SS group, patients with complete tracheal cartilage ring who had with stridor at rest underwent LPA re-implantation together with resection of narrowed segment and direct reanastomosis, 1 patient with tracheomalacia had tracheal stent placed, and the other patients received LPA re-implantation alone. All patients in MS group recovered smoothly. In SS group, 1 patient with complete tracheal ring and tracheomalacia who received LPA re-implantation without tracheal intervention failed to extubate after surgery and finally died of multiple organ failure; 3 patients who received LPA re-implantation and tracheoplasty simultaneously had an uneventful postoperative course, the patient with tracheal stent had excessive secretions in respiratory tract, received bronchoscopy repeatedly to clear sputum, and died 1 month later because of fatal bleeding from a tracheal stent suddenly; 2 patients required non-invasive continuous positive airway pressure(CPAP)to relieve respiratory distress after 山 东 大 学 学 报 (医 学 版)55卷8期 -冯致余,等.肺动脉吊带合并气管狭窄的外科治疗 \=-extubation. During the follow-up of 5 to 53 months, all patients in MS group were free of respiratory symptoms; 2 patients in SS group showed tachypnea during exercise; the respiratory symptoms were relieved during 5 to 11 months after surgery. Conclusion PA Sling complicated with tracheal stenosis has no specific symptoms and care should be taken to avoid misdiagnosis. Preoperative fiber optic bronchoscopy intervention contributes to evaluate the location, type and degree of lumen stenosis exactly. Moreover, selection of surgical approach depends on the severity and type of stenosis in tracheal lumen and preoperative respiratory symptoms.
    Feasibility and safety of transcatheter closure of ventricular septal defect with subaortic rim <2 mm
    ZHAO Lijian, HAN Bo, ZHANG Jianjun, YI Yingchun, JIANG Diandong, LÜ Jianli, WANG Jing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  52-56.  doi:10.6040/j.issn.1671-7554.0.2016.1145
    Abstract ( 819 )   PDF (927KB) ( 117 )   Save
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    Objective To explore the feasibility and safety of transcatheter closure of ventricular septal defect(VSD)with subaortic rim less than 2 mm. Methods The clinical data of 156 patients with VSD who underwent percutaneous interventional procedure during May 2010 and May 2016 were retrospectively reviewed. According to the subaortic rim by transthoracic echocardiography, the patients were divided into short rim group(<2 mm, n=69)and long rim group(≥2 mm, n=87). The success rate and complications were compared. The risk factors of failure in short rim group were studied with Logistic regression analysis. Results The success rate of short rim group and long rim group was 78.3% vs 96.6%(P<0.01). Complications in the short rim group included trivial aortic regurgitation in 5 cases, heart arrhythmia in 23 cases and trivial residual shunt without mechanical hemolysis in 6 cases. Complications in the long rim group included trivial aortic regurgitation in 1 case, heart arrhythmias in 14 cases, residual shunt in 6 cases and mechanical hemolysis in 2 cases. Logistic regression analysis revealed aortic valve prolapse before procedure(OR=8.8, 95%CI: 1.4-54.9, P=0.02)and defects ≥5 mm(OR=11.6, 95%CI: 2.37-57.11, P=0.003)were independent 山 东 大 学 学 报 (医 学 版)55卷8期 -赵立健,等.经导管介入治疗主动脉瓣下边缘<2 mm的室间隔缺损疗效评价 \=-risk factors of failure. Conclusion Transcatheter closure of VSD with subaortic rim <2 mm has relatively lower success rate. Short subaortic rim combined with aortic valve prolapse before procedure and defects ≥5 mm were independent risk factors of failure. Therefore, indications of interventional procedure for VSD with short subaortic rim should be strictly selected.
    Embedding strategy of pulmonary valvuloplasty for reconstruction of right ventricular outflow in tetralogy of Fallot
    TAN Qi, ZI Jie, LI Qingbao, LI Yong, LI Decai, SUI Runqian, SUN Liangong, YU Li, WANG Anbiao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  57-60.  doi:10.6040/j.issn.1671-7554.0.2016.1151
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    Objective To evaluate the effect of embedding strategy of pulmonary valvuloplasty for reconstruction of right ventricular outflow in tetralogy of Fallot. Methods The clinical data of 42 patients treated with this strategy during Sept. 2011 and June 2016 were retrospectively analyzed. During the surgery, the designed trapezoidal patch was inserted into dissected pulmonary valve in order to augment valve cusp and reconstruct valve ring. During the follow-up of 3~52 months, the pulmonary valve regurgitation(PVR)was evaluated. Results The surgery produced good short-term and medium-term effects, sustaining moderate PVR. Conclusion For patients with severe stenosis of pulmonary valve ring and dysplasia of pulmonary valve cusp, embedding strategy of pulmonary valve augmentation and annular extension improves PVR.
    Expression and clinical significance of angiopoietin-like protein 5 in human gastric cancer tissues
    NIE Siyue, WANG Zhen, SUN Jingyu, LIU Jie, LI Juan, WEI Xiaojuan, LU Chunxiao, L(¨overU)Xingyan, WANG Shuyun, SUN Yuping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  61-65.  doi:10.6040/j.issn.1671-7554.0.2017.105
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    Objective To investigate the expression of angiopoietin-like protein 5(ANGPTL5)and its clinical significance in primary human gastric cancer(GC). Methods Using immunohistochemistry(IHC), the expression of ANGPTL5 protein in 73 human specimens was detected including GC tissues and adjacent normal tissues. Pearson Chi-square test and continuity correction for Pearson Chi-square test were used to analyze the difference of ANGPTL5 expression between cancer tissues and adjacent normal tissues and the association between clinical pathological parameters and ANGPTL5 expression in GC tissues. Results ANGPTL5 was mainly expressed in cytoplasm of GC cells, and faint staining was presented in parts of interstitial cells, but most of adjacent normal gastric cells had no or little ANGPTL5 expression. A significant overexpression of ANGPTL5 protein was presented in GC tissues compared with that in adjacent normal tissues(χ2=38.939,P<0.001). Moreover, ANGPTL5 expression was significantly associated with primary tumor size(χ2=5.006, P=0.025), depth of tumor infiltration(χ2=6.810, P=0.009)and TNM stages(χ2=4.867, P=0.027). Furthermore, the overall survival(OS)in ANGPTL5 positive group was lower than that in the negative group(χ2=5.888,P=0.015). Conclusion ANGPTL5 was highly expressed in GC tissues, which was 山 东 大 学 学 报 (医 学 版)55卷8期 -聂思月,等.ANGPTL5在人胃癌组织中的表达及临床意义 \=-associated with poorly prognostic factors and shorter OS, suggesting that ANGPTL5 may be involved in GC progression, and it might be a new potential poor prognostic indicator for GC treatment.
    Imaging features of granular cell tumor in the alimentary system
    WANG Yuhong, ZHANG Lihong, WANG Linsheng, CHEN Yueqin, WANG Yanhui, WANG Jiehuan, LI Chuanfu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  66-70.  doi:10.6040/j.issn.1671-7554.0.2017.179
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    Objective To explore the imaging features of granular cell tumor in the alimentary system, in order to improve the understanding of this disease and reduce the rate of misdiagnosis. Methods The imaging findings of 10 cases of granular cell tumors in the alimentary system were retrospectively analyzed. Of the 10 cases, 5 underwent barium meal test, 9 underwent plain CT scan, 7 underwent enhanced CT scan, and 5 underwent plain and enhanced MRI. Results All of the 10 tumors were singular, including 4 in the esophagus, 3 in the stomach, 1 in duodenum, 1 in ileocecum and 1 in rectum. Barium meal images showed well-defined filling defect in 5 cases. Plain CT scan showed slightly low density in 9 cases, including 1 case with calcification, and 1 with focal necrosis. Of 7 cases underwent enhanced CT scan showed 6 masses were mild homogeneous intensification, and 1 moderate homogeneous intensification; all masses were gradually intensified on multiple-phase scan. Plain MRI showed long T1 and short T2 signals in 5 cases; enhanced MRI showed mild to moderate intensification. Conclusion The granular cell tumors in the alimentary system have certain imaging features: benign traits on barium meal, mild or moderate gradual intensification on CT and MRI, and T2WI signal on plain MRI.
    Application of computed tomography sagittal reconstruction for microwave ablation of hepatic carcinoma under the diaphragm
    WANG Ningning, QU Linli, CAO Fangli, LIANG Lei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  71-75.  doi:10.6040/j.issn.1671-7554.0.2016.1195
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    Objective To evaluate the efficacy of computed tomography(CT)sagittal reconstruction for microwave ablation of hepatic tumor under the diaphragm. Methods A total of 8 patients with primary liver cancer and 2 patients with hepatic metastases(altogether 11 lesions)who underwent microwave ablation with CT sagittal reconstruction were involved. During the follow-up of 36 months, the tumor clearance rate, local recurrence rate, survival rate, distant metastasis and changes of alpha fetal protein(AFP)were observed. Results The overall clearance rate was 90.9%. Of the 3 patients with preoperative AFP ≥ 200 ng/mL, 1 patient had AFP reduced to < 200 ng/mL. The 1-, 2- and 3-year local recurrence rate was 20%, 30% and 30%. The 1-, 2- and 3-year overall survival(OS)was 100%, 60% and 50%. No serious operation-related complications were observed. Conclusion Microwave ablation of hepatic carcinoma under the diaphragm with CT sagittal reconstruction is a preferred method if patients are unresectable.
    Early changes of pelvic floor function after transabdominal hysterectomy observed with transperineal pelvic ultrasound
    MENG Jian, MA Lin, KAN Yanmin, WANG Yuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  76-81.  doi:10.6040/j.issn.1671-7554.0.2016.1253
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    Objective To observe the early changes of pelvic floor function after transabdominal hysterectomy(TAH). Methods A total of 40 patients received transperineal pelvic ultrasound examination 1 week before and 3 months after TAH. The ultrasonic parameters of the resting state, contracting state and maximum Valsalva maneuver were recorded, including the rotation angle of the bladder neck(△vBA), posterior urethrovesical angle on the maximum Valsalva condition(vUJV), bladder neck, vaginal fornix and anorectal junction descent on the maximum Valsalva condition(vBND, vFND, vAND), decrease of levator hiatus on the maximum Valsalva maneuver and on the anal sphincter contraction(△vM, △cM), and so on. The parameters were compared to analyze the changes of pelvic floor function. Results According to the results of pelvic ultrasound, pelvic floor dysfunction after TAH was diagnosed in 7 cases, including stress urinary incontinence in 2 cases, proctoptoma in 3 cases, stress urinary incontinence complicated with bladder prolapse in 1 case, stress urinary incontinence complicated with bladder prolapse and proctoptoma in 1 case. The postoperative ultrasonic parameters such as △vBA, vUJV, △vUIA, vBND, vFND, vAND, vsB, vsF, vsA, and △vM were increased, indicating that the activity of pelvic organs increased, and the abdominal pressure resistant 山 东 大 学 学 报 (医 学 版)55卷8期 -孟健,等.经会阴盆底超声观察40例患者经腹子宫全切除术后早期盆底功能的变化 \=-function of pelvic floor reduced. In addition, the postoperative ultrasonic parameters including vxB, vDxB, vxF, vxA, △cxB, △cyB, and △cM decreased, showing that pelvic floor muscles and ligaments support ability abated, and the contraction of levator ani decreased. There were significant differences in all of the above parameters(P<0.05). Conclusion After TAH, the pelvic floor function weakened at the early stage. Transperineal pelvic ultrasound might monitor the pelvic floor function dynamically to detect the impairment before clinical symptoms appear, and to provide quantitative basis for early intervention, delay or prevention of pelvic floor functional disorder.
    Digital anatomic basis of parallel double screws in the posterior column of the acetabulum
    YU Ye, YANG Xianwei, XU Bo, YAN Qi, MU Weidong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  82-87.  doi:10.6040/j.issn.1671-7554.0.2016.1245
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    Objective To provide a safe zone for double screws in the posterior column and to obtain the relevant parameters of two screws. Methods CT scan data of 48 pelvic specimens were analyzed. With the help of Mimics 15.0, 2 parallel cylinders(to substitute for screws)were implanted to the posterior column of the right side acetabulum, and the relevant parameters were measured. Results Posterior cylinder: The average length(L1)was(130.88±6.11)mm in males and(121.47±5.23)mm in females; the average distance(D1)to the linea terminalis was(19.65±2.45)mm in males and(17.89±2.00)mm in females; the average distance(S1)to the junction between the anterior border of iliosacral articulation and linea terminalis of pelvis was(23.71±2.61)mm in males and(25.77±3.11)mm in females. Anterior cylinder: The average length(L2)was(125.25±5.49)mm in males and(116.37±4.83)mm in females; the average distance(D2)to the linea terminalis was(13.21±1.89)mm in males and(11.62±1.39)mm in females; the average distance(S2)to the junction between anterior border of iliosacral articulation and linea terminalis of pelvis was(24.36±3.95)mm in males and(28.15±4.70)mm in females. The average angle 山 东 大 学 学 报 (医 学 版)55卷8期 -余也,等.髋臼后柱平行双螺钉数字化解剖学分析 \=-between the cylinders and horizontal plane(α), sagittal plane(β), coronal plane(γ)was(66.18±4.40)°,(10.40±2.48)°,(20.89±5.05)° in males, and(69.01±5.25)°,(5.60±2.56)°,(19.91±5.58)° in females, respectively. The maximum radius of the cylinders was 6.00 mm. Conclusion There is sufficient space for 2 parallel cylinders with the same diameter in the posterior column of the acetabulum, with the maximum radius of the cylinders being less than 6.0 mm.
    Application of the geographical weighted regression model to explore the cause of stroke
    WANG Jichuan, LIU Ruihong, LI Dongzhi, XUE Fuzhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  88-94.  doi:10.6040/j.issn.1671-7554.0.2016.1437
    Abstract ( 807 )   PDF (5539KB) ( 168 )   Save
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    Objective To explore the spatial relationship between stroke and other diseases(myocardial infarction, malignant tumor, and infectious disease, et al)and investigate the common geographical risk factor among them. Methods The data of pathogenesis, all-cause mortality and demography of the patients with stroke and other diseases in Yiyuan County were collected from 2011 to 2014. Geographical weighted regression(GWR)model was constructed to analyze the spatial correlation between stroke and other disease. Results (1) The incidence density of myocardial infarction was positively associated with stroke, except in the northern region, and the coefficients were gradually weakened from west to east. (2) The incidence density of cancer was positively associated with stroke and the coefficients were gradually weakened from north to south. (3) The incidence density of infectious diseases was positively associated with stroke, except in the west region, and the coefficients were greater in central region than in west or east, and in central region, the coefficients were gradually weakened from north to south. Conclusion The spatial correlation between stroke and myocardial infarction, cancer and infectious diseases suggests that certain geographical and social factors may exist.
    Flexible and rigid ureteroscopy combined with holmium laser in the treatment of foreign body in the upper urinary tract
    JIAO Fangdong, CHEN Jun, LIU Ming, WANG Zhenyu, ZHANG Zhongxiao,WANG Dong, SHI Benkang, XU Zhishun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  95-98.  doi:10.6040/j.issn.1671-7554.0.2016.834
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    Objective To investigate the safety and efficacy of flexible and rigid ureteroscopy combined with holmium laser in the treatment of foreign body in the upper urinary tract. Methods The clinical data of 6 patients(4 male and 2 female)with iatrogenic foreign bodies in the upper urinary tract treated during Feb. 2014 and June 2016 were retrospectively analyzed. All of the foreign bodies were caused by indwelling of double J stent, one of which was residual stones caused by residue of double J stent. All cases underwent flexible and rigid ureteroscopy combined with holmium laser. Results All foreign bodies were removed successfully. No calculi in the kidney calices or abnormalities were observed. No complications occurred. Conclusion Flexible and rigid ureteroscopy combined with holmium laser is safe, effective and minimal invasive in the treatment of foreign body in the upper urinary tract.
    A preliminary study on the use of smart phone application Sina in the localization of supratentorial spaceoccupying lesions
    GUO Yu, MA Xiangyu, ZHANG Xuehai, SHI Chuanjun, LI Weiguo, CHEN Teng, XU Shujun, LI Xingang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  99-103.  doi:10.6040/j.issn.1671-7554.0.2016.1514
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    Objective To explore the use of smart phone application Sina in the localization of supratentorial spaceoccupying lesions, and to assess the deviation of Sina measurement compared with neuronavigation. Methods Before operation, Sina measurement and neuronavigation were used to locate lesions. The lesions averaged 2-6 cm in length, and the observation point was the center of the lesions. Results Compared with standard neuronavigation, Sina measurement had a deviation of(13.6±0.55)mm, which had statistical significance. The deviation had no difference in direction. Conclusion Although it has not yet become the neuronavigation equivalent, Sina can still be used in the localization of supratentorial spaceoccupying lesions and preoperative planning of the incision when neuronavigation is unavailable.
    Phytobezoar-induced small-bowel obstruction successfully treated with single-balloon enteroscopy: a case report
    MA Tian, LIU Guanqun, YU Yanbo, LI Yanqing, ZUO Xiuli
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2017, 55(8):  104-106.  doi:10.6040/j.issn.1671-7554.0.2017.366
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    Bezoars are not rare in northern China, especially in autumn and winter. Bezoar-induced small-bowel obstruction accounts for 4% cases of bowel obstruction. To our knowledge, only a few cases have been treated with balloon-assisted enteroscopy, and some have failed because of postoperative re-obstruction. In this paper, by introducing a case with phytobezoar-induced small-bowel obstruction successfully treated with single-balloon enteroscopy, we intend to show the procedures of endoscopic treatment and introduce our experience to prevent re-obstruction. Enteroscopy, an effective, safe, convenient and minimal invasive approach, can substitute surgery in the treatment of phytobezoar-induced small-bowel obstruction.