JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (8): 13-17.doi: 10.6040/j.issn.1671-7554.0.2017.490

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An investigation of endoplasmic reticulum stress and apoptosis in diabetic peripheral neuropathy

ZHANG Liang, XU Min, ZHUANG Xianghua, LOU Fuchen, LOU Nengjun, LÜ Li, GUO Wenjuan, ZHENG Fengjie, CHEN Shihong   

  1. Department of Endocrinology, The Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2017-05-31 Online:2017-08-10 Published:2017-08-10

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Abstract: Objective To observe endoplasmic reticulum stress(ERS)and apoptosis-related protein in diabetic peripheral neuropathy(DPN)in animal models and to investigate the possible mechanism. Methods A total of 36 Wistar rats were divided into 3 groups: control group(n=12, CON group), diabetic mellitus group (n=12, DM group), and diabetic peripheral neuropathy group(n=12, DPN group). The blood glucose(BG)and motor nerve conduction velocity(MNCV)were measured. The proteins, including CHOP, PERK, Bax, Bcl-2 and Caspase-12 were determined with Western blotting. Results Compared with CON group, the DM group had greatly increased BG and significantly decreased MNCV(both P<0.001); higher expression of CHOP, PERK, Bax, and Caspase-12(P=0.003, P<0.001, P<0.001, P<0.001), but lower expression of Bcl-2(P<0.001). Compared with the DM group, the DPN group had elevated expression of CHOP, PERK, Bax, and Caspase-12(P<0.001, P=0.001, P<0.001, P=0.002), but lower expression of Bcl-2(P<0.001). Conclusion In animal models of DPN, the ERS in sciatic nerve is increases, and the level of apoptosis also increases, indicating that ERS is involved in the occurrence of DPN.

Key words: Rats,Wistar, Motor nerve conduction velocity, Endoplasmic reticulum stress, Apoptosis, Diabetic peripheral neuropathy

CLC Number: 

  • R587.1
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