JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

Previous Articles     Next Articles

Role of decitabine in the mitochondria biogenesis of synchronized G0/G1 phase MDS-L cell line

QIU Zongjian, YANG Juan, SONG Qiang   

  1. Department Of Hematology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2013-11-08 Online:2014-05-10 Published:2014-05-10

PDF (PC)

170

Abstract: Objective  To investigate the effect of decitabin (DAC) on the mitochondrial biogenesis of synchronized G0/G1 cells. Methods  MDS-L cells were treated with aphidicolin (APC) to synchronize cell cycle at G0/G1 stage. After treatment of decitabine at different concentrations (0, 5, 10, 15μmol/L), reactive oxygen species (ROS) productions were detected by DCFH-DA. Changes of mitochondrial DNA copy number and expressions of coded genes,NADH dehydrogenase 1 (ND1) and NADH dehydrogenase 6(ND6), were detected with qRT-PCR.  Results  In comparison with the control group, decitabine at low concentration (5μmol/L) could promote the production of ROS (P<0.05) and increase the copy number of mtDNA (P<0.05); however, as the concentration increased to 15μmol/L, ROS production started to decline to even lower than that of the control (P<0.05). Besides, DAC could significantly change the expressions of ND1 and ND6 at high concentration. Conclusion  Decitabine can affect mitochondrial biogenesis by altering the mtDNA copy number and gene expressions in a concentration-dependent manner.

Key words: G0/G1 stage, Mitochondria, Reactive oxygen species, Decitabine

CLC Number: 

  • R551.3
[1] Chuanzhu YAN,Wei WANG,Kunqian JI,Yuying ZHAO. Mitochondrial dysfunction and related brain diseases [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 34-41.
[2] ZHAO Zuohui, LI Cuiling, WANG Daoguang, WANG Fengqin, QU Hongyi, DING Sentai, GONG Jing, LÜ Jiaju, YANG Jinghua. Effect of MnSOD acetylation on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 31-35.
[3] GOU Yun, ZHOU Bo, WEI Cao, CHEN Yunhua, XU Li, LIU Fen, ZHANG Chunlin, WEN Min. Protection of thioctic acid in mitochondria in the rat model of Parkinsons disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 18-23.
[4] LI Shuai, WANG Yalin, SUN Zhongwen, ZHU Meijia. Expression changes and mechanism of Nod-like receptor protein 3 inflammasome in the cerebral microvascular endothelial cells in type 2 diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 6-11.
[5] XIE Haibin, WU Qunzheng, LIU Shaozhuang, HUANG Xin, CHENG Yugang, HU Sanyuan, ZHANG Guangyong. Role of mitochondria-associated membranes in the improvement of insulin sensitivity in diabetic rats after sleeve gastrectomy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 36-40.
[6] WANG Xue, LI Qian, WANG Li, SUN Shuzhen, MA Aihua. CXCL16 gene silencing alleviates injury of mouse podocytes treated with the oxidized low-density lipoprotein [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 16-21.
[7] LUAN Haihui, XU Wei, WANG Muchuan, WU Lin, WANG Lingling, MA Jun, LIU Yiming. Clinical, pathological and molecular biological study on a Chinese family of Spinocerebellar Ataxia type 6 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(2): 63-67.
[8] GUO Changyan, QIN Xuejiao, WU Xiaoli, GAO Xue, CAI Keli. Protective effect of mitochondria-targeted peptide SS-31 against H2O2-induced apoptosis in human lens epithelial cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 23-26.
[9] LIU Hui, CHEN Tongshuai, LI Na, WANG Shujian, LI Jingyuan, BU Peili. Role of Sirt3 on the senescence of human umbilical vein endothelial cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 41-45.
[10] YANG Lu, ZHOU Yingze, NI Ming, FAN Xiushuang, MAN Jianmei, GUO Juntang. Beta-synuclein promotes the expression of vesicular monoamine transporter-2 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 61-64.
[11] ZHAO Xuelian, YU Jun, XIE Zhaohong, CAO Yanjun, LIU Zhen, WANG Xiao, XU Linlin, YANG Hui, ZHENG Xiaolei, SHEN Yang, BI Jianzhong. Mechanism of mitophagy in a cell model of Alzheimer's disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(10): 1-5.
[12] QIU Xiao-xue, CAO Li-li, YANG Xue, CHI Zhao-fu. Alteration of mitochondrial fission and fusion in hippocampus of  rats with status epilepticus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(7): 15-19.
[13] SUN Gao-ying1, WANG Xiao-yuan2, ZHANG Xiao-jin1, HAO Xiu-yu1， GUO Yuan-fang1, WANG Xiao1, BI Wen-xiang1. Effects of N-acetylcysteine  on resistance to paclitaxel in cervical carcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 42-47.
[14] JIANG Chong-hui1, AI Xiao-hong2, YANG Xiao-hong3, LUO Qiong3, CAO Jian-guo3. Generation of reactive oxygen species in apoptosis of human gastric cancer
SGC-7901 cells induced by 5,7-Dihydroxy-8-nitrochrysin [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 34-37.
[15] WANG Xiao-fei1, DING Ting-ting1, LIU Zhao1, JI Ping1,2, LIN Xue-fen1, QI Dong1, FENG Dan-dan1. Effect of hemimastication on the mitochondria of rat masseter muscle [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(12): 52-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd