JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (9): 31-35.doi: 10.6040/j.issn.1671-7554.0.2016.1604

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Effect of MnSOD acetylation on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O

ZHAO Zuohui1,2, LI Cuiling2, WANG Daoguang2, WANG Fengqin2, QU Hongyi1, DING Sentai3, GONG Jing2, LÜ Jiaju3, YANG Jinghua2   

  1. 1. Department of Pediatrics, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, Shandong, China;
    2. Cancer Research Center, School of Basic Medicine, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China;
    3. Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2016-12-02 Online:2017-09-10 Published:2017-09-10

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Abstract: Objective To investigate the deacetylation of manganese superoxide dismutase(MnSOD)regulated by silent information regulator 2-related enzymes(SIRT 3)in 786-O, a clear cell renal cell carcinoma(ccRCC)cell line, and its subsequent influences on cell proliferation and apoptosis viability. Methods Western blotting and immunoprecipitation 山 东 大 学 学 报 (医 学 版)55卷9期 -赵作辉,等.MnSOD乙酰化对肾透明细胞癌786-O细胞增殖、凋亡的影响 \=-(IP)were employed to confirm MnSOD acetylation in 786-O cells. Superoxide dismutase(SOD)activity kit, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay and Hoechst fluorescence staining solution were used to measure MnSOD enzymatic activity, cell proliferation and apoptosis viability, respectively. Results Compared with plasmid empty vector(pcDNA3.0), SIRT 3(pcDNA3.0-sirt3)deacetylated mitonchondrial MnSOD from 1.29±0.16 to 0.74±0.07 in 786-O cells(t=7.21, P<0.001), enhanced its enzymatic activity from(1.47±0.17)U/mg to(2.53±0.31)U/mg(t=6.70, P<0.001), increased cell proliferation rate from(25.28±5.75)% to(48.86±7.47)%(t=5.60, P<0.001), whereas cell apoptosis rate changed from(4.53±0.51)% to(4.45±0.59)%, which remained stable(t=0.24, P=0.82). Conclusion MnSOD deacetylation enhances the proliferation activity of ccRCC cell line 786-O, which indicates that MnSOD or its acetylation might be a potential therapeutic target for RCC.

Key words: Manganese superoxide dismutase, Deacetylase, Clear cell renal cell carcinoma, Reactive oxygen species, Targeted therapy

CLC Number: 

  • R737.11
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