JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 44-47.doi: 10.6040/j.issn.1671-7554.0.2014.490

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MRP8 regulates expression of IL-1β in gout cell model by NF-κB signaling pathway

ZHAO Lu1, SUN Junbo2, WEI Guimei1   

  1. 1. Department of Endocrinology, Third Affiliated Hospital of Henan College of Traditional Chinese Medicine, Zhengzhou 450008, Henan, China;
    2. Department of Medicine, Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450002, Henan, China
  • Received:2014-07-27 Revised:2015-01-23 Online:2015-06-10 Published:2015-06-10

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Abstract: Objective To explore the effect and underlying mechanism of myeloid-related protein 8 (MRP8) on gout. Methods Gout model in macrophages was induced by monosodium urate (MSU), and the expression of MRP8 in these model cells was detected by ELISA. Then, MRP8 siRNA was transferred into macrophage, and the effectiveness was verified by Western blotting. The influences of silenced MRP8 on the secretion of interleukin-1β (IL-1β), nuclear transcription factor-κB (NF-κB) and inhibitor of nuclear transcription factor-κB (I-κB) expressions in macrophages were detected by ELISA and Western blotting. Results The expression of MRP8 in macrophages increased after MSU induction (P<0.01) and MPR siRNA could silence MPR8 expression effectively. After the silence of MPR8, the IL-1β secretion decreased (P<0.05), NF-κB expression was down-regulated, while I-κB espression was up-regulated in macrophages. Conclusion MRP-8 participates in the process of IL-1β release in MSU-induced macrophages gout model, which may be regulated by NF-κB signal pathway.

Key words: Nuclear transcription factor-κB, Interleukin-1β, Inhibitor of nuclear transcription factor-κB, Myeloid-related protein 8, Macrophage

CLC Number: 

  • R589
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