JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 44-47.doi: 10.6040/j.issn.1671-7554.0.2014.490

Previous Articles     Next Articles

MRP8 regulates expression of IL-1β in gout cell model by NF-κB signaling pathway

ZHAO Lu1, SUN Junbo2, WEI Guimei1   

  1. 1. Department of Endocrinology, Third Affiliated Hospital of Henan College of Traditional Chinese Medicine, Zhengzhou 450008, Henan, China;
    2. Department of Medicine, Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450002, Henan, China
  • Received:2014-07-27 Revised:2015-01-23 Online:2015-06-10 Published:2015-06-10

PDF (PC)

270

Abstract: Objective To explore the effect and underlying mechanism of myeloid-related protein 8 (MRP8) on gout. Methods Gout model in macrophages was induced by monosodium urate (MSU), and the expression of MRP8 in these model cells was detected by ELISA. Then, MRP8 siRNA was transferred into macrophage, and the effectiveness was verified by Western blotting. The influences of silenced MRP8 on the secretion of interleukin-1β (IL-1β), nuclear transcription factor-κB (NF-κB) and inhibitor of nuclear transcription factor-κB (I-κB) expressions in macrophages were detected by ELISA and Western blotting. Results The expression of MRP8 in macrophages increased after MSU induction (P<0.01) and MPR siRNA could silence MPR8 expression effectively. After the silence of MPR8, the IL-1β secretion decreased (P<0.05), NF-κB expression was down-regulated, while I-κB espression was up-regulated in macrophages. Conclusion MRP-8 participates in the process of IL-1β release in MSU-induced macrophages gout model, which may be regulated by NF-κB signal pathway.

Key words: Nuclear transcription factor-κB, Interleukin-1β, Inhibitor of nuclear transcription factor-κB, Myeloid-related protein 8, Macrophage

CLC Number: 

  • R589
[1] Rammes A, Roth J, Goebeler M, et al. Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway[J]. J Biol Chem, 1997, 272(14): 9496-9502.
[2] Kerkhoff C, Klempt M, Sorg C, et al. Novel insights into structure and function of MRP8(S100A8) and MRP14 (S100A9)[J]. Biochim Biophys Acta, 1998, 1448(2): 200-211.
[3] Yong HY, Moon A. Roles of calcium-binding proteins, S100A8 and S100A9, in invasive phenotype of human gastric cancer cells[J]. Arch Pharm Res, 2007, 30(1):75-81.
[4] Colonna M. TREMs in the immune system and beyond[J]. Nat Rev Immunol, 2003, 3(6):445-453.
[5] Schiltz C, Liote F, Prudhommeaux F, et al. Monosodium urate monohydrate crystal-induced inflammation in vivo: quantitative histomorphometric analysis of cellular events[J]. Arthritis and rheumatism, 2002, 46(6):1643-1650.
[6] Burns CM, Wortmann RL. Gout therapeutics: new drugs for an old disease[J]. Lancet, 2011, 377(9760):165-177.
[7] 郑仲谨.核转录因子κB 在全身炎症反应综合征中的作用[J].免疫学杂志, 2001, 17(4):314-317. ZHENG Zhongjin. Function of nuclear factor of kappa B in the initiation and progression of systemic inflammatory response syndrome[J]. Immunological Journal, 2001, 17(4):314-317.
[8] 朱少芳,苏磊,何文,等.痛风的管理及用药策略[J].中国社区医师, 2011, 27: 13.
[9] 青玉凤,刘晶,周京国,等.原发性痛风患者326例血尿酸、胱抑素 C 和脂代谢特点分析及其临床意义[J].中华临床医师杂志:电子版, 2013, 7(7):2804-2808. QING Yufeng, LIU Jing, ZHOU Jingguo, et al. Altered serum uric acid, Cystatin C and lipids concentration and its linical significance in 326 primary gouty arthritis patients[J]. Chin J Clinicians: Electronic Edition, 2013, 7(7):2804-2808.
[10] 朱尚添.痛风研究进展[J].中国药物经济学, 2013, 9:508-510.
[11] Foell D, Wittkowski H, Hammerschmidt I, et al. Monitoring neutrophil activation in juvenile rheumatoid arthritis by S100A12 serum concentrations[J]. Arthritis Rheum, 2004, 50(4):1286-1295.
[12] Frosch M, Vogl T, Seeliger S, et al. Expression of myeloid—related proteins 8 and 14 in systemiconset juvenile rheumatoid arthritis[J]. Arthritis Rheum, 2003, 48(9): 2622-2626.
[13] Viemann D, Strey A, Janning A, et al. Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells[J]. Blood, 2005, 105(7):2955-2962.
[14] Loser K, Vogl T, Voskort M, et al. The Toll-like receptor 4 ligands Mrp8 and Mrp14 are crucial in the development of autoreactive CD8+ T cells[J]. Nat Med, 2010, 16(6):713-717.
[15] Van Lent P, Grevers L, Blom A, et al. Myeloid-related proteins S100A8/S100A9 regulate joint inflammation and cartilage destruction during antigen-induced arthritis[J]. Ann Rheum Dis, 2008, 67(12):1750-1758.
[16] Foell D, Wulffraat N, Wedderburn LR, et al. Methotrexate withdrawal at 6 vs 12 months in juvenile idiopathic arthritis in remission: a randomized clinical trial[J]. JAMA, 2010, 303(13):1266-1273.
[17] Holzinger D, Frosch M, Kastrup A, et al. The Toll-like receptor 4 agonist MRP8/14 protein complex is a sensitive indicator for disease activity and predicts relapses in systemic-onset juvenile idiopathic arthritis[J]. Ann Rheum Dis, 2012, 71(6):974-980.
[18] Wittkowski H, Kuemmerle-Deschner JB, Austermann J, et al. MRP8 and MRP14, phagocyte-specific danger signals, are sensitive biomarkers of disease activity in cryopyrin-associated periodic syndromes[J]. Ann Rheum Dis, 2011, 70(12):2075-2081.
[19] 向秋莲,张慈柳,彭镜, 等. TLR4、MRP8在内侧颞叶癫痫幼大鼠海马中表达的动态变化[J].中风与神经疾病杂志, 2012, 29(7):580-584. XIANG Qiulian, ZHANG Ciliu, PENG Jing, et al. Expression of TLR4 and MRP8 in the developing immature rats with mesial temporal lobe epilepsy[J]. J Apoplexy and Nervous Diseases, 2012, 29(7):580-584.
[20] Zimmer DB, Eubanks JO, Ramakrishnan D, et al. Evolution of the S100 family of calcium sensor proteins[J]. Cell calcium, 2013, 53(3):170-179.
[21] 谢蓓蓓,苏厚恒.MSU晶体介导的痛风性关节炎的炎症机制[J].中华临床医师杂志:电子版, 2013, 7(16):7518-7520.
[22] Khanna D, Fitzgerald JD, Khanna PP, et al. 2012 American College of Rheumatology guidelines for management of gout. Part 1: systematic nonpharmacologic and pharmacologic therapeutic approaches to hyperuricemia[J]. Arthritis Care Res (Hoboken), 2012, 64(10):1431-1446.
[23] Jiang Y, You XY, Fu KL, et al. Effects of extract from Mangifera indica leaf on monosodium urate crystal-induced gouty arthritis in rats[J]. Evid Based Complement Alternat Med, 2012, 2012:967573.
[24] Martinon F, Pétrilli V, Mayor A, et al. Gout-associated uric acid crystals activate the NALP3 inflammasome[J]. Nature, 2006, 440(7081): 237-241.
[25] Terkeltaub R, Sundy J, Schumacher H, et al. The interleukin 1 inhibitor rilonacept in treatment of chronic gouty arthritis: results of a placebo-controlled, monosequence crossover, non-randomised, single-blind pilot study[J]. Ann Rheum Dis, 2009, 68(10):1613-1617.
[25] Qing YF, Zhang QB, Zhou JG, et al. Changes in toll-like receptor (TLR)4-NFκB-IL1β signaling in male gout patients might be involved in the pathogenesis of primary gouty arthritis[J]. Rheumatol Int, 2014, 34(2):213-220.
[1] FU Jieqi, ZHANG Man, ZHANG Xiaolu, LI Hui, CHEN Hong. Molecular mechanism of Toll-like receptor 4 in the aggravation of blood lipid accumulation by inhibiting the peroxisome proliferator-activate receptor γ [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 24-31.
[2] CHEN Yikun, WANG Ketao, WANG Huayang, SHAO Qianqian, TAN Wanye, SONG Xiaobin, QU Xun, WEI Fengcai. Regulatory role of KIF4A in the expression of angiogenesis- associated factors of macrophages [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 39-43.
[3] YANG Yanjun, LIANG Jing, LI Fang, DU Lingxi. Effect of halofuginone on macrophage polarization in endometriosis mouse [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 26-31.
[4] ZONG Zhaoyun, LI Xia, HAN Zhenlong, WANG Xianteng, GUO Chun, ZHANG Lining, SHI Yongyu. Effect of tumor associated macrophages on the expression of c-Met in hepatocellular carcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 14-18.
[5] LIU Yang, WANG Huanliang, LIU Yayang, YAN Hongdan, SUN Baozhu, HUANG Shanshan, HUANG Rui, LEI Weifu. Effects of lidocaine on caspase-1 and IL-1β lipopolysaccharide-induced mice macrophages [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(12): 43-46,56.
[6] FENG Qing, TAN Xiaodong. L161982 influences macrophage subtypes in rats with experimental autoimmune neuritis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(10): 21-25.
[7] DONG Zhaojing, SHANG Qianwen, GUO Chun, ZHANG Lining, WANG Qun . Effect of PDCD4 siRNA on the lipid accumulation in macrophages#br# [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(4): 18-21.
[8] WANG Hao1, LI Xia2, WANG Chao3, LI Guosheng1, GUO Chun1, ZHU Faliang1, ZHANG Lining1, SHI Yongyu1. Role of tumor-associated macrophages in epithelial-mesenchymal transition of human hepatocellular carcinoma cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(4): 8-12.
[9] PAN Jiachao1,2, ZHANG Le1,2, XU Linlin3, SHAN Xiaoyu2, DU Wenjun2, CHEN Shijun2. Role of innate immunity induced by AIM2 in the pathogenesis of chronic hepatitis B [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(4): 74-79.
[10] LUO Jiangang, SUN Tao, LIN Xiaowen, LI Yun, ZHAO Fei, MIAO Guishen, FU Zhijian. Lentivirus mediated RNA interference inhibits NF-κB p65 gene expression in spinal rat cord neurons [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(3): 23-26.
[11] LIU Lu1, ZHANG Wen1, CHEN Han-xiang1, ZHENG Lin1, LU Yi2,WANG Hong1， TANG Wei1, ZHAO Wei-ming1. Inflammatory responses of PGRN-deficient peritoneal macrophage to bacterial lipopolysaccharide in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 58-62.
[12] LI Yan-ju, TAN Shu-hui, XIA Chun-feng, ZHANG Yong, DU Yi-feng, MA Guo-zhao. Effects of the nuclear transcription factor-κB on the expression of Kir6.2 and SUR1 subunits of KATP induced by Aβ1-42 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 5-9.
[13] WANG Xue-qin1, YUE Zhong-yong2, LI Ke-zhong1, MA Jin-feng1. Effects of cyclic mechanical stretch on the expression of TLR4 in alveolar macrophages of rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(06): 40-43.
[14] ZHANG Qing-lu, HOU Gui-hua, LIANG Ting, SONG Jing, ZHANG Chao. Effect and clinical significance of ISO-1 in the invasion
of ovarian cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(9): 33-.
[15] DONG Kun, ZHANG Xiang-li, SU Xue-lian, LIU Yuan. Expressions of peroxisome proliferator activated receptor alpha and
interleukin-1 beta mRNA in human pregnant myometrium [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(9): 105-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd