JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (10): 21-25.doi: 10.6040/j.issn.1671-7554.0.2015.068

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L161982 influences macrophage subtypes in rats with experimental autoimmune neuritis

FENG Qing, TAN Xiaodong   

  1. Department of Pediatric Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-01-16 Online:2015-10-10 Published:2015-10-10

Abstract: Objective To explore the effects of L161982, one prostaglandin E2-EP4 receptor antagonist, on macrophage subtypes in experimental autoimmune neuritis (EAN). Methods After 21 EAN models were established, they were randomly divided into group A, B and C. Rats in group A were intraperitoneally injected with 5 mg/kg L161982 dissolvent daily, rats in group B received the same volume from one day before immunization to the eighth day after immunization (immunization phase), and rats in group C received the same volume from the fifth to sixteenth day after immunization (onset phase). All rats were sacrificed on the sixteenth day after immunization. The infiltrated macrophages were concentrated in the lavage fluid of peritoneal exudates. The type M1 and M2 macrophages were identified using CD68 and CD86, or CD163 fluorescent antibodies, and measured with flow cytometry. The levels of IL-12 and 1L-10 in the single cell suspensions of mononuclear cells of spleen were measured with ELISA. Results Compared with group A, Group B and C had decreased percentage of CD68+ cells of total cells, increased percentage of CD163+ cells of CD68+ cells (P<0.05), decreased level of IL-12 (P<0.05), and increased level of IL-10 (P<0.05). Group B had decreased peak clinical score compared with group A and C (P<0.05). Conclusion L161982 attenuates EAN by contributing to the shift from M1 to M2 macrophages. The immunization phase has a more significant therapeutic effect.

Key words: Experimental autoimmune neuritis, Cytokine, Macrophages, L161982

CLC Number: 

  • R745.4
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